Dictyobacter alpinus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Ktedonobacteria; Ktedonobacterales; Dictyobacteraceae; Dictyobacter

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7631 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A402BBK7|A0A402BBK7_9CHLR HTH araC/xylS-type domain-containing protein OS=Dictyobacter alpinus OX=2014873 GN=KDA_42570 PE=4 SV=1
MM1 pKa = 7.15YY2 pKa = 9.21PQYY5 pKa = 11.09LPDD8 pKa = 3.62EE9 pKa = 4.73SVSFTPADD17 pKa = 3.64RR18 pKa = 11.84VPPSLAPDD26 pKa = 4.39ADD28 pKa = 3.87PVGDD32 pKa = 4.92VGPDD36 pKa = 2.71WLQYY40 pKa = 9.18YY41 pKa = 8.9QQDD44 pKa = 3.65NDD46 pKa = 3.52PSYY49 pKa = 9.32PASWLGQDD57 pKa = 3.36NPEE60 pKa = 3.57ASRR63 pKa = 11.84RR64 pKa = 11.84QRR66 pKa = 11.84HH67 pKa = 4.75QSLLAYY73 pKa = 9.9GWEE76 pKa = 4.01QEE78 pKa = 4.09EE79 pKa = 4.57WW80 pKa = 3.35

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A402B153|A0A402B153_9CHLR RNA polymerase subunit sigma-24 OS=Dictyobacter alpinus OX=2014873 GN=KDA_05520 PE=3 SV=1
MM1 pKa = 7.28VLTVLLVLRR10 pKa = 11.84VLSVSLALLALMVLLAHH27 pKa = 7.01KK28 pKa = 10.24ALLALTVRR36 pKa = 11.84LAHH39 pKa = 6.83KK40 pKa = 10.38ALLAQMVLTVPLVLKK55 pKa = 10.78VLLALLALTVLLVLLAHH72 pKa = 6.66KK73 pKa = 10.48VLLAQMVLMVLLAHH87 pKa = 6.71KK88 pKa = 10.42VLLAQMVPTVLLVLKK103 pKa = 10.3ALPALMALLARR114 pKa = 11.84KK115 pKa = 9.3VLLVLKK121 pKa = 8.72ATRR124 pKa = 11.84VTLVLLALLALLALPALTVLLALKK148 pKa = 9.91ALPALTVLLALKK160 pKa = 10.18ALPALMALLALKK172 pKa = 10.2ALPALMALLARR183 pKa = 11.84KK184 pKa = 9.26ALLVLKK190 pKa = 8.87ATRR193 pKa = 11.84VTLVLLALPVLMALLAHH210 pKa = 6.61KK211 pKa = 10.24VLSVLLALKK220 pKa = 10.51ALLVV224 pKa = 3.83

Molecular weight: 23.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7631

0

7631

2422746

39

6371

317.5

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.271 ± 0.029

0.952 ± 0.009

4.867 ± 0.021

5.734 ± 0.03

3.674 ± 0.021

6.951 ± 0.03

2.625 ± 0.014

6.089 ± 0.025

3.435 ± 0.022

10.976 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.308 ± 0.013

3.458 ± 0.021

5.049 ± 0.02

5.309 ± 0.031

5.789 ± 0.023

6.17 ± 0.024

6.022 ± 0.023

6.677 ± 0.025

1.495 ± 0.012

3.148 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski