Geomicrobium sp. JCM 19039

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillales incertae sedis; Geomicrobium; unclassified Geomicrobium

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4361 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A061PH61|A0A061PH61_9BACL TRAP-type C4-dicarboxylate transport system small permease component OS=Geomicrobium sp. JCM 19039 OX=1460636 GN=JCM19039_3949 PE=4 SV=1
MM1 pKa = 7.4TSKK4 pKa = 10.5KK5 pKa = 10.09HH6 pKa = 5.5LFAGIAGTAVLLAGCTSDD24 pKa = 4.53PGDD27 pKa = 3.58VDD29 pKa = 3.87EE30 pKa = 5.33SAAGSDD36 pKa = 3.83DD37 pKa = 3.48QGEE40 pKa = 4.52VAGGDD45 pKa = 3.71NFVMAVSSDD54 pKa = 2.89IDD56 pKa = 3.5TLDD59 pKa = 3.13PHH61 pKa = 6.88FATDD65 pKa = 3.58VPSSQVTTNIYY76 pKa = 7.06EE77 pKa = 4.33TLVEE81 pKa = 4.29HH82 pKa = 7.26DD83 pKa = 4.23EE84 pKa = 4.2NMEE87 pKa = 4.2VVPALAEE94 pKa = 3.99SFEE97 pKa = 5.11IIDD100 pKa = 3.98DD101 pKa = 4.05LTWEE105 pKa = 4.05FNIRR109 pKa = 11.84EE110 pKa = 4.39GVTFHH115 pKa = 7.71DD116 pKa = 5.1GEE118 pKa = 4.43PLDD121 pKa = 4.37AEE123 pKa = 4.3AVVTSFDD130 pKa = 3.54RR131 pKa = 11.84LFDD134 pKa = 4.38SDD136 pKa = 3.98EE137 pKa = 4.29LKK139 pKa = 10.76PRR141 pKa = 11.84ANMFEE146 pKa = 4.1LVEE149 pKa = 4.34DD150 pKa = 4.58VIAVDD155 pKa = 4.54DD156 pKa = 4.12YY157 pKa = 11.06TVHH160 pKa = 6.98FEE162 pKa = 4.0LSEE165 pKa = 4.14PFASLDD171 pKa = 3.34DD172 pKa = 4.58HH173 pKa = 6.45LTHH176 pKa = 6.63SAAAIISPQAIEE188 pKa = 3.99GHH190 pKa = 6.76DD191 pKa = 3.66NGDD194 pKa = 3.43VLLGTEE200 pKa = 4.84AYY202 pKa = 7.93GTGPFQLEE210 pKa = 3.78HH211 pKa = 6.72WEE213 pKa = 3.91QGNAIDD219 pKa = 4.84LGKK222 pKa = 10.39NEE224 pKa = 5.46DD225 pKa = 3.58YY226 pKa = 10.64WGDD229 pKa = 3.69EE230 pKa = 4.26PQVSTVSFNVVPEE243 pKa = 3.72QSTRR247 pKa = 11.84MGMLDD252 pKa = 3.02QGEE255 pKa = 4.44VHH257 pKa = 7.39FIDD260 pKa = 4.75QIEE263 pKa = 4.22PANAASVDD271 pKa = 4.06NINGASLLSQEE282 pKa = 4.17QLGFQSIAFNTDD294 pKa = 2.65VEE296 pKa = 4.57PFDD299 pKa = 5.46DD300 pKa = 4.74PDD302 pKa = 3.34VRR304 pKa = 11.84RR305 pKa = 11.84ALAMAIDD312 pKa = 4.14RR313 pKa = 11.84EE314 pKa = 4.44AIVEE318 pKa = 4.07GLYY321 pKa = 10.53SGYY324 pKa = 10.71GQVAKK329 pKa = 10.81GPLSPITFGYY339 pKa = 10.29SEE341 pKa = 4.21DD342 pKa = 3.99TEE344 pKa = 5.52AIEE347 pKa = 5.32HH348 pKa = 6.84DD349 pKa = 4.21LDD351 pKa = 4.18AAADD355 pKa = 4.0LLAEE359 pKa = 4.76AGYY362 pKa = 11.01EE363 pKa = 4.08DD364 pKa = 5.09GFSATLYY371 pKa = 10.63TNDD374 pKa = 4.07EE375 pKa = 4.02NHH377 pKa = 6.41SRR379 pKa = 11.84IQLAEE384 pKa = 3.83VAQDD388 pKa = 3.43YY389 pKa = 10.19FGQIGVDD396 pKa = 3.13ISIEE400 pKa = 3.93MMEE403 pKa = 3.82WGTYY407 pKa = 9.64LEE409 pKa = 4.97AMNSGNTEE417 pKa = 3.6IFISGWSTVTADD429 pKa = 4.13ADD431 pKa = 4.05YY432 pKa = 11.75AMHH435 pKa = 7.27PNFHH439 pKa = 6.86SDD441 pKa = 3.18NLGSEE446 pKa = 4.7GNNSFYY452 pKa = 11.4VNEE455 pKa = 4.44EE456 pKa = 3.48VDD458 pKa = 3.71EE459 pKa = 4.71LLDD462 pKa = 3.5EE463 pKa = 4.81ARR465 pKa = 11.84RR466 pKa = 11.84EE467 pKa = 3.91TDD469 pKa = 3.28DD470 pKa = 3.59EE471 pKa = 4.31VRR473 pKa = 11.84EE474 pKa = 4.0DD475 pKa = 4.61LYY477 pKa = 11.63AQAEE481 pKa = 4.15QIIINDD487 pKa = 3.5APQIFTVHH495 pKa = 6.3NDD497 pKa = 3.62YY498 pKa = 9.8ITGISDD504 pKa = 3.61SVDD507 pKa = 2.83GFVLQNNGIYY517 pKa = 10.49DD518 pKa = 4.02FSNVSISGDD527 pKa = 3.16AGGYY531 pKa = 9.06

Molecular weight: 57.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A061PAZ7|A0A061PAZ7_9BACL Ferric iron ABC transporter ATP-binding protein OS=Geomicrobium sp. JCM 19039 OX=1460636 GN=JCM19039_1818 PE=4 SV=1
MM1 pKa = 7.38SKK3 pKa = 8.63KK4 pKa = 8.77TFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.02KK15 pKa = 8.47VHH17 pKa = 5.57GFRR20 pKa = 11.84SRR22 pKa = 11.84MSTKK26 pKa = 10.33NGRR29 pKa = 11.84LVLKK33 pKa = 10.39RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 10.33GRR40 pKa = 11.84KK41 pKa = 8.52VLSAA45 pKa = 4.05

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4361

0

4361

1148845

37

1524

263.4

29.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.694 ± 0.036

0.64 ± 0.01

5.641 ± 0.042

7.707 ± 0.053

4.353 ± 0.035

7.034 ± 0.038

2.476 ± 0.023

6.875 ± 0.036

4.898 ± 0.041

9.517 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.985 ± 0.017

3.867 ± 0.029

3.773 ± 0.027

3.971 ± 0.025

4.781 ± 0.031

6.025 ± 0.024

5.819 ± 0.024

7.557 ± 0.037

1.088 ± 0.015

3.298 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski