Staphylococcus phage phiMR11

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9CR76|A9CR76_BPMR1 Putative transcriptional regulator OS=Staphylococcus phage phiMR11 OX=379501 GN=orf23 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.45VEE20 pKa = 3.82YY21 pKa = 10.92QHH23 pKa = 6.88FDD25 pKa = 3.86DD26 pKa = 5.96VDD28 pKa = 3.7DD29 pKa = 5.1EE30 pKa = 5.37KK31 pKa = 11.65DD32 pKa = 3.57VLAKK36 pKa = 10.68RR37 pKa = 11.84LDD39 pKa = 4.12DD40 pKa = 5.44NPDD43 pKa = 3.35EE44 pKa = 4.2LLKK47 pKa = 10.77YY48 pKa = 10.55ANITIRR54 pKa = 11.84HH55 pKa = 5.96AYY57 pKa = 9.38IEE59 pKa = 4.35VEE61 pKa = 4.03

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9CR91|A9CR91_BPMR1 Uncharacterized protein OS=Staphylococcus phage phiMR11 OX=379501 GN=orf33 PE=4 SV=1
MM1 pKa = 7.76SYY3 pKa = 10.53IITLVLILVFIVIFNNLLNRR23 pKa = 11.84YY24 pKa = 6.94MVLYY28 pKa = 10.65KK29 pKa = 10.58EE30 pKa = 5.29LDD32 pKa = 3.45LFTCRR37 pKa = 11.84IGMLLVLIVIVDD49 pKa = 4.1FAKK52 pKa = 9.87QQNMLATLSVLLILLFVEE70 pKa = 4.58KK71 pKa = 10.61LRR73 pKa = 11.84IIQRR77 pKa = 11.84SDD79 pKa = 2.57KK80 pKa = 10.82KK81 pKa = 11.06

Molecular weight: 9.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13487

46

989

201.3

22.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.902 ± 0.386

0.512 ± 0.085

6.384 ± 0.283

7.459 ± 0.425

4.056 ± 0.179

6.021 ± 0.353

1.735 ± 0.154

7.481 ± 0.249

9.075 ± 0.405

7.548 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.197

6.495 ± 0.3

2.825 ± 0.191

4.197 ± 0.206

3.989 ± 0.233

5.761 ± 0.27

6.236 ± 0.292

6.199 ± 0.311

1.216 ± 0.163

4.308 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski