Microbacterium sp. NEAU-LLE

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3426 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371NRY0|A0A371NRY0_9MICO Uracil phosphoribosyltransferase OS=Microbacterium sp. NEAU-LLE OX=2293570 GN=upp PE=3 SV=1
MM1 pKa = 7.72PVTSTLLRR9 pKa = 11.84RR10 pKa = 11.84SLLALTAAAALTLAGCASNPGSDD33 pKa = 3.69PASAPSSSASEE44 pKa = 3.85PAADD48 pKa = 4.4GTCQYY53 pKa = 10.82PSDD56 pKa = 3.85GQAAKK61 pKa = 10.42QADD64 pKa = 3.89APAAEE69 pKa = 4.21PTVTGEE75 pKa = 3.78VSATIKK81 pKa = 10.12TSAGDD86 pKa = 3.56LAVTLDD92 pKa = 3.99ADD94 pKa = 3.93KK95 pKa = 10.93TPCTVNSFLSLAGQSYY111 pKa = 10.55FDD113 pKa = 3.36EE114 pKa = 4.86TQCHH118 pKa = 6.49RR119 pKa = 11.84LTTDD123 pKa = 4.33GIFVLQCGDD132 pKa = 3.43PTGTGTGGPGYY143 pKa = 10.58SFADD147 pKa = 3.72EE148 pKa = 4.29LSGDD152 pKa = 3.67EE153 pKa = 4.27TYY155 pKa = 10.98EE156 pKa = 4.2AGTLAMANAGPNTNGSQFFIVYY178 pKa = 9.95ADD180 pKa = 3.55SQLPPNYY187 pKa = 9.41TVFGHH192 pKa = 7.2LDD194 pKa = 3.48EE195 pKa = 5.63ASTAIVAGIAKK206 pKa = 10.2DD207 pKa = 3.65GTVTDD212 pKa = 4.98DD213 pKa = 4.32PNGSAPKK220 pKa = 10.12TPVTLLGVTQNN231 pKa = 3.58

Molecular weight: 23.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371NXH7|A0A371NXH7_9MICO ArsR family transcriptional regulator OS=Microbacterium sp. NEAU-LLE OX=2293570 GN=DY023_01730 PE=4 SV=1
MM1 pKa = 7.44PARR4 pKa = 11.84RR5 pKa = 11.84SAAVTRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84MRR16 pKa = 11.84RR17 pKa = 11.84LRR19 pKa = 11.84RR20 pKa = 11.84GHH22 pKa = 6.42RR23 pKa = 11.84SRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84PPRR30 pKa = 11.84LRR32 pKa = 11.84HH33 pKa = 5.49PCPSRR38 pKa = 11.84GPSPSRR44 pKa = 11.84RR45 pKa = 11.84PLGPPSCLPPGKK57 pKa = 10.02LSSRR61 pKa = 11.84RR62 pKa = 11.84LPGSPSRR69 pKa = 11.84RR70 pKa = 11.84PFTRR74 pKa = 11.84RR75 pKa = 11.84LRR77 pKa = 11.84RR78 pKa = 11.84PRR80 pKa = 11.84RR81 pKa = 11.84TSPSRR86 pKa = 3.25

Molecular weight: 10.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3426

0

3426

1128536

32

3002

329.4

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.619 ± 0.063

0.5 ± 0.01

6.435 ± 0.038

5.758 ± 0.035

3.13 ± 0.023

8.998 ± 0.038

1.988 ± 0.018

4.634 ± 0.028

2.038 ± 0.029

10.022 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.924 ± 0.019

1.906 ± 0.022

5.343 ± 0.032

2.827 ± 0.02

7.289 ± 0.042

5.398 ± 0.028

5.869 ± 0.042

8.768 ± 0.034

1.561 ± 0.016

1.991 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski