Firmicutes bacterium CAG:95

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2767 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7N703|R7N703_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:95 OX=1262988 GN=BN816_00074 PE=4 SV=1
MM1 pKa = 7.88IFIHH5 pKa = 6.21NFHH8 pKa = 7.13KK9 pKa = 10.93FPGKK13 pKa = 10.17RR14 pKa = 11.84FLFPVLLLTLVLTGCGKK31 pKa = 10.46DD32 pKa = 3.21KK33 pKa = 11.11AVEE36 pKa = 3.97NYY38 pKa = 8.33RR39 pKa = 11.84ANMTQFFEE47 pKa = 4.65NIQTINDD54 pKa = 5.2AINQLDD60 pKa = 4.03PNDD63 pKa = 4.18EE64 pKa = 4.25NVGDD68 pKa = 3.92QLLPLLDD75 pKa = 4.4SLDD78 pKa = 3.72KK79 pKa = 11.25SFSQMASLEE88 pKa = 4.35VPDD91 pKa = 5.0GFPGVADD98 pKa = 4.54LAADD102 pKa = 3.52ASKK105 pKa = 11.48YY106 pKa = 5.61MTEE109 pKa = 4.2AVSCYY114 pKa = 8.26HH115 pKa = 5.32QAYY118 pKa = 10.38SGEE121 pKa = 4.53EE122 pKa = 3.96YY123 pKa = 10.67DD124 pKa = 4.53KK125 pKa = 11.19TSADD129 pKa = 2.89IAYY132 pKa = 10.41QNYY135 pKa = 8.62QFANKK140 pKa = 9.03RR141 pKa = 11.84LQYY144 pKa = 10.01IVQILHH150 pKa = 6.51GDD152 pKa = 3.14IPEE155 pKa = 4.56EE156 pKa = 3.71IFTYY160 pKa = 10.73DD161 pKa = 3.72EE162 pKa = 5.05DD163 pKa = 5.53DD164 pKa = 3.79STGSTDD170 pKa = 3.72SSADD174 pKa = 3.58DD175 pKa = 3.67FTEE178 pKa = 5.5DD179 pKa = 4.42DD180 pKa = 4.09TSTDD184 pKa = 3.62SADD187 pKa = 3.57DD188 pKa = 4.17AYY190 pKa = 10.5EE191 pKa = 4.28DD192 pKa = 4.48SEE194 pKa = 4.71DD195 pKa = 3.91SSEE198 pKa = 4.13EE199 pKa = 4.13DD200 pKa = 2.88EE201 pKa = 4.5FAYY204 pKa = 10.61GDD206 pKa = 3.66VPGTSTEE213 pKa = 4.27DD214 pKa = 3.11SAEE217 pKa = 4.14ASDD220 pKa = 4.2EE221 pKa = 4.3EE222 pKa = 4.73TVEE225 pKa = 4.02

Molecular weight: 25.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7N6R7|R7N6R7_9FIRM Xylulose kinase OS=Firmicutes bacterium CAG:95 OX=1262988 GN=xylB PE=3 SV=1
MM1 pKa = 7.73EE2 pKa = 4.93MLQIATVTLIVTQIAIAILTVMVIQTATVIPTRR35 pKa = 11.84IRR37 pKa = 11.84IPTQTRR43 pKa = 11.84IRR45 pKa = 11.84IRR47 pKa = 11.84IPIRR51 pKa = 11.84IQIQTQTQIPTRR63 pKa = 11.84IQTQTQTQTRR73 pKa = 11.84IQILIWIPTQTQIPTPTRR91 pKa = 11.84ILIRR95 pKa = 11.84IQIQIQIQTRR105 pKa = 11.84IWILTLTRR113 pKa = 11.84ILIQTQIPTRR123 pKa = 11.84IQTQTPTRR131 pKa = 11.84ILIQIPTRR139 pKa = 11.84IRR141 pKa = 11.84IRR143 pKa = 11.84IQIQIRR149 pKa = 11.84IRR151 pKa = 11.84TQIQTATQTLRR162 pKa = 11.84QTTRR166 pKa = 11.84AMIPII171 pKa = 4.48

Molecular weight: 20.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2767

0

2767

879927

29

2994

318.0

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.221 ± 0.05

1.516 ± 0.014

5.628 ± 0.04

7.864 ± 0.055

4.13 ± 0.035

6.933 ± 0.042

1.838 ± 0.021

7.353 ± 0.039

6.499 ± 0.038

9.099 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.21 ± 0.021

4.319 ± 0.033

3.288 ± 0.029

3.506 ± 0.028

4.447 ± 0.029

5.649 ± 0.034

5.333 ± 0.034

6.866 ± 0.035

0.952 ± 0.017

4.348 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski