Jiella endophytica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Aurantimonadaceae; Jiella

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4547 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8R9K4|A0A4Y8R9K4_9RHIZ ABC transporter substrate-binding protein OS=Jiella endophytica OX=2558362 GN=E3C22_23485 PE=3 SV=1
MM1 pKa = 7.09IRR3 pKa = 11.84SRR5 pKa = 11.84FASLLFVGTSLAGMPALAQEE25 pKa = 4.98IPTSPPADD33 pKa = 3.75FSNGRR38 pKa = 11.84TPDD41 pKa = 3.89DD42 pKa = 4.62PSDD45 pKa = 3.65LPYY48 pKa = 11.5LMDD51 pKa = 4.45SYY53 pKa = 12.05VEE55 pKa = 4.56LLLDD59 pKa = 4.75DD60 pKa = 5.43PDD62 pKa = 5.3VMTQNYY68 pKa = 9.73DD69 pKa = 3.65YY70 pKa = 11.57VVTATQNRR78 pKa = 11.84TDD80 pKa = 3.91EE81 pKa = 4.12QTLAAIHH88 pKa = 7.48DD89 pKa = 4.2DD90 pKa = 3.6RR91 pKa = 11.84TNQQYY96 pKa = 11.42SVMNGLGVLTSIVMTGTGASADD118 pKa = 3.69GTTPASLTPTTYY130 pKa = 11.5ADD132 pKa = 3.37ATIADD137 pKa = 4.07YY138 pKa = 10.91EE139 pKa = 4.21ADD141 pKa = 3.27INYY144 pKa = 10.09LNGASWGTTTFADD157 pKa = 4.35GTATPLASAVTFIQDD172 pKa = 3.1IVRR175 pKa = 11.84ADD177 pKa = 3.49ASTEE181 pKa = 3.7PSKK184 pKa = 10.67RR185 pKa = 11.84TFDD188 pKa = 3.97RR189 pKa = 11.84LMEE192 pKa = 4.68NNDD195 pKa = 3.31TYY197 pKa = 11.08RR198 pKa = 11.84QYY200 pKa = 11.29EE201 pKa = 4.34SQYY204 pKa = 10.29QDD206 pKa = 3.14YY207 pKa = 10.93NVISNPDD214 pKa = 3.37ALTTEE219 pKa = 4.23DD220 pKa = 3.76TADD223 pKa = 4.56FIIPAYY229 pKa = 10.08LSNYY233 pKa = 9.17DD234 pKa = 3.35VPEE237 pKa = 4.25EE238 pKa = 4.1YY239 pKa = 9.64GTVEE243 pKa = 3.24NWVRR247 pKa = 11.84GFTVTQEE254 pKa = 4.3MIDD257 pKa = 3.77EE258 pKa = 4.64NGGEE262 pKa = 4.43PITVEE267 pKa = 5.11GLGLWTDD274 pKa = 4.04GVFTPATFGVGDD286 pKa = 4.01YY287 pKa = 11.06VPGIGTSPRR296 pKa = 11.84PYY298 pKa = 9.59RR299 pKa = 11.84VSDD302 pKa = 3.55NVAVPEE308 pKa = 4.52LLEE311 pKa = 4.14QVANTTNPYY320 pKa = 10.72ADD322 pKa = 3.14GSMPSGHH329 pKa = 6.49TSSAWTEE336 pKa = 3.85ALGLAFLVPQEE347 pKa = 4.11MQSLVTRR354 pKa = 11.84ASEE357 pKa = 4.03LGEE360 pKa = 3.94DD361 pKa = 3.7RR362 pKa = 11.84ILAGEE367 pKa = 4.44HH368 pKa = 6.37SPLDD372 pKa = 3.88VIGGRR377 pKa = 11.84IIAEE381 pKa = 4.71AIAATNIYY389 pKa = 9.77DD390 pKa = 4.54ALYY393 pKa = 10.44DD394 pKa = 4.09DD395 pKa = 4.78EE396 pKa = 5.71GNRR399 pKa = 11.84LDD401 pKa = 3.66WTDD404 pKa = 3.42PANEE408 pKa = 3.91EE409 pKa = 4.08AYY411 pKa = 10.16AVYY414 pKa = 8.61TAYY417 pKa = 9.78TEE419 pKa = 4.16TQAYY423 pKa = 8.44IAEE426 pKa = 4.5ACGTATVDD434 pKa = 3.11EE435 pKa = 4.79CVALGGDD442 pKa = 4.14GTSVAYY448 pKa = 8.32GTAEE452 pKa = 3.82ANKK455 pKa = 10.21AAFLYY460 pKa = 10.45RR461 pKa = 11.84MTYY464 pKa = 10.04GFEE467 pKa = 4.25ATSEE471 pKa = 4.37PYY473 pKa = 10.69RR474 pKa = 11.84MAVSDD479 pKa = 4.33VPVQAQVLLLTRR491 pKa = 11.84FPYY494 pKa = 9.62LTDD497 pKa = 3.46EE498 pKa = 4.15QRR500 pKa = 11.84LEE502 pKa = 4.22VLASTALDD510 pKa = 3.29ADD512 pKa = 4.01YY513 pKa = 10.96PLISGNTYY521 pKa = 10.79DD522 pKa = 4.86GWGKK526 pKa = 10.82LNLYY530 pKa = 9.83DD531 pKa = 4.27AADD534 pKa = 4.35GYY536 pKa = 11.72GYY538 pKa = 10.58FEE540 pKa = 5.48NDD542 pKa = 2.94VTVTMDD548 pKa = 3.8ASLGGYY554 pKa = 8.63NAADD558 pKa = 3.17TWANDD563 pKa = 2.99IAGPGGLVKK572 pKa = 10.81DD573 pKa = 4.04GTGKK577 pKa = 8.71LTLSGQDD584 pKa = 3.18TYY586 pKa = 11.64TGDD589 pKa = 3.44TVVAGGTLAIDD600 pKa = 4.06GSIVSNVRR608 pKa = 11.84VQRR611 pKa = 11.84RR612 pKa = 11.84AVLRR616 pKa = 11.84GVGVIGGGVTVEE628 pKa = 4.82DD629 pKa = 4.84GGTLAPGNSPGTLTVANSVTLSDD652 pKa = 3.52GAMSEE657 pKa = 4.01FDD659 pKa = 3.15IDD661 pKa = 3.84GTGTEE666 pKa = 4.47IGAGNYY672 pKa = 9.5SRR674 pKa = 11.84LEE676 pKa = 3.91LVGEE680 pKa = 4.22DD681 pKa = 4.81SIFTADD687 pKa = 4.05GEE689 pKa = 4.8LIPLLRR695 pKa = 11.84GITGDD700 pKa = 3.22ATNSYY705 pKa = 9.02TPDD708 pKa = 3.08IGTQFQIVVAEE719 pKa = 4.2GGVAGSYY726 pKa = 11.09DD727 pKa = 4.51SLTQPDD733 pKa = 4.02GLADD737 pKa = 3.47GTRR740 pKa = 11.84FDD742 pKa = 4.75TIYY745 pKa = 10.91GSNDD749 pKa = 2.55LRR751 pKa = 11.84LVVTPTAYY759 pKa = 10.09EE760 pKa = 4.34DD761 pKa = 3.81YY762 pKa = 10.6AASGNAANVAEE773 pKa = 4.59ALDD776 pKa = 4.19AGRR779 pKa = 11.84QAAGIRR785 pKa = 11.84LDD787 pKa = 4.76DD788 pKa = 4.95DD789 pKa = 3.98AASLYY794 pKa = 10.49DD795 pKa = 3.81ALYY798 pKa = 10.7ALSAADD804 pKa = 4.63LPAALGDD811 pKa = 4.47LAPQAYY817 pKa = 9.94GDD819 pKa = 3.87ALLVNRR825 pKa = 11.84DD826 pKa = 2.96SWYY829 pKa = 10.74LGSDD833 pKa = 3.07AVNRR837 pKa = 11.84AIEE840 pKa = 3.89ARR842 pKa = 11.84RR843 pKa = 11.84GGVVDD848 pKa = 3.78PTMQSAAQHH857 pKa = 6.2GMTAWLTALGQKK869 pKa = 10.52ASIDD873 pKa = 3.64SDD875 pKa = 3.82PASGYY880 pKa = 10.65DD881 pKa = 3.23QTLGGLIVGLDD892 pKa = 3.48GEE894 pKa = 4.59MGDD897 pKa = 4.34GFLLGASLGYY907 pKa = 10.98VNLDD911 pKa = 3.03ADD913 pKa = 4.87LDD915 pKa = 3.93QGASFSGDD923 pKa = 3.11SLQGRR928 pKa = 11.84IYY930 pKa = 10.13GSYY933 pKa = 9.59RR934 pKa = 11.84RR935 pKa = 11.84GIAFVDD941 pKa = 4.26GQFGFAYY948 pKa = 9.66TDD950 pKa = 3.8GEE952 pKa = 4.17VDD954 pKa = 3.05RR955 pKa = 11.84GVAGFGGMHH964 pKa = 7.11GDD966 pKa = 3.72ADD968 pKa = 3.89STSFGGEE975 pKa = 3.13IRR977 pKa = 11.84TGLRR981 pKa = 11.84YY982 pKa = 9.97GDD984 pKa = 3.93EE985 pKa = 4.2ATSFEE990 pKa = 4.33PSIALRR996 pKa = 11.84GVSVQLDD1003 pKa = 3.88GATEE1007 pKa = 4.05SGPAAVSFDD1016 pKa = 3.72DD1017 pKa = 4.26QSFDD1021 pKa = 3.5SLQSVLALGVSHH1033 pKa = 7.57RR1034 pKa = 11.84IALADD1039 pKa = 3.79GMSLTPAARR1048 pKa = 11.84IGWSHH1053 pKa = 6.39EE1054 pKa = 3.9FSDD1057 pKa = 3.6TDD1059 pKa = 3.88GAVTAAFVGAPGASFEE1075 pKa = 4.61TVGASIGRR1083 pKa = 11.84DD1084 pKa = 3.08AALVGVSAEE1093 pKa = 4.16FANGGAGSVFASYY1106 pKa = 10.62EE1107 pKa = 3.83GAFAEE1112 pKa = 4.45NQSSHH1117 pKa = 5.85SVTAGLKK1124 pKa = 10.01FSWW1127 pKa = 3.68

Molecular weight: 118.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8RPI7|A0A4Y8RPI7_9RHIZ ABC transporter substrate-binding protein OS=Jiella endophytica OX=2558362 GN=E3C22_09120 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38GGRR28 pKa = 11.84KK29 pKa = 9.14VIAARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.96RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4547

0

4547

1455190

25

3280

320.0

34.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.024 ± 0.055

0.799 ± 0.01

5.88 ± 0.039

6.132 ± 0.037

3.828 ± 0.026

8.933 ± 0.04

1.861 ± 0.016

5.123 ± 0.03

3.18 ± 0.03

9.845 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.416 ± 0.018

2.329 ± 0.021

5.191 ± 0.033

2.734 ± 0.021

7.229 ± 0.04

5.55 ± 0.024

5.198 ± 0.029

7.429 ± 0.032

1.183 ± 0.013

2.134 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski