Alphacoronavirus BtMs-AlphaCoV/GS2013

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Decacovirus; Rhinolophus ferrumequinum alphacoronavirus HuB-2013

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1WJX0|A0A0U1WJX0_9ALPC Small envelope protein OS=Alphacoronavirus BtMs-AlphaCoV/GS2013 OX=1503290 PE=4 SV=1
MM1 pKa = 7.43FLGLFEE7 pKa = 5.52SSMEE11 pKa = 3.81AAIANGFNIPPNEE24 pKa = 3.98VEE26 pKa = 4.91EE27 pKa = 4.22ILDD30 pKa = 3.8VIKK33 pKa = 10.07PARR36 pKa = 11.84QTISIASFLLSSIFVTWFALYY57 pKa = 10.07KK58 pKa = 10.48ASCFRR63 pKa = 11.84TNCALFALRR72 pKa = 11.84ICTLLLYY79 pKa = 10.09TPILVFFNSYY89 pKa = 9.21IDD91 pKa = 3.77AVVVACSLLLRR102 pKa = 11.84FAYY105 pKa = 10.25LGYY108 pKa = 10.72YY109 pKa = 8.69SYY111 pKa = 11.07KK112 pKa = 9.51YY113 pKa = 10.47KK114 pKa = 10.83SFSFLVLNTSKK125 pKa = 10.89VAFVNGKK132 pKa = 8.15FWYY135 pKa = 9.45YY136 pKa = 10.92DD137 pKa = 3.33EE138 pKa = 4.84SPYY141 pKa = 10.91VVLYY145 pKa = 11.05GGDD148 pKa = 3.1HH149 pKa = 6.05HH150 pKa = 7.05VQFGEE155 pKa = 3.98YY156 pKa = 8.55MIPFADD162 pKa = 3.86SNEE165 pKa = 4.27LYY167 pKa = 10.54VALRR171 pKa = 11.84GTTEE175 pKa = 4.3DD176 pKa = 4.5DD177 pKa = 3.63VPLSRR182 pKa = 11.84KK183 pKa = 9.67VEE185 pKa = 3.94MMNGAFIYY193 pKa = 10.22IFARR197 pKa = 11.84EE198 pKa = 4.11PCVGIVNMSFKK209 pKa = 9.62EE210 pKa = 4.29TQLDD214 pKa = 3.63EE215 pKa = 4.99DD216 pKa = 4.01LTSITLHH223 pKa = 6.13NVDD226 pKa = 3.96ISQQ229 pKa = 3.45

Molecular weight: 26.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1WHF8|A0A0U1WHF8_9ALPC Membrane protein OS=Alphacoronavirus BtMs-AlphaCoV/GS2013 OX=1503290 GN=M PE=3 SV=1
MM1 pKa = 7.6ASVSFQEE8 pKa = 4.47EE9 pKa = 3.91KK10 pKa = 10.28RR11 pKa = 11.84GRR13 pKa = 11.84SGKK16 pKa = 10.09VPLSYY21 pKa = 10.71YY22 pKa = 10.67NPVIVSGDD30 pKa = 3.33KK31 pKa = 10.81PFWKK35 pKa = 10.34VMPNNAVPKK44 pKa = 10.6GKK46 pKa = 10.41GNKK49 pKa = 8.87DD50 pKa = 3.28QQIGYY55 pKa = 8.97WNEE58 pKa = 3.5QPRR61 pKa = 11.84YY62 pKa = 9.28RR63 pKa = 11.84MVRR66 pKa = 11.84GTRR69 pKa = 11.84KK70 pKa = 9.91DD71 pKa = 4.11LPSKK75 pKa = 8.43WHH77 pKa = 6.58FYY79 pKa = 10.71YY80 pKa = 10.91LGTGPHH86 pKa = 6.67AEE88 pKa = 3.88AKK90 pKa = 10.13FRR92 pKa = 11.84TRR94 pKa = 11.84TDD96 pKa = 2.94GVFWVAVQGSKK107 pKa = 9.39TEE109 pKa = 3.99PTGLGTRR116 pKa = 11.84KK117 pKa = 9.96RR118 pKa = 11.84NAEE121 pKa = 3.92LVNPEE126 pKa = 4.35FAIQLPAAIEE136 pKa = 4.03IQEE139 pKa = 4.22NTVSRR144 pKa = 11.84GNSRR148 pKa = 11.84NQSSNRR154 pKa = 11.84DD155 pKa = 3.21RR156 pKa = 11.84SQSGNRR162 pKa = 11.84SQNDD166 pKa = 3.31QKK168 pKa = 11.2QGNQNQKK175 pKa = 10.14SRR177 pKa = 11.84SNSQSRR183 pKa = 11.84KK184 pKa = 9.77GNQNSNDD191 pKa = 3.6SAVDD195 pKa = 2.96IVAAVKK201 pKa = 10.1QALKK205 pKa = 10.54EE206 pKa = 3.9LGVTNEE212 pKa = 4.09SNKK215 pKa = 9.98KK216 pKa = 9.45GKK218 pKa = 10.01NSGTTTPKK226 pKa = 9.52EE227 pKa = 4.05QRR229 pKa = 11.84SKK231 pKa = 11.36SPVRR235 pKa = 11.84SPTVQKK241 pKa = 10.58KK242 pKa = 7.53QLEE245 pKa = 4.23RR246 pKa = 11.84PPWKK250 pKa = 9.82RR251 pKa = 11.84VPNSTEE257 pKa = 4.38DD258 pKa = 3.29VTKK261 pKa = 10.8CFGVRR266 pKa = 11.84DD267 pKa = 3.94TYY269 pKa = 11.62RR270 pKa = 11.84NFGDD274 pKa = 3.78ADD276 pKa = 3.87LVRR279 pKa = 11.84NGIDD283 pKa = 3.11AKK285 pKa = 10.61HH286 pKa = 5.49YY287 pKa = 7.59PQLAEE292 pKa = 4.62LVPTPAAILFGGEE305 pKa = 4.17VVTKK309 pKa = 10.23EE310 pKa = 3.85VGSDD314 pKa = 3.38VEE316 pKa = 3.66ITYY319 pKa = 9.76IYY321 pKa = 10.16KK322 pKa = 9.86MKK324 pKa = 10.6VPKK327 pKa = 9.43TDD329 pKa = 3.07KK330 pKa = 10.98NLAAFLKK337 pKa = 10.3QVSAYY342 pKa = 9.25SQPSQAAEE350 pKa = 4.21VPSQLNPAAVVFQPLAEE367 pKa = 4.66DD368 pKa = 3.79EE369 pKa = 4.17QVEE372 pKa = 5.14IIDD375 pKa = 3.63QVYY378 pKa = 10.91GSFDD382 pKa = 3.02AA383 pKa = 5.67

Molecular weight: 42.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

8899

65

6740

1483.2

165.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.54 ± 0.199

3.135 ± 0.805

5.113 ± 0.544

4.495 ± 0.269

5.652 ± 0.491

6.461 ± 0.46

1.697 ± 0.322

5.248 ± 0.639

6.046 ± 0.877

8.158 ± 0.681

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.068 ± 0.287

6.124 ± 0.453

3.528 ± 0.586

3.18 ± 0.95

3.225 ± 0.655

7.394 ± 0.5

5.967 ± 0.487

10.024 ± 0.324

1.18 ± 0.255

4.765 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski