Ustilago hordei (strain Uh4875-4) (Barley covered smut fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago; Ustilago hordei

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2FWK5|I2FWK5_USTH4 Related to KOG1-Subunit of TORC1 a rapamycin-sensitive complex involved in growth control OS=Ustilago hordei (strain Uh4875-4) OX=1128400 GN=UHOR_01217 PE=3 SV=1
MM1 pKa = 7.55EE2 pKa = 5.15GFVNKK7 pKa = 10.17AKK9 pKa = 10.47EE10 pKa = 4.09FAGSDD15 pKa = 3.26QGKK18 pKa = 8.96NLLNQFTGGNKK29 pKa = 10.08NNDD32 pKa = 4.0DD33 pKa = 4.55NDD35 pKa = 3.73SSSYY39 pKa = 11.01GGNNNNSSGGFGSSRR54 pKa = 11.84RR55 pKa = 11.84NDD57 pKa = 3.44DD58 pKa = 3.53DD59 pKa = 4.89SYY61 pKa = 12.03NSSSNNDD68 pKa = 3.33SYY70 pKa = 12.01NSSSNKK76 pKa = 9.77DD77 pKa = 3.51SYY79 pKa = 11.78NSSSNNDD86 pKa = 3.59NDD88 pKa = 3.64SSSYY92 pKa = 10.85GSSGRR97 pKa = 11.84NNNNDD102 pKa = 3.47DD103 pKa = 3.91NYY105 pKa = 11.47GGGNNSSGYY114 pKa = 10.32GSSGRR119 pKa = 11.84NNDD122 pKa = 4.13DD123 pKa = 3.07GDD125 pKa = 4.35SYY127 pKa = 11.52GSSGRR132 pKa = 11.84AGNTSSGYY140 pKa = 11.47GNDD143 pKa = 3.42NDD145 pKa = 3.79NSYY148 pKa = 10.92GSSGGRR154 pKa = 11.84SGNSGSFGSSNKK166 pKa = 10.48DD167 pKa = 2.86EE168 pKa = 4.88DD169 pKa = 4.46SYY171 pKa = 11.89GSSGNRR177 pKa = 11.84GGNSGYY183 pKa = 11.27GNNDD187 pKa = 3.84DD188 pKa = 5.47NNDD191 pKa = 3.61SYY193 pKa = 11.88GSSARR198 pKa = 11.84GGSGGYY204 pKa = 9.76GGNSDD209 pKa = 4.98SYY211 pKa = 11.73SSSNNNNNDD220 pKa = 3.27YY221 pKa = 11.48

Molecular weight: 22.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2FVE8|I2FVE8_USTH4 Uncharacterized protein OS=Ustilago hordei (strain Uh4875-4) OX=1128400 GN=UHOR_06774 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 8.88KK8 pKa = 8.94RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LRR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 8.14RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.51MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7110

1

7111

4035239

25

6995

567.5

61.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.154 ± 0.028

1.054 ± 0.011

5.556 ± 0.019

5.815 ± 0.028

3.204 ± 0.016

6.863 ± 0.028

2.468 ± 0.014

4.174 ± 0.019

4.819 ± 0.028

8.854 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.976 ± 0.01

3.475 ± 0.015

6.097 ± 0.031

4.454 ± 0.024

6.16 ± 0.025

9.898 ± 0.044

5.934 ± 0.017

5.591 ± 0.019

1.197 ± 0.009

2.257 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski