Microviridae sp. ctYqV29

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 8.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W5J3|A0A5Q2W5J3_9VIRU Putative minor capsid protein OS=Microviridae sp. ctYqV29 OX=2656700 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.06RR3 pKa = 11.84SKK5 pKa = 10.74FSLSNYY11 pKa = 9.7KK12 pKa = 10.33LLSCDD17 pKa = 2.93MGEE20 pKa = 4.67LVPCGLTEE28 pKa = 4.12VLPGDD33 pKa = 4.89TIQQATSCLLRR44 pKa = 11.84ASPLLSPVMHH54 pKa = 6.64PVDD57 pKa = 3.48VRR59 pKa = 11.84LHH61 pKa = 5.34HH62 pKa = 6.8WYY64 pKa = 10.27VPHH67 pKa = 6.81RR68 pKa = 11.84LVWEE72 pKa = 4.39DD73 pKa = 3.96FEE75 pKa = 6.02DD76 pKa = 5.54FITGGSDD83 pKa = 3.21GLDD86 pKa = 2.63ASVFPTITIGGGSGAAVGSLADD108 pKa = 3.82YY109 pKa = 10.83LGVPTGVNNIEE120 pKa = 4.13VSALPFRR127 pKa = 11.84GYY129 pKa = 11.66AMIWNEE135 pKa = 3.74WYY137 pKa = 10.17RR138 pKa = 11.84DD139 pKa = 3.38QDD141 pKa = 3.76LQTALAISEE150 pKa = 4.23GSGADD155 pKa = 3.43TTTSTVLQNCSWEE168 pKa = 3.99KK169 pKa = 11.18DD170 pKa = 3.58YY171 pKa = 9.31FTSSRR176 pKa = 11.84PWEE179 pKa = 4.19QKK181 pKa = 10.24GPAVTVPLGDD191 pKa = 3.58TAPVDD196 pKa = 4.56GISMRR201 pKa = 11.84NLGNAANNAGQSYY214 pKa = 8.86GAQAASDD221 pKa = 4.26YY222 pKa = 11.5GPGTTPTWRR231 pKa = 11.84ADD233 pKa = 3.53TQEE236 pKa = 4.17LYY238 pKa = 10.38IRR240 pKa = 11.84AQSNAVSGTGNRR252 pKa = 11.84PQIFADD258 pKa = 4.54LSNASAITVGALRR271 pKa = 11.84EE272 pKa = 4.07AMALQRR278 pKa = 11.84YY279 pKa = 8.41EE280 pKa = 3.73EE281 pKa = 3.96ARR283 pKa = 11.84ARR285 pKa = 11.84FGSRR289 pKa = 11.84YY290 pKa = 9.35VEE292 pKa = 3.84YY293 pKa = 10.7LRR295 pKa = 11.84YY296 pKa = 10.15LGVQSSDD303 pKa = 3.08ARR305 pKa = 11.84LQRR308 pKa = 11.84PEE310 pKa = 3.7YY311 pKa = 10.32LGGGRR316 pKa = 11.84EE317 pKa = 4.1TIQWSEE323 pKa = 3.99VLQTAEE329 pKa = 3.95GTDD332 pKa = 3.69PVGSLKK338 pKa = 10.9GHH340 pKa = 7.35GISSMRR346 pKa = 11.84SNRR349 pKa = 11.84YY350 pKa = 8.17RR351 pKa = 11.84RR352 pKa = 11.84YY353 pKa = 9.24IEE355 pKa = 3.61EE356 pKa = 3.87HH357 pKa = 6.26GYY359 pKa = 10.9VYY361 pKa = 10.58TFLSVRR367 pKa = 11.84PKK369 pKa = 10.02TIYY372 pKa = 10.16AQGLARR378 pKa = 11.84HH379 pKa = 5.55WNRR382 pKa = 11.84RR383 pKa = 11.84VKK385 pKa = 10.65EE386 pKa = 4.15DD387 pKa = 3.37FWQKK391 pKa = 9.59EE392 pKa = 4.02LQFIGQQEE400 pKa = 4.33VLNKK404 pKa = 9.76EE405 pKa = 4.16VYY407 pKa = 9.55AAHH410 pKa = 7.33ASPNNVFGYY419 pKa = 8.45QDD421 pKa = 3.44RR422 pKa = 11.84YY423 pKa = 10.92DD424 pKa = 3.54EE425 pKa = 4.09YY426 pKa = 11.35RR427 pKa = 11.84RR428 pKa = 11.84AEE430 pKa = 3.91STIAGEE436 pKa = 4.13YY437 pKa = 10.28RR438 pKa = 11.84SVLDD442 pKa = 4.13FWHH445 pKa = 6.73MARR448 pKa = 11.84IFGSSPALNGDD459 pKa = 3.57FVKK462 pKa = 10.67CVPTEE467 pKa = 3.71RR468 pKa = 11.84TFAVPSEE475 pKa = 4.05DD476 pKa = 4.72VLWCMVKK483 pKa = 10.44HH484 pKa = 6.47SIQARR489 pKa = 11.84RR490 pKa = 11.84LVAGVGKK497 pKa = 10.41SFIYY501 pKa = 10.53

Molecular weight: 55.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W789|A0A5Q2W789_9VIRU Uncharacterized protein OS=Microviridae sp. ctYqV29 OX=2656700 PE=4 SV=1
MM1 pKa = 7.08VRR3 pKa = 11.84LGRR6 pKa = 11.84YY7 pKa = 7.71GWIIGGSEE15 pKa = 3.67MRR17 pKa = 11.84RR18 pKa = 11.84GFKK21 pKa = 10.17KK22 pKa = 10.27RR23 pKa = 11.84FARR26 pKa = 11.84GHH28 pKa = 5.15KK29 pKa = 9.49RR30 pKa = 11.84RR31 pKa = 11.84GRR33 pKa = 11.84GVRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84SNYY41 pKa = 7.16QRR43 pKa = 11.84RR44 pKa = 11.84KK45 pKa = 7.33GARR48 pKa = 11.84GVRR51 pKa = 11.84IGFRR55 pKa = 11.84MM56 pKa = 3.56

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1260

56

501

252.0

28.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.492 ± 0.868

1.984 ± 1.172

5.079 ± 0.307

5.079 ± 0.865

2.937 ± 0.457

8.73 ± 0.885

1.508 ± 0.4

3.651 ± 0.298

4.841 ± 1.105

8.413 ± 0.669

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.222 ± 0.271

2.937 ± 0.381

4.841 ± 0.718

4.048 ± 0.693

10.0 ± 2.03

7.54 ± 0.742

4.762 ± 0.686

7.063 ± 0.772

1.905 ± 0.365

3.968 ± 0.565
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski