Ruminococcus sp. CAG:254

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1961 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5UYD1|R5UYD1_9FIRM Stage 0 sporulation protein A homolog OS=Ruminococcus sp. CAG:254 OX=1262953 GN=BN566_01114 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 4.03VNGEE6 pKa = 4.2YY7 pKa = 9.97PDD9 pKa = 3.53EE10 pKa = 4.62TIEE13 pKa = 4.22KK14 pKa = 9.02VVEE17 pKa = 3.82KK18 pKa = 10.4SVYY21 pKa = 7.03TRR23 pKa = 11.84RR24 pKa = 11.84IGDD27 pKa = 3.79ILPDD31 pKa = 3.74NLVDD35 pKa = 6.01LDD37 pKa = 4.41DD38 pKa = 4.59AVNLLGIVSFLDD50 pKa = 3.11SCTAIGAAYY59 pKa = 8.23TAFLNDD65 pKa = 4.67AMTADD70 pKa = 3.84YY71 pKa = 10.75SYY73 pKa = 9.98TLQDD77 pKa = 3.57GTTGTYY83 pKa = 9.65RR84 pKa = 11.84EE85 pKa = 4.05RR86 pKa = 11.84FKK88 pKa = 11.46DD89 pKa = 3.36SLYY92 pKa = 11.06ANSVPFAEE100 pKa = 5.9IADD103 pKa = 3.96CDD105 pKa = 3.57QNGRR109 pKa = 11.84IEE111 pKa = 5.31KK112 pKa = 10.35NDD114 pKa = 3.36ADD116 pKa = 4.84LLVTYY121 pKa = 8.4YY122 pKa = 10.78AQAAAGQSPEE132 pKa = 4.07SLINNEE138 pKa = 3.82LSKK141 pKa = 11.06VVYY144 pKa = 10.76ADD146 pKa = 2.8ITMM149 pKa = 4.45

Molecular weight: 16.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5UY21|R5UY21_9FIRM Uncharacterized protein OS=Ruminococcus sp. CAG:254 OX=1262953 GN=BN566_00760 PE=4 SV=1
MM1 pKa = 7.17EE2 pKa = 5.39RR3 pKa = 11.84APRR6 pKa = 11.84MGGKK10 pKa = 9.71RR11 pKa = 11.84PRR13 pKa = 11.84KK14 pKa = 7.89KK15 pKa = 10.22VCMFCVDD22 pKa = 3.53RR23 pKa = 11.84VDD25 pKa = 3.95VIDD28 pKa = 4.14YY29 pKa = 11.09KK30 pKa = 11.29DD31 pKa = 3.03IAKK34 pKa = 9.94LRR36 pKa = 11.84KK37 pKa = 9.83CMTEE41 pKa = 3.53RR42 pKa = 11.84AKK44 pKa = 10.45ILPRR48 pKa = 11.84RR49 pKa = 11.84VTGTCAYY56 pKa = 9.21HH57 pKa = 5.53QRR59 pKa = 11.84KK60 pKa = 7.65LTTAIKK66 pKa = 9.99RR67 pKa = 11.84ARR69 pKa = 11.84HH70 pKa = 4.82VALLPYY76 pKa = 10.6VSDD79 pKa = 3.6

Molecular weight: 9.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1961

0

1961

616928

31

3562

314.6

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.596 ± 0.06

1.871 ± 0.031

5.506 ± 0.058

6.676 ± 0.054

3.966 ± 0.044

6.738 ± 0.061

1.986 ± 0.027

6.478 ± 0.047

5.624 ± 0.045

9.203 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.844 ± 0.03

4.01 ± 0.058

3.851 ± 0.042

4.531 ± 0.05

4.135 ± 0.047

5.791 ± 0.047

6.621 ± 0.081

6.707 ± 0.041

0.971 ± 0.023

3.891 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski