Porcine associated porprismacovirus 10

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160HWI1|A0A160HWI1_9VIRU Replication associated protein OS=Porcine associated porprismacovirus 10 OX=2170117 PE=4 SV=1
MM1 pKa = 7.18AQSISVSVSEE11 pKa = 4.8TYY13 pKa = 10.77DD14 pKa = 3.3LSTTKK19 pKa = 10.69DD20 pKa = 3.34RR21 pKa = 11.84LGLIAIRR28 pKa = 11.84TPSMIAVNKK37 pKa = 9.67RR38 pKa = 11.84YY39 pKa = 9.49PGFIRR44 pKa = 11.84NFKK47 pKa = 9.18FLKK50 pKa = 10.24VKK52 pKa = 10.51SADD55 pKa = 3.56VVLSCASSLPADD67 pKa = 4.11PLQIGTTAGAIAPQDD82 pKa = 3.48MFNPLLYY89 pKa = 10.4KK90 pKa = 10.52AVSNDD95 pKa = 2.75SWNGLINRR103 pKa = 11.84IYY105 pKa = 10.89AGGLGLSSDD114 pKa = 3.96SLGGSVRR121 pKa = 11.84YY122 pKa = 8.58FQDD125 pKa = 3.75AFPQSSNSDD134 pKa = 3.6CMAMYY139 pKa = 10.62YY140 pKa = 10.62SLLSDD145 pKa = 3.77PSFKK149 pKa = 10.23KK150 pKa = 10.54AHH152 pKa = 5.18VQSGLEE158 pKa = 3.9MRR160 pKa = 11.84HH161 pKa = 6.51LIPLVYY167 pKa = 10.15HH168 pKa = 6.73LLSSGGVVPANNGAQIAGNASMNDD192 pKa = 3.17QVYY195 pKa = 10.41IGSNNIAGTSSILAYY210 pKa = 10.65NGAGSMKK217 pKa = 9.72GRR219 pKa = 11.84PVPMPPVEE227 pKa = 4.37CTPSVYY233 pKa = 10.46HH234 pKa = 5.53EE235 pKa = 4.26TQDD238 pKa = 3.38NVVRR242 pKa = 11.84GEE244 pKa = 3.85WTPTVGNIVPTYY256 pKa = 8.5VGCIIVPPASLNITYY271 pKa = 9.98FRR273 pKa = 11.84MTIRR277 pKa = 11.84WNLEE281 pKa = 3.73FFGVASDD288 pKa = 3.44IAKK291 pKa = 10.34ALPTSAVAISPYY303 pKa = 10.29TYY305 pKa = 10.48FSNQQTQQTLQASKK319 pKa = 10.47ISDD322 pKa = 3.59VSDD325 pKa = 3.27TAVNADD331 pKa = 3.68GDD333 pKa = 4.15DD334 pKa = 3.7GFARR338 pKa = 11.84SVEE341 pKa = 4.07ADD343 pKa = 3.49GVTLDD348 pKa = 5.71LIMEE352 pKa = 4.66HH353 pKa = 6.8

Molecular weight: 37.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160HWI1|A0A160HWI1_9VIRU Replication associated protein OS=Porcine associated porprismacovirus 10 OX=2170117 PE=4 SV=1
MM1 pKa = 6.16QTWVLTIPRR10 pKa = 11.84RR11 pKa = 11.84TEE13 pKa = 3.5IQVPTWLWLAWEE25 pKa = 4.34PVTIVSVCSKK35 pKa = 10.93KK36 pKa = 10.49LLNYY40 pKa = 9.89ILEE43 pKa = 4.21QSDD46 pKa = 3.42TKK48 pKa = 10.8RR49 pKa = 11.84YY50 pKa = 9.75IIGIEE55 pKa = 3.76KK56 pKa = 10.04GKK58 pKa = 10.54NGLEE62 pKa = 3.93HH63 pKa = 5.73FQIRR67 pKa = 11.84LSCSDD72 pKa = 3.68PEE74 pKa = 4.45FFEE77 pKa = 5.86HH78 pKa = 5.72MKK80 pKa = 9.98EE81 pKa = 3.88WYY83 pKa = 9.08PYY85 pKa = 10.28AHH87 pKa = 6.99IEE89 pKa = 4.22KK90 pKa = 9.97SDD92 pKa = 3.32VGINSEE98 pKa = 3.97SMEE101 pKa = 4.09YY102 pKa = 9.83EE103 pKa = 4.09RR104 pKa = 11.84KK105 pKa = 9.65EE106 pKa = 3.85GRR108 pKa = 11.84YY109 pKa = 4.94WTSMDD114 pKa = 3.08TTEE117 pKa = 5.26IRR119 pKa = 11.84IQRR122 pKa = 11.84FGKK125 pKa = 9.56PNRR128 pKa = 11.84TQQRR132 pKa = 11.84VLEE135 pKa = 4.16VLRR138 pKa = 11.84RR139 pKa = 11.84TNDD142 pKa = 3.12RR143 pKa = 11.84EE144 pKa = 3.99IVLWYY149 pKa = 9.88SDD151 pKa = 3.48KK152 pKa = 11.5GSIGKK157 pKa = 8.77SWLVGHH163 pKa = 6.85LWEE166 pKa = 4.45TGEE169 pKa = 5.42AYY171 pKa = 10.34VCQPQDD177 pKa = 3.08TVKK180 pKa = 10.81GMKK183 pKa = 9.55QDD185 pKa = 3.38VASEE189 pKa = 4.35YY190 pKa = 10.04IKK192 pKa = 10.85HH193 pKa = 5.78GWRR196 pKa = 11.84PCIVVDD202 pKa = 6.37LPRR205 pKa = 11.84TWKK208 pKa = 7.21WTKK211 pKa = 10.75DD212 pKa = 3.26LYY214 pKa = 11.06CALEE218 pKa = 4.27SIKK221 pKa = 11.02DD222 pKa = 3.74GLLKK226 pKa = 9.85DD227 pKa = 3.64TRR229 pKa = 11.84YY230 pKa = 10.8SSDD233 pKa = 3.33TINIKK238 pKa = 9.35GVKK241 pKa = 9.88VLVTSNTLPKK251 pKa = 9.98FDD253 pKa = 4.49SLSFDD258 pKa = 2.69RR259 pKa = 11.84WIVIEE264 pKa = 3.92NTIEE268 pKa = 3.83RR269 pKa = 11.84RR270 pKa = 11.84SFSS273 pKa = 3.28

Molecular weight: 32.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

626

273

353

313.0

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.911 ± 2.446

1.438 ± 0.236

5.591 ± 0.058

4.792 ± 1.959

3.035 ± 0.282

6.55 ± 0.852

1.597 ± 0.14

7.188 ± 0.522

5.112 ± 1.547

7.668 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.434

4.473 ± 0.925

4.952 ± 0.773

3.834 ± 0.102

4.952 ± 1.204

9.744 ± 1.231

6.39 ± 0.561

7.508 ± 0.329

2.556 ± 1.323

4.153 ± 0.074
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski