Kocuria flava

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Kocuria

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3024 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U3GQ82|A0A0U3GQ82_9MICC Alpha/beta hydrolase OS=Kocuria flava OX=446860 GN=AS188_15755 PE=4 SV=1
MM1 pKa = 7.41AASTVPLLVLTGCASDD17 pKa = 4.24AGAEE21 pKa = 3.92AAATGVVDD29 pKa = 3.1YY30 pKa = 10.59WMWDD34 pKa = 3.58TNQLPAYY41 pKa = 7.67QKK43 pKa = 9.43CAEE46 pKa = 4.27MFEE49 pKa = 5.04AEE51 pKa = 5.52HH52 pKa = 7.29PDD54 pKa = 2.91QDD56 pKa = 4.58VRR58 pKa = 11.84ITQIGWGDD66 pKa = 3.62YY67 pKa = 6.46WTKK70 pKa = 9.96LTAGFIAGTGPDD82 pKa = 3.23VFTNHH87 pKa = 5.29VSKK90 pKa = 11.05YY91 pKa = 8.13PQFAEE96 pKa = 4.34LEE98 pKa = 4.25VLAPLEE104 pKa = 4.14EE105 pKa = 4.82HH106 pKa = 7.02PATAALEE113 pKa = 4.24PADD116 pKa = 4.04YY117 pKa = 10.88QDD119 pKa = 4.22GLVDD123 pKa = 4.49LWTGPDD129 pKa = 3.02GHH131 pKa = 6.62QYY133 pKa = 10.72GVPKK137 pKa = 10.6DD138 pKa = 3.36WDD140 pKa = 4.05TVAVLYY146 pKa = 10.92NEE148 pKa = 4.23EE149 pKa = 4.01LVAEE153 pKa = 4.85AGLSPEE159 pKa = 4.99DD160 pKa = 3.77LEE162 pKa = 4.42GWRR165 pKa = 11.84WNPEE169 pKa = 3.9DD170 pKa = 3.93GGSFEE175 pKa = 5.97DD176 pKa = 5.0LVAHH180 pKa = 6.34LTIDD184 pKa = 3.69EE185 pKa = 4.33NGVRR189 pKa = 11.84GDD191 pKa = 3.62EE192 pKa = 4.06PGFDD196 pKa = 3.72KK197 pKa = 11.38DD198 pKa = 3.42HH199 pKa = 6.0VAVYY203 pKa = 10.8GLGISEE209 pKa = 5.02AGGSTAGQGQWSAFAASNGWRR230 pKa = 11.84VTDD233 pKa = 3.6EE234 pKa = 4.45PLWGDD239 pKa = 4.26HH240 pKa = 6.3YY241 pKa = 11.76NVDD244 pKa = 4.53DD245 pKa = 4.52PALHH249 pKa = 6.4EE250 pKa = 4.16TLDD253 pKa = 3.61WYY255 pKa = 11.1FGLTDD260 pKa = 3.62KK261 pKa = 11.37GYY263 pKa = 9.0MPAYY267 pKa = 9.47GQFNAAEE274 pKa = 4.31GVNAQLASGSAAMVLDD290 pKa = 4.43GAWMISTYY298 pKa = 11.38AAMDD302 pKa = 5.1DD303 pKa = 3.71ISVGTARR310 pKa = 11.84TPVGPDD316 pKa = 3.18GTSTSMMNGLADD328 pKa = 4.88SIVKK332 pKa = 8.91DD333 pKa = 3.46TDD335 pKa = 3.52NPEE338 pKa = 4.58GAAQWAAFLGSAQCQQEE355 pKa = 4.27VARR358 pKa = 11.84AGVVFPARR366 pKa = 11.84QDD368 pKa = 3.43ATPIAADD375 pKa = 4.58AYY377 pKa = 9.16RR378 pKa = 11.84QMGIDD383 pKa = 4.12PEE385 pKa = 4.27PFIAPVEE392 pKa = 4.26EE393 pKa = 4.74GSTVYY398 pKa = 10.49FPVTKK403 pKa = 10.67FGADD407 pKa = 3.19VSALMTPALEE417 pKa = 5.13DD418 pKa = 3.0IYY420 pKa = 11.36ANRR423 pKa = 11.84VPASTLTGINDD434 pKa = 5.16AINVLFEE441 pKa = 4.03TDD443 pKa = 3.28EE444 pKa = 4.64TEE446 pKa = 4.23GSS448 pKa = 3.46

Molecular weight: 47.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U3HVS4|A0A0U3HVS4_9MICC Uncharacterized protein OS=Kocuria flava OX=446860 GN=AS188_03815 PE=4 SV=1
MM1 pKa = 7.08VPPWSSTIVRR11 pKa = 11.84ATARR15 pKa = 11.84PSPRR19 pKa = 11.84PGIALIRR26 pKa = 11.84ALRR29 pKa = 11.84LRR31 pKa = 11.84PKK33 pKa = 10.43RR34 pKa = 11.84SRR36 pKa = 11.84TRR38 pKa = 11.84SASSAGRR45 pKa = 11.84PIPRR49 pKa = 11.84SATRR53 pKa = 11.84SRR55 pKa = 11.84AVSPSRR61 pKa = 11.84STRR64 pKa = 11.84TVTGPPSGEE73 pKa = 3.79

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3024

0

3024

990024

37

1667

327.4

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.19 ± 0.079

0.64 ± 0.012

5.698 ± 0.038

6.304 ± 0.047

2.722 ± 0.025

9.564 ± 0.046

2.355 ± 0.023

2.975 ± 0.037

1.616 ± 0.027

10.536 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.706 ± 0.017

1.612 ± 0.023

6.211 ± 0.044

2.753 ± 0.024

8.176 ± 0.058

4.515 ± 0.03

5.807 ± 0.032

9.262 ± 0.043

1.504 ± 0.019

1.854 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski