Mycobacterium virus Ramsey

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5U5L9|B5U5L9_9CAUD Uncharacterized protein OS=Mycobacterium virus Ramsey OX=563123 GN=72 PE=4 SV=1
MM1 pKa = 6.72NTSTNRR7 pKa = 11.84EE8 pKa = 4.05YY9 pKa = 10.46PSYY12 pKa = 11.1AGDD15 pKa = 3.75LALTLDD21 pKa = 4.21TSGSTAKK28 pKa = 9.04ITVEE32 pKa = 4.07EE33 pKa = 4.15NGEE36 pKa = 4.43TVTVDD41 pKa = 4.29LDD43 pKa = 3.65AEE45 pKa = 4.41TARR48 pKa = 11.84DD49 pKa = 3.25LGTRR53 pKa = 11.84VIAYY57 pKa = 9.16NGSPSTWTIEE67 pKa = 3.81PMHH70 pKa = 6.62FGDD73 pKa = 4.99VEE75 pKa = 4.34LTVEE79 pKa = 4.79DD80 pKa = 4.37GQILLALPEE89 pKa = 4.41ATPFDD94 pKa = 4.63LGEE97 pKa = 4.24GDD99 pKa = 3.68IDD101 pKa = 4.3PFVHH105 pKa = 7.71DD106 pKa = 4.54FGQRR110 pKa = 11.84LIVWAGVNLEE120 pKa = 4.13VSADD124 pKa = 3.38AA125 pKa = 5.44

Molecular weight: 13.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5U5J7|B5U5J7_9CAUD Uncharacterized protein OS=Mycobacterium virus Ramsey OX=563123 GN=50 PE=4 SV=1
MM1 pKa = 7.51SSATSRR7 pKa = 11.84IDD9 pKa = 3.01KK10 pKa = 8.81TGPGARR16 pKa = 11.84QRR18 pKa = 11.84IPTPNPITGLSRR30 pKa = 11.84EE31 pKa = 4.16GLAVDD36 pKa = 3.87ATHH39 pKa = 6.59EE40 pKa = 4.04EE41 pKa = 3.91WRR43 pKa = 11.84PVVGFEE49 pKa = 4.46GLYY52 pKa = 10.11EE53 pKa = 3.99VSNLGRR59 pKa = 11.84VRR61 pKa = 11.84SLDD64 pKa = 2.98HH65 pKa = 6.46FARR68 pKa = 11.84GRR70 pKa = 11.84SGSKK74 pKa = 9.35RR75 pKa = 11.84LIRR78 pKa = 11.84GRR80 pKa = 11.84VLRR83 pKa = 11.84PAPRR87 pKa = 11.84TSGHH91 pKa = 5.84LTVALGRR98 pKa = 11.84NGGSKK103 pKa = 10.31DD104 pKa = 3.35VHH106 pKa = 5.79TLVATAFIGPRR117 pKa = 11.84PEE119 pKa = 4.96GMEE122 pKa = 4.52CCHH125 pKa = 6.98QDD127 pKa = 3.81GDD129 pKa = 4.02PTNNRR134 pKa = 11.84VEE136 pKa = 4.07NLRR139 pKa = 11.84WDD141 pKa = 3.57TRR143 pKa = 11.84SANRR147 pKa = 11.84LDD149 pKa = 3.89SVRR152 pKa = 11.84HH153 pKa = 4.99GTHH156 pKa = 4.89QAVKK160 pKa = 9.03KK161 pKa = 6.01THH163 pKa = 6.55CKK165 pKa = 9.72HH166 pKa = 5.21GHH168 pKa = 5.66EE169 pKa = 4.33FTPEE173 pKa = 3.46NTLVQRR179 pKa = 11.84GKK181 pKa = 9.87HH182 pKa = 4.27RR183 pKa = 11.84RR184 pKa = 11.84CRR186 pKa = 11.84EE187 pKa = 3.7CHH189 pKa = 6.58RR190 pKa = 11.84LDD192 pKa = 3.54SQKK195 pKa = 11.0GYY197 pKa = 9.93PRR199 pKa = 4.67

Molecular weight: 22.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

18792

27

1232

174.0

19.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.627 ± 0.405

1.181 ± 0.171

6.572 ± 0.243

5.832 ± 0.28

2.916 ± 0.203

8.961 ± 0.605

2.15 ± 0.209

4.465 ± 0.154

3.374 ± 0.165

7.322 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.114

3.496 ± 0.161

5.992 ± 0.198

3.608 ± 0.222

6.806 ± 0.417

5.928 ± 0.302

6.503 ± 0.261

7.269 ± 0.252

2.23 ± 0.163

2.512 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski