Lactococcus phage CHPC52

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus CHPC52

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A650ESM6|A0A650ESM6_9CAUD Uncharacterized protein OS=Lactococcus phage CHPC52 OX=2675251 GN=CHPC52_000697 PE=4 SV=1
MM1 pKa = 7.56EE2 pKa = 5.5FDD4 pKa = 6.13SYY6 pKa = 11.42IDD8 pKa = 3.32WYY10 pKa = 11.58NNLLTMPLNDD20 pKa = 3.86VILGVKK26 pKa = 8.96DD27 pKa = 3.57TIEE30 pKa = 4.55DD31 pKa = 3.52KK32 pKa = 10.66TVYY35 pKa = 10.61LSLSNSKK42 pKa = 9.76VIKK45 pKa = 9.58MDD47 pKa = 3.03NTSFVMGYY55 pKa = 9.13YY56 pKa = 8.74YY57 pKa = 10.45QVVLSVKK64 pKa = 10.52DD65 pKa = 3.51VDD67 pKa = 4.41DD68 pKa = 4.44EE69 pKa = 4.64LVGLVGNVLQNGWNMTNWSEE89 pKa = 4.32NSHH92 pKa = 6.58LYY94 pKa = 10.7NYY96 pKa = 8.61TGTVYY101 pKa = 10.42LPCGAGGQAWQQ112 pKa = 3.59

Molecular weight: 12.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A650ET14|A0A650ET14_9CAUD Single stranded DNA-binding protein OS=Lactococcus phage CHPC52 OX=2675251 GN=CHPC52_000703 PE=4 SV=1
MM1 pKa = 7.46CKK3 pKa = 9.75KK4 pKa = 10.38RR5 pKa = 11.84KK6 pKa = 5.78YY7 pKa = 8.56TKK9 pKa = 9.78MGALYY14 pKa = 10.54SIANAQHH21 pKa = 5.77NKK23 pKa = 9.73KK24 pKa = 10.11SKK26 pKa = 10.27KK27 pKa = 10.04NKK29 pKa = 8.95ADD31 pKa = 4.29KK32 pKa = 10.69IPVRR36 pKa = 11.84AYY38 pKa = 9.48HH39 pKa = 6.61CKK41 pKa = 8.93WCNLYY46 pKa = 10.43HH47 pKa = 7.32LSSQQRR53 pKa = 11.84LNIKK57 pKa = 9.21TGVIGG62 pKa = 3.97

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

8651

29

916

160.2

18.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.115 ± 0.578

0.659 ± 0.162

5.537 ± 0.29

7.19 ± 0.55

4.473 ± 0.319

6.346 ± 0.678

1.179 ± 0.196

6.959 ± 0.293

9.109 ± 0.443

8.843 ± 0.462

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.508 ± 0.154

6.704 ± 0.265

2.173 ± 0.257

3.607 ± 0.194

3.306 ± 0.287

6.311 ± 0.39

6.728 ± 0.322

6.589 ± 0.383

1.41 ± 0.143

4.254 ± 0.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski