Spiroplasma clarkii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1324 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8KK26|A0A2K8KK26_9MOLU Uncharacterized protein OS=Spiroplasma clarkii OX=2139 GN=SCLAR_v1c04550 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 9.08KK3 pKa = 8.24TLSLLGSFAFVGSVSLAAANTVSCFWWKK31 pKa = 8.33NTAVPPKK38 pKa = 10.24SYY40 pKa = 10.92LKK42 pKa = 10.5DD43 pKa = 3.73YY44 pKa = 10.71ISKK47 pKa = 8.97TEE49 pKa = 3.97LGEE52 pKa = 3.93FSGVDD57 pKa = 3.43DD58 pKa = 4.88LPSLEE63 pKa = 5.19EE64 pKa = 3.81IVQKK68 pKa = 10.71LWEE71 pKa = 4.39ANYY74 pKa = 11.04ADD76 pKa = 3.6VDD78 pKa = 3.7EE79 pKa = 5.28TYY81 pKa = 10.92LEE83 pKa = 4.02NGIFINLYY91 pKa = 9.77FVEE94 pKa = 5.75DD95 pKa = 4.24PTTMQAIISVPTSIWSYY112 pKa = 10.33DD113 pKa = 3.17AGNITVTYY121 pKa = 9.65IYY123 pKa = 10.84SKK125 pKa = 9.0YY126 pKa = 9.93QEE128 pKa = 4.38SEE130 pKa = 4.2SLLSSFF136 pKa = 5.31

Molecular weight: 15.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8KJI4|A0A2K8KJI4_9MOLU 30S ribosomal protein S10 OS=Spiroplasma clarkii OX=2139 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.42NGRR28 pKa = 11.84KK29 pKa = 8.57VLKK32 pKa = 10.26ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.61GRR39 pKa = 11.84VRR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1324

0

1324

452743

31

2209

342.0

38.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.112 ± 0.072

0.787 ± 0.017

5.409 ± 0.057

6.535 ± 0.071

5.483 ± 0.058

5.27 ± 0.07

1.337 ± 0.026

9.045 ± 0.067

9.062 ± 0.064

9.538 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.174 ± 0.029

6.712 ± 0.078

2.712 ± 0.039

3.63 ± 0.043

2.779 ± 0.038

6.214 ± 0.051

5.645 ± 0.054

6.361 ± 0.05

1.138 ± 0.026

4.057 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski