Kitasatospora sp. MMS16-BH015

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Kitasatospora; unclassified Kitasatospora

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5AVW3|A0A2H5AVW3_9ACTN Uncharacterized protein OS=Kitasatospora sp. MMS16-BH015 OX=2018025 GN=CFP65_1373 PE=4 SV=1
MM1 pKa = 7.66SSEE4 pKa = 4.05ALEE7 pKa = 4.02VWIDD11 pKa = 3.41QDD13 pKa = 3.99LCTGDD18 pKa = 4.65GICVQYY24 pKa = 10.96APEE27 pKa = 4.18VFEE30 pKa = 5.9LDD32 pKa = 3.05IDD34 pKa = 3.65GLAYY38 pKa = 10.69VKK40 pKa = 11.17GEE42 pKa = 4.15DD43 pKa = 4.26DD44 pKa = 4.55EE45 pKa = 5.93LRR47 pKa = 11.84QKK49 pKa = 10.68PGEE52 pKa = 4.25TVLVPLTILQDD63 pKa = 3.64VVDD66 pKa = 4.55SVKK69 pKa = 10.7DD70 pKa = 3.64CPGEE74 pKa = 4.35CIHH77 pKa = 6.3VRR79 pKa = 11.84RR80 pKa = 11.84AADD83 pKa = 3.45GVEE86 pKa = 4.5VYY88 pKa = 10.92GPDD91 pKa = 3.44ADD93 pKa = 3.69

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5B1L6|A0A2H5B1L6_9ACTN Cyclase I OS=Kitasatospora sp. MMS16-BH015 OX=2018025 GN=CFP65_3509 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84SDD5 pKa = 3.94PLRR8 pKa = 11.84HH9 pKa = 5.82HH10 pKa = 7.06LRR12 pKa = 11.84RR13 pKa = 11.84VRR15 pKa = 11.84SQHH18 pKa = 5.42RR19 pKa = 11.84ARR21 pKa = 11.84RR22 pKa = 11.84PHH24 pKa = 5.86TGHH27 pKa = 7.13RR28 pKa = 11.84SHH30 pKa = 7.17VGSTHH35 pKa = 5.9RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84AGAHH42 pKa = 4.69GHH44 pKa = 4.75HH45 pKa = 6.94HH46 pKa = 6.58KK47 pKa = 10.31AAHH50 pKa = 5.12HH51 pKa = 6.3HH52 pKa = 6.19RR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 5.89HH56 pKa = 5.61PRR58 pKa = 11.84GPRR61 pKa = 11.84AHH63 pKa = 6.76HH64 pKa = 6.47LKK66 pKa = 9.84HH67 pKa = 5.75HH68 pKa = 6.85AKK70 pKa = 10.2GKK72 pKa = 9.08HH73 pKa = 5.3LRR75 pKa = 11.84HH76 pKa = 5.67AHH78 pKa = 5.58RR79 pKa = 11.84VRR81 pKa = 11.84HH82 pKa = 5.79AGRR85 pKa = 11.84HH86 pKa = 3.75RR87 pKa = 11.84HH88 pKa = 4.78RR89 pKa = 11.84THH91 pKa = 4.89VQHH94 pKa = 6.99RR95 pKa = 11.84RR96 pKa = 11.84HH97 pKa = 5.07QRR99 pKa = 11.84RR100 pKa = 11.84EE101 pKa = 3.83HH102 pKa = 5.44RR103 pKa = 11.84HH104 pKa = 4.94AAHH107 pKa = 7.14LHH109 pKa = 5.38RR110 pKa = 11.84KK111 pKa = 8.99RR112 pKa = 11.84RR113 pKa = 11.84TAAHH117 pKa = 6.63RR118 pKa = 11.84RR119 pKa = 11.84TLHH122 pKa = 4.99HH123 pKa = 6.84HH124 pKa = 6.88RR125 pKa = 11.84LQAAHH130 pKa = 5.2QRR132 pKa = 11.84HH133 pKa = 5.04RR134 pKa = 11.84RR135 pKa = 11.84RR136 pKa = 11.84RR137 pKa = 11.84HH138 pKa = 4.1MARR141 pKa = 11.84HH142 pKa = 4.67QAAHH146 pKa = 6.23HH147 pKa = 6.12RR148 pKa = 11.84RR149 pKa = 11.84HH150 pKa = 5.85AAAHH154 pKa = 5.91HH155 pKa = 6.45RR156 pKa = 11.84GAHH159 pKa = 4.85HH160 pKa = 4.65QHH162 pKa = 6.43RR163 pKa = 11.84RR164 pKa = 11.84GQAHH168 pKa = 6.73RR169 pKa = 11.84SNRR172 pKa = 11.84RR173 pKa = 11.84YY174 pKa = 10.05RR175 pKa = 3.45

Molecular weight: 21.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7193

0

7193

2544074

45

6745

353.7

37.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.391 ± 0.05

0.798 ± 0.009

5.324 ± 0.021

5.501 ± 0.036

2.625 ± 0.017

9.659 ± 0.033

2.306 ± 0.016

2.868 ± 0.018

1.883 ± 0.021

11.006 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.479 ± 0.011

1.786 ± 0.022

6.446 ± 0.029

3.061 ± 0.018

7.548 ± 0.038

5.018 ± 0.03

6.469 ± 0.038

8.178 ± 0.027

1.513 ± 0.011

2.14 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski