Lactococcus fujiensis JCM 16395

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Lactococcus; Lactococcus fujiensis

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2024 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A5RPV7|A0A2A5RPV7_9LACT Uncharacterized protein OS=Lactococcus fujiensis JCM 16395 OX=1291764 GN=RT41_GL000228 PE=4 SV=1
MM1 pKa = 7.75KK2 pKa = 9.12KK3 pKa = 8.83TSLLITSLALLFGLIPIVANADD25 pKa = 3.92TVSGTTGSGTATITLTPGTLQFISVPDD52 pKa = 3.71FTFGSQAVGVTSVTSTALATGALTVGDD79 pKa = 4.04YY80 pKa = 11.25SGGITGYY87 pKa = 10.83NVTAQMTQGFTNGTSTLVTPNFTVSLGNSEE117 pKa = 5.33DD118 pKa = 3.8GALTGISGNIATTSTKK134 pKa = 9.96IASGAANTSAGQEE147 pKa = 3.84ATIGGDD153 pKa = 3.33AQLTITNASGTMYY166 pKa = 9.89TQGNYY171 pKa = 10.09SATIDD176 pKa = 3.57YY177 pKa = 9.06TLGVGII183 pKa = 5.11

Molecular weight: 18.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A5RLL6|A0A2A5RLL6_9LACT Heme/copper-type cytochrome/quinol oxidase subunit 1 OS=Lactococcus fujiensis JCM 16395 OX=1291764 GN=RT41_GL001471 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 5.97KK9 pKa = 10.04KK10 pKa = 9.24PRR12 pKa = 11.84KK13 pKa = 4.63TTHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.97NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.65GRR39 pKa = 11.84ASLTVV44 pKa = 3.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2024

0

2024

594089

37

3568

293.5

32.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.431 ± 0.06

0.454 ± 0.013

5.43 ± 0.054

6.645 ± 0.067

4.867 ± 0.047

6.607 ± 0.051

1.866 ± 0.027

7.813 ± 0.058

6.98 ± 0.055

9.653 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.03

5.242 ± 0.05

3.264 ± 0.028

3.786 ± 0.036

3.691 ± 0.044

6.474 ± 0.059

5.947 ± 0.07

6.661 ± 0.048

0.981 ± 0.021

3.546 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski