Vibrio phage Saratov-12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; unclassified Zobellviridae

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XV87|A0A6G6XV87_9CAUD Uncharacterized protein OS=Vibrio phage Saratov-12 OX=2712943 GN=Saratov12_00067 PE=4 SV=1
MM1 pKa = 7.48AKK3 pKa = 9.63IVLSDD8 pKa = 3.52VTSGSSVTINRR19 pKa = 11.84NFQKK23 pKa = 10.4IEE25 pKa = 3.87DD26 pKa = 4.05ALNDD30 pKa = 3.53EE31 pKa = 4.7VLFRR35 pKa = 11.84DD36 pKa = 3.91NPEE39 pKa = 4.08GEE41 pKa = 4.29PNQMEE46 pKa = 4.67SDD48 pKa = 3.68LDD50 pKa = 3.88MNSNDD55 pKa = 3.37ILNAGVIKK63 pKa = 10.38AKK65 pKa = 10.34RR66 pKa = 11.84VIADD70 pKa = 3.63SIDD73 pKa = 3.47NNTEE77 pKa = 3.74SLIDD81 pKa = 3.89GGVSDD86 pKa = 4.72NEE88 pKa = 4.03IVKK91 pKa = 9.97WDD93 pKa = 3.29VDD95 pKa = 3.49TSKK98 pKa = 10.88FVGTGVFSNSEE109 pKa = 4.09GEE111 pKa = 4.4LDD113 pKa = 3.61VSPNSVSVGVHH124 pKa = 6.6KK125 pKa = 10.43IGSMGEE131 pKa = 3.8NVGFFNKK138 pKa = 9.09ATGKK142 pKa = 10.49SYY144 pKa = 10.17FPSWQGVGISDD155 pKa = 3.88SDD157 pKa = 3.73TFTAVTRR164 pKa = 11.84VHH166 pKa = 6.83GPLEE170 pKa = 4.23YY171 pKa = 10.39VVRR174 pKa = 11.84TTNVSEE180 pKa = 4.06VLINPEE186 pKa = 4.21FIVTVPYY193 pKa = 10.7SEE195 pKa = 4.43TVFKK199 pKa = 10.8IGLYY203 pKa = 8.39CTNEE207 pKa = 3.79VPNVHH212 pKa = 6.86FRR214 pKa = 11.84LTYY217 pKa = 9.68NNSIVFDD224 pKa = 4.03GDD226 pKa = 3.33MGSSQSVGMTTFTLPTPVDD245 pKa = 3.78LRR247 pKa = 11.84QGAVLVAKK255 pKa = 10.3AYY257 pKa = 10.52GIGQDD262 pKa = 4.11LYY264 pKa = 11.79VKK266 pKa = 10.26GGSDD270 pKa = 3.73GVPAYY275 pKa = 10.04SLWMRR280 pKa = 11.84PWVDD284 pKa = 3.24KK285 pKa = 10.99EE286 pKa = 4.04LATVEE291 pKa = 4.24YY292 pKa = 11.03VDD294 pKa = 5.28GKK296 pKa = 9.69PQGKK300 pKa = 7.68EE301 pKa = 3.9TFVEE305 pKa = 5.4LLDD308 pKa = 4.71CPNTYY313 pKa = 10.24VGQGKK318 pKa = 9.76KK319 pKa = 9.86LVAVNEE325 pKa = 4.38SEE327 pKa = 4.38SALEE331 pKa = 4.57FIPHH335 pKa = 5.63TLEE338 pKa = 4.26SLDD341 pKa = 3.44NVLIVSPIQTDD352 pKa = 4.35GEE354 pKa = 4.32VLMYY358 pKa = 11.08NLTEE362 pKa = 4.28NRR364 pKa = 11.84WEE366 pKa = 4.07NQALPEE372 pKa = 3.97FQEE375 pKa = 4.4DD376 pKa = 4.22IIRR379 pKa = 11.84ISYY382 pKa = 6.69TTPNSLPAKK391 pKa = 9.85QLLSTSNVGSVITFNVTEE409 pKa = 4.05IASDD413 pKa = 4.34FVQTDD418 pKa = 3.6LTSGVLTFVDD428 pKa = 3.5SAKK431 pKa = 11.25AMFTMSVQVIRR442 pKa = 11.84EE443 pKa = 3.95TGGAGDD449 pKa = 5.05ALWGMYY455 pKa = 10.2VEE457 pKa = 4.74TSMDD461 pKa = 4.33GISWSPVSGTTRR473 pKa = 11.84LLLLTGGDD481 pKa = 3.66SGDD484 pKa = 3.53VKK486 pKa = 11.08LIDD489 pKa = 3.63YY490 pKa = 7.38TVPISVVAGEE500 pKa = 4.22KK501 pKa = 10.59VRR503 pKa = 11.84FKK505 pKa = 10.98QYY507 pKa = 10.65TSDD510 pKa = 3.2SSKK513 pKa = 10.89QIGIISSPQTIAGIPTNAGVTLGVYY538 pKa = 9.72RR539 pKa = 11.84VQHH542 pKa = 5.67

Molecular weight: 58.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XUJ4|A0A6G6XUJ4_9CAUD MazG domain-containing protein OS=Vibrio phage Saratov-12 OX=2712943 GN=Saratov12_00075 PE=4 SV=1
MM1 pKa = 7.82KK2 pKa = 10.23KK3 pKa = 10.13RR4 pKa = 11.84NPNRR8 pKa = 11.84AHH10 pKa = 5.68SPEE13 pKa = 3.78RR14 pKa = 11.84LMLRR18 pKa = 11.84KK19 pKa = 8.35QTADD23 pKa = 2.91VGLIKK28 pKa = 10.53VVNLISRR35 pKa = 11.84ATGGAVLGGRR45 pKa = 11.84LGFICAAIKK54 pKa = 10.24QDD56 pKa = 3.25GANIRR61 pKa = 11.84SLSTWEE67 pKa = 4.86AYY69 pKa = 9.18GQVYY73 pKa = 10.82DD74 pKa = 4.53SVTTMAIIHH83 pKa = 6.32

Molecular weight: 9.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

12364

46

1416

301.6

33.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.225 ± 0.478

0.922 ± 0.227

6.398 ± 0.344

6.729 ± 0.367

3.664 ± 0.232

8.031 ± 0.767

1.432 ± 0.216

5.718 ± 0.209

6.082 ± 0.372

7.15 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.279

4.926 ± 0.227

3.915 ± 0.383

4.44 ± 0.403

4.958 ± 0.277

6.317 ± 0.344

6.309 ± 0.426

6.956 ± 0.308

1.407 ± 0.109

3.632 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski