EBPR podovirus 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8TUN5|F8TUN5_9CAUD Uncharacterized protein gp33 OS=EBPR podovirus 1 OX=1048515 GN=gp33 PE=4 SV=1
MM1 pKa = 6.47TTALQLVTSALRR13 pKa = 11.84KK14 pKa = 9.32IGAVAAGEE22 pKa = 4.3APDD25 pKa = 5.19ASEE28 pKa = 5.01QSDD31 pKa = 3.5ALAALNQLIEE41 pKa = 4.12SWNLQGLTLYY51 pKa = 10.65RR52 pKa = 11.84LEE54 pKa = 4.35NASYY58 pKa = 9.68TLVPSQQTYY67 pKa = 9.42TIGSGADD74 pKa = 3.23FDD76 pKa = 4.23GARR79 pKa = 11.84PITLNGAFVTRR90 pKa = 11.84GGIDD94 pKa = 3.64YY95 pKa = 9.75PVVPLTQQQWNDD107 pKa = 2.84ILQKK111 pKa = 9.7STEE114 pKa = 4.25SQLPEE119 pKa = 3.53AVYY122 pKa = 10.5YY123 pKa = 10.6EE124 pKa = 4.19PTFPDD129 pKa = 3.2GTLRR133 pKa = 11.84FWPVPLEE140 pKa = 4.09ALTVTLAINMQLGAVADD157 pKa = 4.11INDD160 pKa = 4.6DD161 pKa = 3.8LAFPPGYY168 pKa = 10.22EE169 pKa = 3.78RR170 pKa = 11.84ALVYY174 pKa = 10.88ALAVEE179 pKa = 4.71LAPEE183 pKa = 4.26YY184 pKa = 10.24PAVTLSQRR192 pKa = 11.84VIDD195 pKa = 4.51AADD198 pKa = 3.37EE199 pKa = 4.09ALADD203 pKa = 3.65IKK205 pKa = 10.84RR206 pKa = 11.84ANNTQNQAATFDD218 pKa = 3.3IALAGGCGGSLAAFVAGYY236 pKa = 10.29

Molecular weight: 25.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8TUK3|F8TUK3_9CAUD Uncharacterized protein gp1 OS=EBPR podovirus 1 OX=1048515 GN=gp1 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 9.73WLRR5 pKa = 11.84VNNFIRR11 pKa = 11.84AGEE14 pKa = 4.1LNTNRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84LTSNCAIRR29 pKa = 11.84TLPVMRR35 pKa = 11.84KK36 pKa = 7.95HH37 pKa = 5.8QVIEE41 pKa = 4.09AFGTQAEE48 pKa = 4.32AARR51 pKa = 11.84ALGCYY56 pKa = 9.45PSTIHH61 pKa = 6.49EE62 pKa = 4.25WDD64 pKa = 3.51EE65 pKa = 4.42VPDD68 pKa = 3.7GRR70 pKa = 11.84QYY72 pKa = 10.94QIEE75 pKa = 4.04IATRR79 pKa = 11.84GRR81 pKa = 11.84LKK83 pKa = 10.86ADD85 pKa = 3.85LPADD89 pKa = 3.61RR90 pKa = 11.84RR91 pKa = 11.84ALLKK95 pKa = 10.34VKK97 pKa = 10.62KK98 pKa = 9.84SGRR101 pKa = 11.84SVKK104 pKa = 9.97SVKK107 pKa = 10.22SRR109 pKa = 11.84AGLRR113 pKa = 3.48

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

10622

48

703

193.1

21.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.651 ± 0.745

1.29 ± 0.175

5.658 ± 0.252

6.788 ± 0.546

2.975 ± 0.174

8.172 ± 0.443

1.544 ± 0.14

4.566 ± 0.19

4.566 ± 0.29

7.55 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.448 ± 0.204

3.229 ± 0.267

4.331 ± 0.32

4.397 ± 0.327

7.061 ± 0.366

6.308 ± 0.306

5.526 ± 0.464

5.988 ± 0.288

1.572 ± 0.171

2.382 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski