Pithovirus sibericum

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified dsDNA viruses; Pithoviridae; Pithovirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 467 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5S5E6|W5S5E6_9VIRU Rab-like GTPase OS=Pithovirus sibericum OX=1450746 GN=pv_380 PE=4 SV=1
MM1 pKa = 7.42NLSQFEE7 pKa = 4.77GPTGPTGPTGPNGPIGSTGAIGATGATGISGPTGPTGPIGSTGAIGATGATGISGPTGPIGFTGSTGPVGNTGEE81 pKa = 4.28VGSVGPNGNTGSTGPSGEE99 pKa = 4.27IGSTGPTGAIGSTGATGDD117 pKa = 3.77TGPTGPIGDD126 pKa = 3.93TGAIGSTGATGISGPTGPIGEE147 pKa = 4.57VGPTGPIGATGATGIVGATGPVGATGDD174 pKa = 3.74PGGTGPVGSGFFASSFSGIRR194 pKa = 11.84DD195 pKa = 3.3GGDD198 pKa = 3.02FVVNGNTPTVITGYY212 pKa = 10.56GIYY215 pKa = 10.02IPNPDD220 pKa = 4.43FDD222 pKa = 4.74PSTGIFAPFSVEE234 pKa = 3.75GATGNYY240 pKa = 8.85HH241 pKa = 6.81LLVNVRR247 pKa = 11.84SEE249 pKa = 4.01SNYY252 pKa = 10.63AFGQGIRR259 pKa = 11.84IDD261 pKa = 4.56IFDD264 pKa = 3.61QTDD267 pKa = 3.62SIVIAFQEE275 pKa = 5.26FIFQQASIISPLQIALTLNYY295 pKa = 9.68IGPLEE300 pKa = 4.58DD301 pKa = 2.99LHH303 pKa = 8.94SYY305 pKa = 8.06VARR308 pKa = 11.84FTNINVASSTILNSTFSYY326 pKa = 10.93GGFLIGG332 pKa = 5.05

Molecular weight: 31.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5S6H8|W5S6H8_9VIRU Uncharacterized protein OS=Pithovirus sibericum OX=1450746 GN=pv_376 PE=4 SV=1
MM1 pKa = 7.42SNVPTILSVVLSGAAGVFASMAWKK25 pKa = 10.33DD26 pKa = 3.36ADD28 pKa = 3.6GEE30 pKa = 4.23ARR32 pKa = 11.84KK33 pKa = 8.9WLGWGTFICFIPVLLLVIAIVLLLFTFGLSLFLLTLLGVASITLIIGAGFLIYY86 pKa = 9.13GTILLASIEE95 pKa = 4.36DD96 pKa = 3.68RR97 pKa = 11.84TSKK100 pKa = 10.69QNSALRR106 pKa = 11.84NAIIASSIAGVGALFIILSLLLRR129 pKa = 11.84LSRR132 pKa = 11.84SRR134 pKa = 11.84QSSRR138 pKa = 11.84KK139 pKa = 9.14

Molecular weight: 14.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

467

0

467

137411

49

1643

294.2

33.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.631 ± 0.103

2.104 ± 0.09

4.771 ± 0.073

7.432 ± 0.134

6.144 ± 0.107

5.899 ± 0.18

1.674 ± 0.05

6.636 ± 0.073

5.879 ± 0.131

10.092 ± 0.131

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.62 ± 0.037

4.676 ± 0.077

4.848 ± 0.115

4.237 ± 0.073

5.246 ± 0.078

9.094 ± 0.133

4.745 ± 0.137

5.577 ± 0.073

1.295 ± 0.055

3.399 ± 0.073
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski