Haloarcula hispanica virus PH1

Taxonomy: Viruses; Varidnaviria; Helvetiavirae; Dividoviricota; Laserviricetes; Halopanivirales; Sphaerolipoviridae; Alphasphaerolipovirus

Average proteome isoelectric point is 5.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4JG84|M4JG84_9VIRU Uncharacterized protein OS=Haloarcula hispanica virus PH1 OX=1282967 GN=HhPH1_gp42 PE=4 SV=1
MM1 pKa = 7.54SPLSDD6 pKa = 3.66HH7 pKa = 7.55PIGTEE12 pKa = 3.5ARR14 pKa = 11.84LGQLTGDD21 pKa = 4.07DD22 pKa = 4.72ADD24 pKa = 3.94LLGYY28 pKa = 10.03FMADD32 pKa = 3.18TTSQGYY38 pKa = 10.01YY39 pKa = 10.53YY40 pKa = 9.98LTLRR44 pKa = 11.84TWEE47 pKa = 4.76HH48 pKa = 6.95PDD50 pKa = 5.56DD51 pKa = 5.05PDD53 pKa = 3.26AWTLSMRR60 pKa = 11.84YY61 pKa = 9.61AYY63 pKa = 8.74LTAGDD68 pKa = 5.15GGDD71 pKa = 3.27GWVDD75 pKa = 2.77EE76 pKa = 4.22GMNMRR81 pKa = 11.84VNGPRR86 pKa = 11.84EE87 pKa = 3.95PFLQALALMCAGAIPRR103 pKa = 11.84LDD105 pKa = 4.26QPDD108 pKa = 3.38ADD110 pKa = 3.35AWTGNVTEE118 pKa = 4.53EE119 pKa = 4.06

Molecular weight: 13.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4JGG1|M4JGG1_9VIRU Uncharacterized protein OS=Haloarcula hispanica virus PH1 OX=1282967 GN=HhPH1_gp15 PE=4 SV=1
MM1 pKa = 7.78DD2 pKa = 5.73RR3 pKa = 11.84SLDD6 pKa = 3.73GPKK9 pKa = 10.37PPGTRR14 pKa = 11.84TMNGAEE20 pKa = 3.95SLGIRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84PRR29 pKa = 11.84EE30 pKa = 3.82FLCARR35 pKa = 11.84DD36 pKa = 4.49LYY38 pKa = 10.52PAFHH42 pKa = 6.09QLIPRR47 pKa = 4.21

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

8835

23

1439

180.3

19.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.662 ± 0.668

0.872 ± 0.243

10.707 ± 0.541

8.489 ± 0.598

2.864 ± 0.242

9.938 ± 0.531

1.766 ± 0.266

2.671 ± 0.204

2.434 ± 0.248

6.429 ± 0.352

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.935 ± 0.224

2.92 ± 0.296

5.161 ± 0.354

3.543 ± 0.291

6.372 ± 0.441

5.048 ± 0.326

6.361 ± 0.378

7.199 ± 0.317

1.686 ± 0.183

2.943 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski