Rhinopithecus bieti (Black snub-nosed monkey) (Pygathrix bieti)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates;

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42660 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2K6MDG9|A0A2K6MDG9_RHIBE E3 ubiquitin-protein ligase PPP1R11 OS=Rhinopithecus bieti OX=61621 PE=4 SV=1
MM1 pKa = 7.5SEE3 pKa = 4.04KK4 pKa = 10.31KK5 pKa = 10.32QPVDD9 pKa = 3.98LGLLEE14 pKa = 4.72EE15 pKa = 5.08DD16 pKa = 4.95DD17 pKa = 4.2EE18 pKa = 5.16FEE20 pKa = 4.5EE21 pKa = 6.21FPAEE25 pKa = 3.92DD26 pKa = 3.4WAGLDD31 pKa = 3.67EE32 pKa = 6.8DD33 pKa = 4.65EE34 pKa = 5.59DD35 pKa = 3.98AHH37 pKa = 7.46VWEE40 pKa = 5.76DD41 pKa = 3.11NWDD44 pKa = 3.91DD45 pKa = 5.56DD46 pKa = 4.79NVEE49 pKa = 4.84DD50 pKa = 5.62DD51 pKa = 4.76FSNQLRR57 pKa = 11.84ATVLLMIKK65 pKa = 10.19VYY67 pKa = 7.17EE68 pKa = 4.29TPYY71 pKa = 10.43GCYY74 pKa = 9.49ILHH77 pKa = 5.98QKK79 pKa = 10.37GRR81 pKa = 11.84MCIAFLCCC89 pKa = 5.03

Molecular weight:
10.44 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2K6L5D1|A0A2K6L5D1_RHIBE Uncharacterized protein OS=Rhinopithecus bieti OX=61621 GN=HSD11B2 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight:
3.46 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20855

21805

42660

22132529

12

8476

518.8

57.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.893 ± 0.013

2.215 ± 0.011

4.861 ± 0.008

7.159 ± 0.015

3.632 ± 0.009

6.459 ± 0.016

2.612 ± 0.006

4.418 ± 0.009

5.843 ± 0.017

9.883 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.005

3.679 ± 0.008

6.166 ± 0.017

4.802 ± 0.011

5.66 ± 0.012

8.319 ± 0.016

5.32 ± 0.008

6.012 ± 0.01

1.214 ± 0.004

2.665 ± 0.007

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski