Prunus geminivirus A

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Grablovirus; Prunus latent virus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L1GUQ4|A0A2L1GUQ4_9GEMI C3 Protein OS=Prunus geminivirus A OX=2022321 PE=4 SV=1
MM1 pKa = 7.11MFLVVVGMMLSVIAYY16 pKa = 7.56IWRR19 pKa = 11.84VLLLPDD25 pKa = 3.81ILSGYY30 pKa = 10.05ILMGLLISIMVLFFHH45 pKa = 7.7RR46 pKa = 11.84PRR48 pKa = 11.84CLIWRR53 pKa = 11.84VYY55 pKa = 10.71DD56 pKa = 3.48QVSEE60 pKa = 4.09VGLYY64 pKa = 10.39DD65 pKa = 4.58GYY67 pKa = 11.54DD68 pKa = 3.53DD69 pKa = 4.03VVKK72 pKa = 10.54DD73 pKa = 3.81IPIDD77 pKa = 3.98GDD79 pKa = 3.65DD80 pKa = 4.36DD81 pKa = 4.08EE82 pKa = 7.19SYY84 pKa = 10.26MVSDD88 pKa = 4.91EE89 pKa = 4.09YY90 pKa = 11.85AEE92 pKa = 3.95FLEE95 pKa = 4.26TLKK98 pKa = 10.55RR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84SEE103 pKa = 4.0RR104 pKa = 11.84GGMTSNLKK112 pKa = 9.88RR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84VMVDD119 pKa = 2.91PDD121 pKa = 3.44EE122 pKa = 5.79DD123 pKa = 3.68LVNRR127 pKa = 11.84LKK129 pKa = 10.76EE130 pKa = 3.95YY131 pKa = 10.63LGEE134 pKa = 4.34DD135 pKa = 3.32EE136 pKa = 4.35YY137 pKa = 11.76DD138 pKa = 2.99RR139 pKa = 11.84HH140 pKa = 6.56RR141 pKa = 11.84RR142 pKa = 11.84RR143 pKa = 11.84LDD145 pKa = 3.28LEE147 pKa = 4.68LGSGQHH153 pKa = 6.27RR154 pKa = 11.84PDD156 pKa = 3.31SRR158 pKa = 11.84CVVMM162 pKa = 5.93

Molecular weight: 19.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L1GUS0|A0A2L1GUS0_9GEMI CP/V1 protein OS=Prunus geminivirus A OX=2022321 GN=CP PE=4 SV=1
MM1 pKa = 6.78VLKK4 pKa = 10.39RR5 pKa = 11.84RR6 pKa = 11.84PSRR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 9.24RR12 pKa = 11.84RR13 pKa = 11.84ATKK16 pKa = 9.95KK17 pKa = 10.24RR18 pKa = 11.84SGRR21 pKa = 11.84SSQRR25 pKa = 11.84KK26 pKa = 7.87RR27 pKa = 11.84RR28 pKa = 11.84ARR30 pKa = 11.84PRR32 pKa = 11.84SRR34 pKa = 11.84PCQFSFHH41 pKa = 6.31GNAFGGTPTLFFLTPIALGNGAEE64 pKa = 4.26DD65 pKa = 3.4RR66 pKa = 11.84TGSVLTVSSMYY77 pKa = 10.75LKK79 pKa = 10.74GVVVPTDD86 pKa = 3.17NVTDD90 pKa = 4.27GLHH93 pKa = 7.49DD94 pKa = 3.64IYY96 pKa = 11.1FWIILDD102 pKa = 4.21RR103 pKa = 11.84FPTGTDD109 pKa = 3.28PAVSDD114 pKa = 3.93IFTGSDD120 pKa = 2.79TGGSMIEE127 pKa = 3.9TLTRR131 pKa = 11.84NRR133 pKa = 11.84LNRR136 pKa = 11.84KK137 pKa = 8.14RR138 pKa = 11.84FRR140 pKa = 11.84ILGSKK145 pKa = 10.12KK146 pKa = 10.37LVIGVNRR153 pKa = 11.84KK154 pKa = 7.78PQEE157 pKa = 4.33SLPHH161 pKa = 5.12TRR163 pKa = 11.84AAFNIFQRR171 pKa = 11.84RR172 pKa = 11.84RR173 pKa = 11.84LVVAFKK179 pKa = 11.03NDD181 pKa = 3.04VSGGGRR187 pKa = 11.84NDD189 pKa = 3.11VEE191 pKa = 4.26RR192 pKa = 11.84NRR194 pKa = 11.84IYY196 pKa = 9.69MACASATGHH205 pKa = 6.1SFRR208 pKa = 11.84LYY210 pKa = 11.09LNGIVNFYY218 pKa = 10.77NGVIFSS224 pKa = 3.89

Molecular weight: 25.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1113

131

262

185.5

21.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.953 ± 0.872

1.617 ± 0.472

6.11 ± 1.121

5.75 ± 0.988

4.403 ± 0.747

5.211 ± 1.168

2.695 ± 0.631

5.481 ± 0.523

5.301 ± 0.914

7.907 ± 0.922

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.688

4.672 ± 0.877

4.942 ± 0.417

3.684 ± 0.809

7.817 ± 1.805

9.344 ± 2.013

6.739 ± 1.636

6.289 ± 1.247

1.527 ± 0.431

4.043 ± 0.633
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski