Candidatus Rhodobacter lobularis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobacter

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4763 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0J9E1N7|A0A0J9E1N7_9RHOB Sarcosine oxidase beta subunit OS=Candidatus Rhodobacter lobularis OX=1675527 GN=AIOL_001767 PE=4 SV=1
MM1 pKa = 7.82SIFSKK6 pKa = 10.83LNRR9 pKa = 11.84ALSAIFFVAVAAAGSPSWAQPAFTVGFTPNSIAPSGTTRR48 pKa = 11.84MIFTIDD54 pKa = 3.46NSSGGAVTSMDD65 pKa = 3.65FTNALPAGITVAVAPNAFTSCGGASASSATVTAMAGGSSIAMADD109 pKa = 3.62GQLAPGATCTVEE121 pKa = 4.45ANVTATATATNGPATLNTSNGSSASASVGLTVDD154 pKa = 3.8AAATAVGKK162 pKa = 10.54AFAPTTIGIGGVSRR176 pKa = 11.84LTLTVDD182 pKa = 3.55NSAGANAQNFTLTDD196 pKa = 3.64NLPAGMVLADD206 pKa = 4.47PPNTATTCPVAQSQTLTGVGGGSILEE232 pKa = 4.16LQSTGGLVFTPSFMVVNALPAGTVCTVSADD262 pKa = 3.55VTASVPGSLQNVTEE276 pKa = 4.34PFLDD280 pKa = 3.91SGSAAATLTVTGAPAGAPQLTKK302 pKa = 10.77EE303 pKa = 4.24FTDD306 pKa = 4.14DD307 pKa = 3.28PVAAGGSATLEE318 pKa = 3.78FEE320 pKa = 3.78ILNRR324 pKa = 11.84SRR326 pKa = 11.84SDD328 pKa = 3.08AASNIAFTDD337 pKa = 3.53DD338 pKa = 2.98MSALFGALAGTVTAIGTNTCGGTPSGVGTSSLSYY372 pKa = 10.75SGGTVGAGLRR382 pKa = 11.84CNITVTISVPAGVSTGSYY400 pKa = 9.24TNTSTAVTADD410 pKa = 3.48VGGMSLTGNAATANLSVLAALDD432 pKa = 4.11LPPVLSFTTPDD443 pKa = 3.23GNAGGVSTWSFTLSNAASSALTDD466 pKa = 3.42GTFDD470 pKa = 3.49VQMNPPLPFPTNASLPPMPDD490 pKa = 3.29PPCGVGSSLSLAFLDD505 pKa = 4.1TDD507 pKa = 3.68QQALRR512 pKa = 11.84LTGASLATSGAAGDD526 pKa = 3.88SCTFDD531 pKa = 3.27VDD533 pKa = 3.37IGLNSDD539 pKa = 5.14LPPLQYY545 pKa = 11.04NFTSGAPSGTVNGATRR561 pKa = 11.84TGTPASSSMTVGTNLNLSFAKK582 pKa = 10.45SFLATAVPGGTVDD595 pKa = 3.94LEE597 pKa = 4.28FVITSSGDD605 pKa = 3.46STDD608 pKa = 2.91ATAISFTDD616 pKa = 5.0DD617 pKa = 3.09INAMLAGTSLNAVISNEE634 pKa = 4.01CGGNLTGTTNLSYY647 pKa = 10.57TGGSLTGGTSCAIVTRR663 pKa = 11.84LDD665 pKa = 3.43VPAAAAIGTYY675 pKa = 10.32TNTTSALSATPSGSSTVEE693 pKa = 3.91TFGTASADD701 pKa = 3.47LTVAGLTFEE710 pKa = 5.78KK711 pKa = 10.68EE712 pKa = 4.04FLTNTVLAGDD722 pKa = 3.84TTTLRR727 pKa = 11.84FTISNVHH734 pKa = 5.72PTADD738 pKa = 3.23ATITSFTDD746 pKa = 3.15NLQQNLTGLAATGGPSGNTCGGTLSGTTFLIYY778 pKa = 10.26TGGSVTSGMSCTIDD792 pKa = 3.52VEE794 pKa = 4.53VLVPVGTADD803 pKa = 3.25GSYY806 pKa = 11.42GNTTSTLSATQGGGPVVVDD825 pKa = 4.0PATDD829 pKa = 3.14ILNVQGLQIEE839 pKa = 4.43LSKK842 pKa = 11.32AFTDD846 pKa = 4.42DD847 pKa = 3.45PVAPGDD853 pKa = 3.64NATLEE858 pKa = 4.41FTLSNLSSTEE868 pKa = 4.12AISNIAFTDD877 pKa = 3.5DD878 pKa = 3.76LAAMLPGTTFASVAGNDD895 pKa = 3.41CGATVGGTGTSTISVSGTSLAASATCTITVAVTVPGGAVDD935 pKa = 3.52NTYY938 pKa = 10.96TNTTSSVTGDD948 pKa = 2.79IGGLAVTGDD957 pKa = 3.84PATAQLSVSSILPLAFSKK975 pKa = 10.94SFSGSVASGEE985 pKa = 4.37SGTLTFTLSNPNTTALSDD1003 pKa = 4.2LGFTDD1008 pKa = 5.29DD1009 pKa = 4.78LNAMLTGVVATSLPSAPCGLGSSITGTSSLTASGFSLDD1047 pKa = 4.27ASGGSCTFDD1056 pKa = 2.78VGFSVPVTATGATYY1070 pKa = 11.21LNTTSEE1076 pKa = 4.37LVSFGLSLAPPATANLIVTDD1096 pKa = 4.52PVADD1100 pKa = 4.37LSLAIADD1107 pKa = 4.24SPDD1110 pKa = 3.59PVTPGQNLVYY1120 pKa = 10.12TVTASNAGPQTAQSVAVNLPVPTGTTLVSTTGCAEE1155 pKa = 4.41DD1156 pKa = 3.86PSGAATCSLGNIASGGNAQYY1176 pKa = 10.53TLTVTVDD1183 pKa = 3.0AATSGTVSASGTVSSSTSDD1202 pKa = 3.19PVAGNNTATASTSAPATADD1221 pKa = 3.12ISITKK1226 pKa = 10.16SDD1228 pKa = 3.63GVTSANSGGTTTYY1241 pKa = 10.78SIVVANAGPSADD1253 pKa = 3.74PAVSVADD1260 pKa = 3.92TFAADD1265 pKa = 4.66LGCATTSVAAGGATGNTAAFSGDD1288 pKa = 3.07IAEE1291 pKa = 4.46TLSMPASSSVTYY1303 pKa = 9.09TATCDD1308 pKa = 3.17IAITATGTLSNTATATASVLDD1329 pKa = 4.37PNATNNSATDD1339 pKa = 3.17SDD1341 pKa = 4.34TALVAPVAMGFSKK1354 pKa = 10.76AFSPATIDD1362 pKa = 3.19QGANTTLTFTIDD1374 pKa = 3.04NTANAVAADD1383 pKa = 3.92NLNFTDD1389 pKa = 5.15GFPLGVDD1396 pKa = 3.92LSVAAPVVSSSTCGGTFNPVAGGGNLVFSGGSVAAGATCTVSVLLNARR1444 pKa = 11.84GSGTATNTSSQLGSFFPTPSAAATADD1470 pKa = 3.23ITVNPAVAPTFAKK1483 pKa = 10.65AFGAATIEE1491 pKa = 4.06QGQTTTATFTINNSANSIEE1510 pKa = 4.14ASSLQFSDD1518 pKa = 3.71TLPVEE1523 pKa = 4.44LVVAATPNASTTCTGGTITATAGSGSILYY1552 pKa = 10.56SGGEE1556 pKa = 4.03VAAGATCTVTVTVDD1570 pKa = 3.32SLGSGSIANPSLNLTSSLPTATAAATPLTINPATAPGVAKK1610 pKa = 9.81TFTPSAITQGEE1621 pKa = 4.31TSALSITIDD1630 pKa = 3.12NSANFLEE1637 pKa = 4.45MTGVAFTDD1645 pKa = 4.08AFPSGMVVGPTPGASNTCGGTFSASAGSGTISLSGGTVAAGATCSVGVTVRR1696 pKa = 11.84GNAAGTLTNPAFDD1709 pKa = 3.67VTSNIATGTAAATDD1723 pKa = 4.08LAVNPAAAPGFAKK1736 pKa = 10.65AFGAGTINQGGTVVATVSVDD1756 pKa = 3.39NSGNAADD1763 pKa = 4.19LSGMGFTDD1771 pKa = 4.03TLPAGLVIAATPAEE1785 pKa = 4.34TNTCGGSLTANAGASTFALSGGALAGGGTCAVSVTLRR1822 pKa = 11.84ALEE1825 pKa = 4.51AGSLTNPMFDD1835 pKa = 3.3LTSNIATATAASAILTVNAASAPVVAKK1862 pKa = 10.27GFAPAIINQGQTTTATVSIDD1882 pKa = 3.07NAANLIEE1889 pKa = 4.25ATGVAFTDD1897 pKa = 3.85ALPSGLVVAPTPAGSNTCGGTLTANAGASSFALSGGTIAEE1937 pKa = 4.42GASCQVTVALRR1948 pKa = 11.84ALDD1951 pKa = 4.43GGTLTNPAFDD1961 pKa = 3.88VTSSIATGTVAATDD1975 pKa = 3.76LTVNAAAAPGFAKK1988 pKa = 10.46AFSVSTLDD1996 pKa = 3.5QGQSLTATVTIDD2008 pKa = 3.32NAPNAIEE2015 pKa = 4.15ASNLAFTDD2023 pKa = 3.75ALPAGLVIGTASGGGSLISTTCGGTASATTGASSFSLSGGTLAEE2067 pKa = 4.56GASCTVVVVLRR2078 pKa = 11.84AVGAGTLTNPAMDD2091 pKa = 4.23LTSSIATATSAPVSLTANAAAAPVVTKK2118 pKa = 9.82VFAPATIEE2126 pKa = 3.89QGQTTTATITIDD2138 pKa = 3.15NAGNSIEE2145 pKa = 4.08AASLAFTDD2153 pKa = 4.09TLPAGLQVAAVPNEE2167 pKa = 3.97SNTCGGTLTAAAGTGAVSLSGGTLAEE2193 pKa = 4.49GASCAIAVDD2202 pKa = 4.69LRR2204 pKa = 11.84ATLPGTLTNPGFDD2217 pKa = 3.59VTSSIATGSAAATDD2231 pKa = 3.92LTVNAAAAMGFAKK2244 pKa = 10.4AFAPATIDD2252 pKa = 3.53LGSSSTLTFTIDD2264 pKa = 2.95NSANAIEE2271 pKa = 4.29AASMAFTDD2279 pKa = 4.28SFPAGLIVSSAPAATNTCGGTFTATALSGLVSLSGGTLAAGTTCTLSVQVQALTTGSLDD2338 pKa = 3.56NTSSEE2343 pKa = 4.36LTSDD2347 pKa = 4.37LPSASPVATASLTVNAVPITSAASFNPPIIEE2378 pKa = 3.98LFEE2381 pKa = 4.04LTEE2384 pKa = 4.78LVFLFQNDD2392 pKa = 3.17ADD2394 pKa = 3.98IDD2396 pKa = 3.94QILNFIQNLPAGLNVGGAATSRR2418 pKa = 11.84ANAMAGTNVSTTCSGGTVTAVEE2440 pKa = 5.06GSTQVQISGATVPANGSCEE2459 pKa = 4.13LKK2461 pKa = 11.15VNVIANAIGDD2471 pKa = 4.0FSNLVGDD2478 pKa = 4.33ADD2480 pKa = 4.2LVVNTIARR2488 pKa = 11.84AFVTYY2493 pKa = 10.6VVTGSDD2499 pKa = 2.95GSYY2502 pKa = 10.57AFSSSATEE2510 pKa = 3.9LNATVAVAGGTGTQGPLRR2528 pKa = 11.84SSEE2531 pKa = 3.65GSYY2534 pKa = 10.93SVAIAPPTGVAFDD2547 pKa = 4.9SGVCDD2552 pKa = 5.29DD2553 pKa = 5.04GDD2555 pKa = 4.22SSVDD2559 pKa = 3.63LANNAIALTLSPLEE2573 pKa = 4.34SVTCTFTALSPVQQTVDD2590 pKa = 3.88VINQFLTKK2598 pKa = 10.21RR2599 pKa = 11.84ADD2601 pKa = 4.26LILSTEE2607 pKa = 4.21PNLGARR2613 pKa = 11.84GRR2615 pKa = 11.84DD2616 pKa = 3.05RR2617 pKa = 11.84LGRR2620 pKa = 11.84GFGNASALSFANGDD2634 pKa = 4.04LKK2636 pKa = 11.71AMLPFTATMQPGNYY2650 pKa = 9.16SFKK2653 pKa = 11.03SSLLQVRR2660 pKa = 11.84QAAASVQLAHH2670 pKa = 7.21GSTKK2674 pKa = 10.67DD2675 pKa = 3.37AIYY2678 pKa = 10.31VPNYY2682 pKa = 10.41RR2683 pKa = 11.84FDD2685 pKa = 3.39AWFEE2689 pKa = 3.89AHH2691 pKa = 6.81HH2692 pKa = 6.64KK2693 pKa = 10.77EE2694 pKa = 4.86FDD2696 pKa = 3.18TGTNGTGHH2704 pKa = 6.04FTAAHH2709 pKa = 6.39IGADD2713 pKa = 3.43YY2714 pKa = 11.18VLNEE2718 pKa = 4.01NLLIGAMVSFDD2729 pKa = 4.05SMEE2732 pKa = 5.42DD2733 pKa = 3.24DD2734 pKa = 3.83TAISSASGSGWLIGPYY2750 pKa = 7.9MTAKK2754 pKa = 10.47LAPNLYY2760 pKa = 10.08FDD2762 pKa = 4.57ARR2764 pKa = 11.84LAGGASNNKK2773 pKa = 8.6VSPFNTYY2780 pKa = 9.01TDD2782 pKa = 3.41SFDD2785 pKa = 3.5TRR2787 pKa = 11.84RR2788 pKa = 11.84LLASASLSGDD2798 pKa = 3.5FQQGNWTISPTASLSYY2814 pKa = 10.56YY2815 pKa = 10.5RR2816 pKa = 11.84EE2817 pKa = 4.19TQDD2820 pKa = 4.61SYY2822 pKa = 11.69IDD2824 pKa = 3.55STGASIPSQTVEE2836 pKa = 4.41LGQLKK2841 pKa = 10.17IGPTFTGRR2849 pKa = 11.84FLGDD2853 pKa = 3.76DD2854 pKa = 4.05GEE2856 pKa = 5.58IIAPYY2861 pKa = 9.88FSVDD2865 pKa = 3.37AIYY2868 pKa = 11.21NLGQTSGVTVTNPDD2882 pKa = 3.47TASTSGWRR2890 pKa = 11.84GRR2892 pKa = 11.84LKK2894 pKa = 10.92AGVTVTEE2901 pKa = 4.23EE2902 pKa = 3.87NGTKK2906 pKa = 10.39LGIGATYY2913 pKa = 10.74DD2914 pKa = 3.27GLFRR2918 pKa = 11.84SDD2920 pKa = 3.01FDD2922 pKa = 3.17AWGLSFEE2929 pKa = 5.52LEE2931 pKa = 4.07IPLQKK2936 pKa = 9.32PTARR2940 pKa = 4.09

Molecular weight:
287.35 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0J9H4A4|A0A0J9H4A4_9RHOB Membrane protein OS=Candidatus Rhodobacter lobularis OX=1675527 GN=AIOL_000659 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight:
5.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4763

0

4763

1428562

37

2940

299.9

32.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.623 ± 0.051

0.942 ± 0.01

5.927 ± 0.031

5.974 ± 0.034

3.832 ± 0.022

8.847 ± 0.039

2.074 ± 0.018

5.071 ± 0.027

3.098 ± 0.029

9.885 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.777 ± 0.017

2.673 ± 0.021

5.053 ± 0.026

3.367 ± 0.021

6.51 ± 0.037

5.076 ± 0.03

5.25 ± 0.029

7.257 ± 0.032

1.481 ± 0.014

2.283 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski