Colletotrichum higginsianum (strain IMI 349063) (Crucifer anthracnose fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum; Colletotrichum destructivum species complex; Colletotrichum higginsianum

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H1W092|H1W092_COLHI Pyridoxine biosynthesis protein pyroA (Fragment) OS=Colletotrichum higginsianum (strain IMI 349063) OX=759273 GN=CH063_14833 PE=3 SV=1
RR1 pKa = 7.48PGSTEE6 pKa = 3.61AASPSSGTGLGSDD19 pKa = 5.12GEE21 pKa = 4.64CSCPAVTVTVPASTVYY37 pKa = 8.97VTIGGXAPAPTGAEE51 pKa = 3.91PSKK54 pKa = 9.67TASAQVPVTTGDD66 pKa = 2.97VDD68 pKa = 4.67ADD70 pKa = 3.98GDD72 pKa = 4.21DD73 pKa = 4.08SDD75 pKa = 6.24DD76 pKa = 4.59EE77 pKa = 4.41EE78 pKa = 7.09DD79 pKa = 3.89YY80 pKa = 11.46DD81 pKa = 4.53CPADD85 pKa = 3.88EE86 pKa = 4.57EE87 pKa = 5.11VVTLSTTVTVAPYY100 pKa = 6.97PTNGTASSGAAKK112 pKa = 8.46PTGYY116 pKa = 10.57YY117 pKa = 9.69KK118 pKa = 10.77RR119 pKa = 11.84SNKK122 pKa = 9.75LFF124 pKa = 3.86

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H1V7K0|H1V7K0_COLHI Psp1 domain-containing protein OS=Colletotrichum higginsianum (strain IMI 349063) OX=759273 GN=CH063_01453 PE=4 SV=1
PP1 pKa = 6.7STPSVTSVASTVPMATKK18 pKa = 10.28RR19 pKa = 11.84RR20 pKa = 11.84AASSTSTTATTTTPAKK36 pKa = 9.86RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.4SSKK42 pKa = 8.76TSPKK46 pKa = 9.99RR47 pKa = 11.84GSTSAPSPRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84KK59 pKa = 9.56SASPLIPALPAAQKK73 pKa = 9.83IPSMLPVRR81 pKa = 11.84ALL83 pKa = 3.26

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16110

0

16110

5876898

29

4139

364.8

40.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.177 ± 0.017

1.258 ± 0.009

5.763 ± 0.017

5.971 ± 0.022

3.765 ± 0.013

7.217 ± 0.019

2.353 ± 0.009

4.575 ± 0.013

4.679 ± 0.017

8.775 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.009

3.524 ± 0.012

6.075 ± 0.02

3.874 ± 0.016

6.225 ± 0.021

7.892 ± 0.02

5.907 ± 0.015

6.473 ± 0.018

1.523 ± 0.008

2.674 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski