Halomonas phage phiHAP-1 (isolate -/Gulf of Mexico/-/2001) (Bacteriophage phiHAP-1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Hapunavirus; Halomonas virus HAP1

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0ZSH7|B0ZSH7_BPHA1 Uncharacterized protein OS=Halomonas phage phiHAP-1 (isolate -/Gulf of Mexico/-/2001) OX=1283337 GN=HAPgp29 PE=4 SV=1
MM1 pKa = 7.0VCSSAGGDD9 pKa = 3.33ACHH12 pKa = 6.43GQQTEE17 pKa = 4.09DD18 pKa = 3.32APMIIHH24 pKa = 6.73TVTSYY29 pKa = 11.46AFQTHH34 pKa = 5.22VVRR37 pKa = 11.84DD38 pKa = 3.71TDD40 pKa = 3.96GEE42 pKa = 4.18IMIGIDD48 pKa = 3.78GPRR51 pKa = 11.84GPILVEE57 pKa = 3.31IHH59 pKa = 6.37SADD62 pKa = 3.22EE63 pKa = 4.17STIEE67 pKa = 4.22RR68 pKa = 11.84LLTHH72 pKa = 6.92GDD74 pKa = 3.72EE75 pKa = 4.64EE76 pKa = 5.04DD77 pKa = 3.88LSDD80 pKa = 3.52MCSATIHH87 pKa = 7.05DD88 pKa = 5.96FYY90 pKa = 10.99TQIGRR95 pKa = 11.84CFPGDD100 pKa = 3.82TFSHH104 pKa = 6.77FADD107 pKa = 3.71APGEE111 pKa = 3.96IGEE114 pKa = 4.53LVTRR118 pKa = 11.84PRR120 pKa = 11.84TAKK123 pKa = 10.1EE124 pKa = 3.48IQQARR129 pKa = 11.84EE130 pKa = 3.87SIAYY134 pKa = 9.36RR135 pKa = 11.84DD136 pKa = 4.12LIEE139 pKa = 5.34GGLSEE144 pKa = 5.4QEE146 pKa = 3.74IQALIVV152 pKa = 3.3

Molecular weight: 16.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0ZSI0|B0ZSI0_BPHA1 Uncharacterized protein OS=Halomonas phage phiHAP-1 (isolate -/Gulf of Mexico/-/2001) OX=1283337 GN=HAPgp32 PE=4 SV=1
MM1 pKa = 7.46EE2 pKa = 5.54SYY4 pKa = 11.04VEE6 pKa = 4.05ARR8 pKa = 11.84DD9 pKa = 3.57YY10 pKa = 11.52HH11 pKa = 6.46NPPDD15 pKa = 3.97HH16 pKa = 6.04AAKK19 pKa = 10.43RR20 pKa = 11.84AGASRR25 pKa = 11.84AGRR28 pKa = 11.84TLCRR32 pKa = 11.84RR33 pKa = 11.84EE34 pKa = 3.77HH35 pKa = 6.44HH36 pKa = 6.93PRR38 pKa = 11.84LCIPQPAEE46 pKa = 3.78AAGGGAMNTTISRR59 pKa = 11.84VEE61 pKa = 3.9KK62 pKa = 9.94TLEE65 pKa = 3.8RR66 pKa = 11.84QQARR70 pKa = 11.84IQALEE75 pKa = 3.85AALAPFAKK83 pKa = 9.69AWEE86 pKa = 4.23FRR88 pKa = 11.84RR89 pKa = 11.84PSGNGDD95 pKa = 2.91AVARR99 pKa = 11.84RR100 pKa = 11.84LRR102 pKa = 11.84QKK104 pKa = 10.22RR105 pKa = 11.84CLDD108 pKa = 3.25GFYY111 pKa = 9.97TARR114 pKa = 11.84EE115 pKa = 4.11LGSEE119 pKa = 4.08RR120 pKa = 11.84TLTLTGRR127 pKa = 11.84HH128 pKa = 6.01LKK130 pKa = 10.04DD131 pKa = 2.95AHH133 pKa = 6.81DD134 pKa = 4.31ALKK137 pKa = 10.78AKK139 pKa = 9.98PP140 pKa = 3.42

Molecular weight: 15.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11479

65

1347

249.5

27.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.837 ± 0.684

0.923 ± 0.154

6.281 ± 0.247

7.588 ± 0.275

2.927 ± 0.167

7.509 ± 0.427

2.248 ± 0.308

4.608 ± 0.218

3.807 ± 0.351

8.459 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.448 ± 0.164

3.241 ± 0.195

4.408 ± 0.322

4.295 ± 0.218

8.023 ± 0.303

5.645 ± 0.241

5.419 ± 0.287

6.926 ± 0.281

1.751 ± 0.197

2.648 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski