Heterosigma akashiwo virus 01 (HaV01)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Raphidovirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q91DI1|Q91DI1_HAV01 UKCH-2 OS=Heterosigma akashiwo virus 01 OX=97195 GN=ukch-2 PE=4 SV=1
MM1 pKa = 7.28FVRR4 pKa = 11.84IVFLKK9 pKa = 10.69SLLILYY15 pKa = 10.15SNILMDD21 pKa = 4.5SLDD24 pKa = 3.63IRR26 pKa = 11.84DD27 pKa = 3.87PSNFTFTEE35 pKa = 4.45DD36 pKa = 3.28EE37 pKa = 4.09QKK39 pKa = 11.11EE40 pKa = 3.97IDD42 pKa = 3.76KK43 pKa = 10.66KK44 pKa = 10.9LNEE47 pKa = 5.28AIPEE51 pKa = 4.14LSDD54 pKa = 3.57KK55 pKa = 10.88NVNLEE60 pKa = 3.89TDD62 pKa = 3.68HH63 pKa = 7.9IKK65 pKa = 10.26IDD67 pKa = 3.78GQNFALISIVAPEE80 pKa = 4.13GLNQKK85 pKa = 8.76SNKK88 pKa = 9.29VCIKK92 pKa = 10.05IKK94 pKa = 10.69GVFDD98 pKa = 3.54TVEE101 pKa = 3.95NARR104 pKa = 11.84QHH106 pKa = 6.83ANDD109 pKa = 4.07LTKK112 pKa = 10.31HH113 pKa = 6.57DD114 pKa = 3.82PTFDD118 pKa = 3.5VFVVSLYY125 pKa = 10.48EE126 pKa = 3.95WLLVPPDD133 pKa = 3.45MDD135 pKa = 4.74KK136 pKa = 10.78ISEE139 pKa = 4.0QEE141 pKa = 4.2YY142 pKa = 10.9VDD144 pKa = 4.67EE145 pKa = 4.59EE146 pKa = 4.89LNTIITEE153 pKa = 4.48FKK155 pKa = 10.47KK156 pKa = 10.59SQKK159 pKa = 9.39QVQLEE164 pKa = 4.05FEE166 pKa = 4.3TRR168 pKa = 11.84KK169 pKa = 10.31DD170 pKa = 3.43ALRR173 pKa = 11.84SNIDD177 pKa = 2.99INTTIINDD185 pKa = 2.95EE186 pKa = 4.16DD187 pKa = 4.19MNVKK191 pKa = 9.14MLPNEE196 pKa = 4.05EE197 pKa = 5.12LGDD200 pKa = 4.16DD201 pKa = 3.6SDD203 pKa = 5.04GGVEE207 pKa = 3.89EE208 pKa = 4.56TKK210 pKa = 10.98GEE212 pKa = 4.07KK213 pKa = 9.78HH214 pKa = 6.02VSFSNDD220 pKa = 2.28IGTLLEE226 pKa = 4.59KK227 pKa = 10.66MNGTDD232 pKa = 4.57IGTLLEE238 pKa = 4.58KK239 pKa = 10.66MNGTDD244 pKa = 3.42SWCKK248 pKa = 10.96DD249 pKa = 3.43EE250 pKa = 7.32DD251 pKa = 5.87DD252 pKa = 6.3DD253 pKa = 7.56DD254 pKa = 6.74DD255 pKa = 7.23DD256 pKa = 6.55NDD258 pKa = 4.8DD259 pKa = 5.08DD260 pKa = 6.87DD261 pKa = 6.73DD262 pKa = 4.77IKK264 pKa = 11.15EE265 pKa = 4.46LKK267 pKa = 10.24NDD269 pKa = 3.75LKK271 pKa = 10.84IVV273 pKa = 3.47

Molecular weight: 31.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q91DI1|Q91DI1_HAV01 UKCH-2 OS=Heterosigma akashiwo virus 01 OX=97195 GN=ukch-2 PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 4.57LQIKK6 pKa = 10.22KK7 pKa = 10.03FDD9 pKa = 3.54NRR11 pKa = 11.84SVVDD15 pKa = 3.4KK16 pKa = 10.51RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.5FSSPIIVVIGKK30 pKa = 9.88RR31 pKa = 11.84NTGKK35 pKa = 10.22SEE37 pKa = 4.37LIKK40 pKa = 11.02NIMFCNSDD48 pKa = 3.41IPSGIIISPTEE59 pKa = 4.07SGNSFYY65 pKa = 11.29SDD67 pKa = 3.92FCPGIFVHH75 pKa = 6.02SQYY78 pKa = 11.36DD79 pKa = 3.39SEE81 pKa = 4.84LIKK84 pKa = 10.71KK85 pKa = 9.75IIKK88 pKa = 9.35RR89 pKa = 11.84QKK91 pKa = 10.79KK92 pKa = 9.6LIKK95 pKa = 10.25KK96 pKa = 9.35KK97 pKa = 10.59GKK99 pKa = 9.66HH100 pKa = 5.61PSNDD104 pKa = 2.84FFIILDD110 pKa = 3.63DD111 pKa = 4.15CMYY114 pKa = 10.79DD115 pKa = 2.92SKK117 pKa = 10.32TIGRR121 pKa = 11.84DD122 pKa = 2.9INIRR126 pKa = 11.84EE127 pKa = 3.74IFLNGRR133 pKa = 11.84HH134 pKa = 4.86YY135 pKa = 10.86QIFLILSLQYY145 pKa = 11.66VMDD148 pKa = 4.63LPVSLRR154 pKa = 11.84SNIDD158 pKa = 3.04YY159 pKa = 9.52VFCLRR164 pKa = 11.84EE165 pKa = 3.86NNLQNVEE172 pKa = 4.15KK173 pKa = 10.26LYY175 pKa = 11.15KK176 pKa = 10.21SFFGIFPSKK185 pKa = 10.5IFFNQAFSKK194 pKa = 9.22ITEE197 pKa = 4.21NYY199 pKa = 9.87GSIVLDD205 pKa = 3.16NTSRR209 pKa = 11.84SNRR212 pKa = 11.84IDD214 pKa = 3.24EE215 pKa = 4.63CIFWFRR221 pKa = 11.84ATFPLPNFRR230 pKa = 11.84IGNKK234 pKa = 9.74KK235 pKa = 8.38LWKK238 pKa = 9.14MHH240 pKa = 6.23DD241 pKa = 3.32KK242 pKa = 10.92YY243 pKa = 11.57YY244 pKa = 10.9EE245 pKa = 4.37DD246 pKa = 4.11VSDD249 pKa = 3.88ASEE252 pKa = 4.4DD253 pKa = 3.75EE254 pKa = 4.56KK255 pKa = 11.23EE256 pKa = 4.36SKK258 pKa = 10.97AFDD261 pKa = 4.75DD262 pKa = 4.33IKK264 pKa = 10.42TKK266 pKa = 10.3TSQKK270 pKa = 10.71LKK272 pKa = 10.08TILTNKK278 pKa = 8.38TKK280 pKa = 10.88

Molecular weight: 32.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2137

273

1144

534.3

61.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.463 ± 1.057

1.451 ± 0.253

6.27 ± 1.702

7.534 ± 1.061

5.335 ± 0.509

4.82 ± 0.68

1.919 ± 0.288

8.985 ± 0.825

8.142 ± 1.464

8.049 ± 0.659

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.854 ± 0.624

6.785 ± 0.277

3.369 ± 0.184

3.369 ± 0.523

4.633 ± 0.635

6.036 ± 0.744

6.177 ± 0.72

5.615 ± 0.354

1.029 ± 0.113

4.165 ± 0.706
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski