Flavobacteria bacterium (strain BBFL7)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; unclassified Flavobacteriia

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2592 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q26I84|Q26I84_FLABB YfcH-like protein NAD dependent epimerase/dehydratase family OS=Flavobacteria bacterium (strain BBFL7) OX=156586 GN=BBFL7_01164 PE=3 SV=1
MM1 pKa = 7.77KK2 pKa = 10.45KK3 pKa = 9.86PLLLISFLFVGFLSIAQTANDD24 pKa = 4.03FVFDD28 pKa = 4.64SILANMWPFSISSSTGVTIDD48 pKa = 3.4WGDD51 pKa = 3.39GTTTNVPMGNNQTVPAHH68 pKa = 6.13SFSSTTRR75 pKa = 11.84TVIISGSIDD84 pKa = 3.51SIVFNLSSSSNNNPRR99 pKa = 11.84ITQWGSSQWKK109 pKa = 5.4TTEE112 pKa = 3.53WMFFDD117 pKa = 5.55CLYY120 pKa = 11.18LDD122 pKa = 4.54INASDD127 pKa = 3.94TPDD130 pKa = 3.91LSLCNSMAHH139 pKa = 6.02MFHH142 pKa = 7.29NAGQFNDD149 pKa = 5.7DD150 pKa = 3.34INNWDD155 pKa = 3.52VSNITDD161 pKa = 3.8MSDD164 pKa = 2.83LFYY167 pKa = 11.13GVYY170 pKa = 10.46SFNQALNNWDD180 pKa = 3.58VSSVTDD186 pKa = 3.29MSRR189 pKa = 11.84MFLYY193 pKa = 10.63ASNFNQPLSNWDD205 pKa = 3.42TANVINMGSMFQAASSFNSNINNWDD230 pKa = 3.24VSNVSNISHH239 pKa = 6.78MFYY242 pKa = 10.57NANSFNQDD250 pKa = 2.04ISNWDD255 pKa = 3.4VSNVNAIDD263 pKa = 3.62YY264 pKa = 9.92LFYY267 pKa = 10.77RR268 pKa = 11.84ATSFNQNISNWDD280 pKa = 3.23VSNITNMDD288 pKa = 3.22RR289 pKa = 11.84MFTIASSFNQNISSWDD305 pKa = 3.54VSNVTSMIEE314 pKa = 3.76MFEE317 pKa = 4.23YY318 pKa = 9.35ATSFNQPIDD327 pKa = 3.29NWDD330 pKa = 3.55VSNVTIMQDD339 pKa = 3.28MFVHH343 pKa = 5.96ATSFNQNISSWDD355 pKa = 3.5VSNVTDD361 pKa = 4.26MDD363 pKa = 4.17DD364 pKa = 3.16MFYY367 pKa = 11.03NATSFNQPLNNWDD380 pKa = 3.39FNQYY384 pKa = 10.26LNPRR388 pKa = 11.84RR389 pKa = 11.84FVAFSGLDD397 pKa = 3.24TNNYY401 pKa = 9.64DD402 pKa = 3.66KK403 pKa = 11.29LLQRR407 pKa = 11.84FVNLGMQYY415 pKa = 8.76KK416 pKa = 8.73TLNSFGLTYY425 pKa = 10.54CDD427 pKa = 3.07SFSRR431 pKa = 11.84NILLQNGWSINGDD444 pKa = 3.94SLDD447 pKa = 4.52LGCSALDD454 pKa = 3.58NQLQGSITYY463 pKa = 10.6DD464 pKa = 3.51FDD466 pKa = 6.0NNGCDD471 pKa = 3.69PNDD474 pKa = 4.53LIFSNVGIEE483 pKa = 3.99ISNGTTAFTLYY494 pKa = 10.54TDD496 pKa = 3.18INGDD500 pKa = 3.49YY501 pKa = 10.61SIPLSDD507 pKa = 3.33NTYY510 pKa = 8.94TVTPIIDD517 pKa = 3.66TNLFTTTPASATFTAANQNITTQDD541 pKa = 3.1FCLTATTPTDD551 pKa = 3.59DD552 pKa = 5.16LEE554 pKa = 4.18ITIIPLEE561 pKa = 4.05EE562 pKa = 4.1ARR564 pKa = 11.84PGFDD568 pKa = 2.83TNYY571 pKa = 10.82KK572 pKa = 10.08LVYY575 pKa = 9.26KK576 pKa = 10.87NKK578 pKa = 10.41GNTQLTGNIAFTYY591 pKa = 10.67DD592 pKa = 3.65DD593 pKa = 4.7DD594 pKa = 5.73FMDD597 pKa = 4.96FVSANPVVSSSSVGNLNWSFSNLDD621 pKa = 3.35PFEE624 pKa = 4.05TRR626 pKa = 11.84EE627 pKa = 3.91IDD629 pKa = 3.26FTLNMNTPTDD639 pKa = 3.78SNFPLNSNDD648 pKa = 3.54IISFTASITPSANDD662 pKa = 3.42ATPIDD667 pKa = 3.8NVFNLDD673 pKa = 3.48QTVVNSYY680 pKa = 11.02DD681 pKa = 3.92PNDD684 pKa = 3.69KK685 pKa = 9.89TCLQGEE691 pKa = 4.53TIEE694 pKa = 4.13PSMVGEE700 pKa = 4.32FVHH703 pKa = 6.22YY704 pKa = 10.22RR705 pKa = 11.84IRR707 pKa = 11.84FEE709 pKa = 4.37NEE711 pKa = 3.02GTASAINVRR720 pKa = 11.84IVDD723 pKa = 4.15YY724 pKa = 10.86IDD726 pKa = 3.34TSVFDD731 pKa = 4.39ISTLTPVSSSHH742 pKa = 7.63DD743 pKa = 3.73YY744 pKa = 10.51LAQITDD750 pKa = 3.6GNKK753 pKa = 10.37LEE755 pKa = 5.5FIFDD759 pKa = 4.27NINLPNTAPASQGYY773 pKa = 9.58ILFKK777 pKa = 10.6IKK779 pKa = 9.7TVDD782 pKa = 3.45TLVLGDD788 pKa = 3.98TFSNQAGIYY797 pKa = 9.9FDD799 pKa = 4.81FNFPIITNLEE809 pKa = 4.2TTTVAVTLSNNDD821 pKa = 2.76ITSLTMSLFPNPANDD836 pKa = 3.51TVTITSNVLFDD847 pKa = 3.31SCTIYY852 pKa = 10.94DD853 pKa = 4.12LRR855 pKa = 11.84GMLISSKK862 pKa = 10.57KK863 pKa = 9.77LQQPDD868 pKa = 3.44DD869 pKa = 3.68QYY871 pKa = 12.03KK872 pKa = 10.71FNIDD876 pKa = 3.34SLASGMYY883 pKa = 9.31MIQINNSQGSITQRR897 pKa = 11.84LIVEE901 pKa = 4.57

Molecular weight: 100.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q26D32|Q26D32_FLABB 30S ribosomal protein S3 OS=Flavobacteria bacterium (strain BBFL7) OX=156586 GN=rpsC PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.63LKK7 pKa = 10.48PVTPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGYY21 pKa = 9.53DD22 pKa = 4.32AITTDD27 pKa = 3.72KK28 pKa = 11.01PEE30 pKa = 4.39KK31 pKa = 10.55SLLAPKK37 pKa = 9.91KK38 pKa = 10.34RR39 pKa = 11.84SGGRR43 pKa = 11.84NASGRR48 pKa = 11.84MTMRR52 pKa = 11.84YY53 pKa = 9.61KK54 pKa = 10.94GGGHH58 pKa = 5.71KK59 pKa = 9.74RR60 pKa = 11.84RR61 pKa = 11.84YY62 pKa = 9.75RR63 pKa = 11.84IIDD66 pKa = 3.82FKK68 pKa = 10.86RR69 pKa = 11.84DD70 pKa = 3.53KK71 pKa = 10.96QGVPATIASIEE82 pKa = 3.99YY83 pKa = 10.19DD84 pKa = 3.48PNRR87 pKa = 11.84TAFIALVNYY96 pKa = 9.88QDD98 pKa = 3.39GEE100 pKa = 4.04KK101 pKa = 10.2RR102 pKa = 11.84YY103 pKa = 10.72VIAQNGLQVGQNIVSGDD120 pKa = 3.53SVAPEE125 pKa = 3.84VGNAMKK131 pKa = 10.58LSSIPLGSIISCIEE145 pKa = 3.55LHH147 pKa = 6.68PGQGAVIARR156 pKa = 11.84SAGSFAQLMARR167 pKa = 11.84DD168 pKa = 3.96GKK170 pKa = 10.33YY171 pKa = 9.08ATVKK175 pKa = 9.73MPSGEE180 pKa = 4.13TRR182 pKa = 11.84LVLQEE187 pKa = 4.05CLATIGAVSNSDD199 pKa = 3.15HH200 pKa = 6.2QLVVSGKK207 pKa = 9.63AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVVMNPVDD229 pKa = 3.47HH230 pKa = 7.05PMGGGEE236 pKa = 4.28GKK238 pKa = 10.51SSGGHH243 pKa = 4.59PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.3GYY255 pKa = 7.46RR256 pKa = 11.84TRR258 pKa = 11.84DD259 pKa = 3.23KK260 pKa = 11.38NKK262 pKa = 10.48ASTQYY267 pKa = 10.43ILEE270 pKa = 4.16RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.21KK274 pKa = 9.94

Molecular weight: 29.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2592

0

2592

928669

88

4500

358.3

40.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.754 ± 0.043

0.746 ± 0.021

6.328 ± 0.068

6.235 ± 0.047

4.843 ± 0.031

6.495 ± 0.049

1.879 ± 0.029

7.784 ± 0.048

6.561 ± 0.072

9.092 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.025

5.988 ± 0.05

3.429 ± 0.033

3.733 ± 0.028

3.623 ± 0.035

6.583 ± 0.048

6.123 ± 0.085

6.306 ± 0.036

1.041 ± 0.016

4.085 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski