Bordetella phage CN2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vojvodinavirus; Bordetella virus CN2

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0W9F7|A0A2D0W9F7_9CAUD Virion structural protein OS=Bordetella phage CN2 OX=1916124 PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 6.08PEE4 pKa = 4.47NLPPPIATLGPDD16 pKa = 3.0VTFYY20 pKa = 11.54DD21 pKa = 4.63DD22 pKa = 4.11GDD24 pKa = 3.83VTEE27 pKa = 4.81GLTYY31 pKa = 9.98YY32 pKa = 10.72YY33 pKa = 10.51RR34 pKa = 11.84VGAFNAARR42 pKa = 11.84EE43 pKa = 4.36VVSAEE48 pKa = 4.13LEE50 pKa = 4.21APADD54 pKa = 3.99PNVSQARR61 pKa = 11.84IIWRR65 pKa = 11.84ILIQDD70 pKa = 3.53TRR72 pKa = 11.84SGDD75 pKa = 4.01EE76 pKa = 3.92YY77 pKa = 11.19ACWVSEE83 pKa = 3.44IKK85 pKa = 9.99MRR87 pKa = 11.84VLGGPNLAVDD97 pKa = 4.74GLASASSFYY106 pKa = 10.2STPDD110 pKa = 2.63DD111 pKa = 3.84WGPGRR116 pKa = 11.84AFDD119 pKa = 4.73GSPATGWSSNSGAPLPQWIAYY140 pKa = 7.34EE141 pKa = 4.13LTSPAALTEE150 pKa = 3.9ISIQAGNNAGRR161 pKa = 11.84AARR164 pKa = 11.84APRR167 pKa = 11.84TFQIQSSMDD176 pKa = 3.11GTIWEE181 pKa = 4.51TRR183 pKa = 11.84FTATDD188 pKa = 3.63EE189 pKa = 4.42PAWDD193 pKa = 3.94PGEE196 pKa = 3.78IRR198 pKa = 11.84TYY200 pKa = 10.35TFF202 pKa = 3.32

Molecular weight: 21.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0W988|A0A2D0W988_9CAUD aGPT-Pplase2 domain-containing protein OS=Bordetella phage CN2 OX=1916124 PE=4 SV=1
MM1 pKa = 7.36SWWAWINIVAAVAGFLYY18 pKa = 10.04WLRR21 pKa = 11.84GANQGFCLHH30 pKa = 6.29RR31 pKa = 11.84WEE33 pKa = 4.69AGRR36 pKa = 11.84NRR38 pKa = 11.84FLTGALTCSKK48 pKa = 9.71CGRR51 pKa = 11.84IMYY54 pKa = 8.38PEE56 pKa = 4.19RR57 pKa = 11.84MRR59 pKa = 11.84FLRR62 pKa = 11.84LYY64 pKa = 9.68RR65 pKa = 11.84ALGRR69 pKa = 11.84IEE71 pKa = 4.34MEE73 pKa = 4.19NKK75 pKa = 9.06QQ76 pKa = 3.4

Molecular weight: 9.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

19328

38

939

238.6

26.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.756 ± 0.379

0.978 ± 0.087

6.1 ± 0.192

7.305 ± 0.242

3.658 ± 0.148

7.952 ± 0.235

1.764 ± 0.147

4.418 ± 0.15

4.258 ± 0.268

8.739 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.049 ± 0.116

3.554 ± 0.171

5.298 ± 0.249

3.999 ± 0.124

7.124 ± 0.256

4.682 ± 0.208

5.427 ± 0.258

7.057 ± 0.195

1.976 ± 0.137

2.794 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski