Leptospirillum ferriphilum (strain ML-04)

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Leptospirillum; Leptospirillum sp. Group III; Leptospirillum ferriphilum

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2450 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9ZDA2|J9ZDA2_LEPFM Adenine phosphoribosyltransferase OS=Leptospirillum ferriphilum (strain ML-04) OX=1048260 GN=apt PE=3 SV=1
MM1 pKa = 7.6HH2 pKa = 7.04EE3 pKa = 4.1TFKK6 pKa = 11.18FLFSCRR12 pKa = 11.84YY13 pKa = 9.76ASVLLSLFALLASGGCNGDD32 pKa = 3.58NLDD35 pKa = 4.45FPSQGGPSATLLQGVAFDD53 pKa = 4.69GPILQGTVSLEE64 pKa = 3.83QYY66 pKa = 10.0PPGSGVLASGTTDD79 pKa = 3.11QNGNFSFTAPMLDD92 pKa = 3.22STAVYY97 pKa = 10.55VLTVSSGKK105 pKa = 8.2TLDD108 pKa = 3.74LATGNTLNLTQGDD121 pKa = 3.8QLMAIGTGQDD131 pKa = 3.43FSGGSLSITPFTSLEE146 pKa = 3.86ASLAEE151 pKa = 3.95HH152 pKa = 7.46FINQGIAMSDD162 pKa = 3.56ALRR165 pKa = 11.84QSDD168 pKa = 4.53DD169 pKa = 3.11LWSGYY174 pKa = 10.95LGFDD178 pKa = 3.68PLKK181 pKa = 10.21TPVANPTLGPTQANSAGLYY200 pKa = 10.24GIALAGLSQMAYY212 pKa = 10.7NIGQTQSLSPGTTNTFGLLIQLQSDD237 pKa = 4.11LSDD240 pKa = 4.37GIFNGLQQGSSTPLSYY256 pKa = 10.85YY257 pKa = 10.54GYY259 pKa = 10.49SLTSDD264 pKa = 3.59TLRR267 pKa = 11.84KK268 pKa = 9.73NLAAGILEE276 pKa = 4.35FLWNTQNKK284 pKa = 10.01SGLTPLTIDD293 pKa = 4.15GFANSLALNTSSLFSEE309 pKa = 4.96TPGASAPDD317 pKa = 3.85PSAPTISVVSPVVGQYY333 pKa = 10.79YY334 pKa = 10.4RR335 pKa = 11.84GTLTIMASASDD346 pKa = 3.83DD347 pKa = 3.84LGIGSFLTTSPNLPLSGGSLDD368 pKa = 3.96QNPLTTSFNTTSVADD383 pKa = 3.91GSYY386 pKa = 11.1NLVFTATDD394 pKa = 3.95YY395 pKa = 11.5AGHH398 pKa = 6.5TASQDD403 pKa = 3.4VQFSVDD409 pKa = 3.61NTPPTISNLSPTNDD423 pKa = 3.12QTLTFCAGITEE434 pKa = 4.68SVTVTGTLTDD444 pKa = 3.6MGSGPNSVGVSEE456 pKa = 4.64IAPTATQLTSSFTISSTNSTTGSFQFTFTVPADD489 pKa = 3.49GCKK492 pKa = 10.03SVTYY496 pKa = 10.32SFSLTGYY503 pKa = 10.6DD504 pKa = 3.46NLFNSSTIPYY514 pKa = 10.12SLTIEE519 pKa = 4.13NN520 pKa = 3.9

Molecular weight: 53.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9Z7F9|J9Z7F9_LEPFM Flagellar biosynthetic protein FliQ OS=Leptospirillum ferriphilum (strain ML-04) OX=1048260 GN=fliQ PE=3 SV=1
MM1 pKa = 7.44RR2 pKa = 11.84HH3 pKa = 6.11APLLLITIYY12 pKa = 10.73RR13 pKa = 11.84SGQTPAPDD21 pKa = 3.5SNRR24 pKa = 11.84RR25 pKa = 11.84ASFFFSVCVRR35 pKa = 11.84FPGGVNDD42 pKa = 4.19NSSTPAQRR50 pKa = 11.84GQLVRR55 pKa = 11.84LDD57 pKa = 3.4RR58 pKa = 11.84KK59 pKa = 9.72GVQKK63 pKa = 10.41IVEE66 pKa = 4.34NGDD69 pKa = 3.53RR70 pKa = 11.84MHH72 pKa = 6.87LQQASLHH79 pKa = 6.95PDD81 pKa = 3.47TAWTSEE87 pKa = 3.84HH88 pKa = 6.23SSEE91 pKa = 4.37RR92 pKa = 11.84FCEE95 pKa = 4.26TEE97 pKa = 3.45PAFAVVVSPDD107 pKa = 2.97VGGIRR112 pKa = 11.84KK113 pKa = 9.16PEE115 pKa = 4.02TVRR118 pKa = 11.84KK119 pKa = 9.98VLFHH123 pKa = 4.76VRR125 pKa = 11.84KK126 pKa = 9.66RR127 pKa = 11.84RR128 pKa = 11.84AHH130 pKa = 5.94GEE132 pKa = 3.79QGRR135 pKa = 11.84SRR137 pKa = 11.84GGIAPPGLVLFSGDD151 pKa = 2.73HH152 pKa = 6.63RR153 pKa = 11.84YY154 pKa = 9.63

Molecular weight: 17.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2450

0

2450

716377

37

1718

292.4

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.355 ± 0.05

0.793 ± 0.018

4.969 ± 0.032

6.501 ± 0.061

4.555 ± 0.039

8.148 ± 0.047

2.226 ± 0.022

5.558 ± 0.04

4.467 ± 0.043

11.135 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.395 ± 0.025

2.896 ± 0.034

5.749 ± 0.035

3.041 ± 0.026

7.292 ± 0.052

7.2 ± 0.044

4.999 ± 0.032

7.022 ± 0.047

1.296 ± 0.022

2.404 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski