Sulfolobus islandicus filamentous virus 2

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Lipothrixviridae; Betalipothrixvirus; Sulfolobus islandicus filamentous virus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8BJA7|A0A1D8BJA7_SIFV SIFV.gp60-like protein OS=Sulfolobus islandicus filamentous virus 2 OX=1902331 GN=SIFV2_gp55 PE=4 SV=1
MM1 pKa = 7.77SFLLNLGQAFFSGLSSIATGVTSDD25 pKa = 3.51VTSVANGINYY35 pKa = 9.11AFNVLAKK42 pKa = 9.87FLQKK46 pKa = 10.56LPSEE50 pKa = 4.11IATFFQSIPGALVAFAHH67 pKa = 7.1AFGTYY72 pKa = 9.48LWDD75 pKa = 3.56GMQRR79 pKa = 11.84IASGFTTIMAPIEE92 pKa = 4.47RR93 pKa = 11.84GLEE96 pKa = 4.05TLGTTVINALTVVWNDD112 pKa = 2.43IKK114 pKa = 11.33AFASAIYY121 pKa = 10.3NSIVSMINNIVTFVQPFINDD141 pKa = 3.8LKK143 pKa = 10.65NAISFAYY150 pKa = 10.29NFLLNVINDD159 pKa = 3.49VYY161 pKa = 11.42SAFTIISNFFLDD173 pKa = 5.26LPTFFQNASNYY184 pKa = 10.02LNSLFTDD191 pKa = 4.43PGNQPNLLTMVPNLVASEE209 pKa = 4.07VSRR212 pKa = 11.84IAGAFPDD219 pKa = 4.22VIAYY223 pKa = 6.7NTFMEE228 pKa = 4.74VLPKK232 pKa = 9.45MVSGIASSPIFGNTVKK248 pKa = 10.84GMFAKK253 pKa = 10.37ALLMAGSPILSALLSEE269 pKa = 4.33LTKK272 pKa = 11.29ALMQSLFTSTQTTQTTQRR290 pKa = 11.84PSQPQIQPPSAPSTQLQQRR309 pKa = 11.84STQQTSLSDD318 pKa = 3.65LQNLQTQQLTPTQIEE333 pKa = 4.49VQLEE337 pKa = 4.24RR338 pKa = 11.84PNVTGVFTQDD348 pKa = 3.36VIGMGTASGGTAKK361 pKa = 10.53LVSGYY366 pKa = 10.59INFQNSIKK374 pKa = 10.4QFEE377 pKa = 4.59DD378 pKa = 3.38VFDD381 pKa = 3.53ITASFFMEE389 pKa = 5.74LIQSLQTEE397 pKa = 5.28FSQDD401 pKa = 2.89MAVNVSLTLLEE412 pKa = 4.75NIYY415 pKa = 10.33QVSQAIEE422 pKa = 4.28IIPSVDD428 pKa = 2.53ATTIVLPPGISLCNPSQAPSPSEE451 pKa = 4.02STSTSTNSIDD461 pKa = 3.18VTTTVDD467 pKa = 3.36VTEE470 pKa = 4.25QLCIPPYY477 pKa = 10.77DD478 pKa = 4.12LLADD482 pKa = 4.18GLTTQFAVIALLHH495 pKa = 6.18SNISDD500 pKa = 3.32VMLAITQLIYY510 pKa = 10.82GITFSNLISDD520 pKa = 4.35ILSASTQLVYY530 pKa = 10.89SITSNISTSDD540 pKa = 3.43TVSVSASLTPSPPPSEE556 pKa = 4.37SYY558 pKa = 10.01TYY560 pKa = 10.2YY561 pKa = 10.91VPIDD565 pKa = 3.49VAVTYY570 pKa = 7.72TIQIASSTTYY580 pKa = 10.69SGTLSYY586 pKa = 10.22TVSTSS591 pKa = 2.73

Molecular weight: 63.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8BJA2|A0A1D8BJA2_SIFV SIFV.gp55-like protein OS=Sulfolobus islandicus filamentous virus 2 OX=1902331 GN=SIFV2_gp50 PE=4 SV=1
MM1 pKa = 6.7VHH3 pKa = 7.08ALLSHH8 pKa = 6.73VITYY12 pKa = 7.67TKK14 pKa = 10.46FIVLVVKK21 pKa = 9.86LLIMSAIAGVIAGAFGGGIGGVGNAIGTIIGDD53 pKa = 3.99LEE55 pKa = 3.76RR56 pKa = 11.84AIARR60 pKa = 11.84FGGSIVNAFKK70 pKa = 10.39TVIDD74 pKa = 4.2KK75 pKa = 10.84VLSLAVRR82 pKa = 11.84IGRR85 pKa = 11.84IIEE88 pKa = 4.01KK89 pKa = 8.88YY90 pKa = 9.9FRR92 pKa = 11.84IAVHH96 pKa = 6.0YY97 pKa = 9.95IVLFLRR103 pKa = 11.84LAYY106 pKa = 9.74KK107 pKa = 10.54YY108 pKa = 9.04MYY110 pKa = 10.6SFYY113 pKa = 10.9TEE115 pKa = 3.97FQKK118 pKa = 11.18DD119 pKa = 3.44PWRR122 pKa = 11.84SLQFIGSMAILLNNSLFPP140 pKa = 4.81

Molecular weight: 15.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

11868

44

591

172.0

19.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.87 ± 0.304

1.07 ± 0.133

4.491 ± 0.267

6.741 ± 0.504

4.66 ± 0.199

4.971 ± 0.23

1.491 ± 0.148

9.319 ± 0.293

7.895 ± 0.646

9.378 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.134

6.041 ± 0.286

3.556 ± 0.214

3.446 ± 0.297

3.278 ± 0.279

6.48 ± 0.508

6.277 ± 0.531

7.095 ± 0.271

0.868 ± 0.114

5.747 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski