Thermohalobacter berrensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Thermohalobacteraceae; Thermohalobacter

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2592 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A419T3K5|A0A419T3K5_9CLOT Glycogen synthase OS=Thermohalobacter berrensis OX=99594 GN=glgA PE=3 SV=1
MM1 pKa = 7.68SEE3 pKa = 4.77VKK5 pKa = 10.41FCEE8 pKa = 3.82NNFAQGVGDD17 pKa = 3.62VKK19 pKa = 11.16LKK21 pKa = 10.97VEE23 pKa = 4.14NEE25 pKa = 3.63MDD27 pKa = 3.56NVNVEE32 pKa = 4.15VEE34 pKa = 4.2PCLGYY39 pKa = 11.0CGDD42 pKa = 4.04CAMGPFALVNDD53 pKa = 4.36EE54 pKa = 5.55FIQADD59 pKa = 4.35TPEE62 pKa = 4.01EE63 pKa = 4.33LYY65 pKa = 11.15DD66 pKa = 4.31KK67 pKa = 10.73IKK69 pKa = 10.91QEE71 pKa = 4.37LEE73 pKa = 3.79

Molecular weight: 8.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A419T2J3|A0A419T2J3_9CLOT (2Fe-2S)-binding protein OS=Thermohalobacter berrensis OX=99594 GN=BET03_12270 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.08GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 9.82SGRR28 pKa = 11.84RR29 pKa = 11.84ILKK32 pKa = 8.48NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.91GRR39 pKa = 11.84KK40 pKa = 9.0RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2592

0

2592

781713

37

2065

301.6

34.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.491 ± 0.057

0.88 ± 0.017

5.5 ± 0.048

7.691 ± 0.059

4.113 ± 0.037

6.653 ± 0.047

1.387 ± 0.018

10.418 ± 0.057

9.932 ± 0.058

9.2 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.019

5.935 ± 0.039

3.126 ± 0.027

2.17 ± 0.019

3.733 ± 0.028

5.375 ± 0.035

4.732 ± 0.029

6.685 ± 0.038

0.64 ± 0.014

3.938 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski