Burkholderia phage KS5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Kisquinquevirus; Burkholderia virus KS5

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5E3Q1|E5E3Q1_9CAUD Gp16 OS=Burkholderia phage KS5 OX=910473 GN=16 PE=4 SV=1
MM1 pKa = 7.29QSKK4 pKa = 9.82QSAVITLDD12 pKa = 3.45TPIKK16 pKa = 10.41RR17 pKa = 11.84GEE19 pKa = 4.01QEE21 pKa = 3.57ITAVTLNKK29 pKa = 9.69PLAGALRR36 pKa = 11.84GVALTDD42 pKa = 3.37VLQLDD47 pKa = 4.73VIALSKK53 pKa = 10.14VLPRR57 pKa = 11.84ISDD60 pKa = 3.78PVLTTQDD67 pKa = 3.56VLRR70 pKa = 11.84LDD72 pKa = 4.16PADD75 pKa = 3.85LVQLGTEE82 pKa = 4.06VAGFLGAEE90 pKa = 4.1LVEE93 pKa = 4.64GGRR96 pKa = 11.84LPRR99 pKa = 11.84TVDD102 pKa = 4.06DD103 pKa = 4.01VMADD107 pKa = 3.14IALVFHH113 pKa = 7.04WSPDD117 pKa = 2.82AMAAMPLSEE126 pKa = 4.37LMDD129 pKa = 3.05WRR131 pKa = 11.84EE132 pKa = 3.96RR133 pKa = 11.84ARR135 pKa = 11.84EE136 pKa = 3.67RR137 pKa = 11.84YY138 pKa = 9.28EE139 pKa = 4.51RR140 pKa = 11.84GDD142 pKa = 3.4EE143 pKa = 3.96

Molecular weight: 15.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5E3Q6|E5E3Q6_9CAUD Gp22 OS=Burkholderia phage KS5 OX=910473 GN=22 PE=3 SV=1
MM1 pKa = 7.47QDD3 pKa = 3.12IRR5 pKa = 11.84CASCNRR11 pKa = 11.84KK12 pKa = 9.61LGAGEE17 pKa = 4.21YY18 pKa = 9.81IRR20 pKa = 11.84LTIKK24 pKa = 10.57CPRR27 pKa = 11.84CRR29 pKa = 11.84AMNVLRR35 pKa = 11.84AEE37 pKa = 4.27RR38 pKa = 11.84PLPAGHH44 pKa = 7.01RR45 pKa = 11.84ASDD48 pKa = 3.63TRR50 pKa = 11.84EE51 pKa = 4.11SPHH54 pKa = 6.06ATPHH58 pKa = 5.73LRR60 pKa = 3.59

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

10931

60

931

237.6

26.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.268 ± 0.446

0.823 ± 0.123

6.276 ± 0.319

5.498 ± 0.274

3.284 ± 0.188

7.758 ± 0.497

2.04 ± 0.161

4.501 ± 0.203

4.272 ± 0.29

9.368 ± 0.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.187

2.854 ± 0.18

4.647 ± 0.265

3.668 ± 0.177

8.115 ± 0.331

5.324 ± 0.272

6.175 ± 0.361

7.062 ± 0.314

1.381 ± 0.149

2.488 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski