Rhodococcus sp. PBTS 1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3895 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143Q9Q7|A0A143Q9Q7_9NOCA Uncharacterized protein OS=Rhodococcus sp. PBTS 1 OX=1653478 GN=A3Q40_02042 PE=4 SV=1
MM1 pKa = 7.64VDD3 pKa = 4.3VRR5 pKa = 11.84DD6 pKa = 3.85QLPPGLPPDD15 pKa = 4.62PFAGDD20 pKa = 3.58PADD23 pKa = 4.15PSAALDD29 pKa = 4.6AIEE32 pKa = 5.17PGQPLDD38 pKa = 3.61PQEE41 pKa = 4.14RR42 pKa = 11.84LAVEE46 pKa = 4.17EE47 pKa = 4.75DD48 pKa = 3.54LADD51 pKa = 3.98LAVYY55 pKa = 8.37EE56 pKa = 4.54ALLAHH61 pKa = 6.58RR62 pKa = 11.84GVRR65 pKa = 11.84GLVVCCEE72 pKa = 4.65DD73 pKa = 3.49CQQDD77 pKa = 4.19HH78 pKa = 5.63FHH80 pKa = 7.03DD81 pKa = 3.84WDD83 pKa = 3.57MLRR86 pKa = 11.84ANLLQLLVDD95 pKa = 4.02GTVRR99 pKa = 11.84PHH101 pKa = 6.34EE102 pKa = 4.35PAYY105 pKa = 10.69DD106 pKa = 5.07PIPDD110 pKa = 4.61AYY112 pKa = 9.72VTWDD116 pKa = 3.35YY117 pKa = 11.62CRR119 pKa = 11.84GYY121 pKa = 11.43ADD123 pKa = 5.07ASMNDD128 pKa = 3.49ALHH131 pKa = 7.07GDD133 pKa = 3.84GFDD136 pKa = 3.27AA137 pKa = 6.02

Molecular weight: 15.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143QE49|A0A143QE49_9NOCA Uncharacterized protein OS=Rhodococcus sp. PBTS 1 OX=1653478 GN=A3Q40_03813 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84AKK40 pKa = 10.37GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3895

0

3895

1294763

29

11390

332.4

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.488 ± 0.047

0.668 ± 0.012

6.938 ± 0.037

5.137 ± 0.036

2.843 ± 0.023

9.0 ± 0.041

2.066 ± 0.018

3.57 ± 0.026

1.71 ± 0.031

9.726 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.745 ± 0.016

1.714 ± 0.02

5.704 ± 0.034

2.32 ± 0.022

7.857 ± 0.043

5.631 ± 0.025

6.66 ± 0.03

9.916 ± 0.047

1.407 ± 0.016

1.899 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski