Brevundimonas sp. Leaf363

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Brevundimonas; unclassified Brevundimonas

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2955 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5V189|A0A0Q5V189_9CAUL Uncharacterized protein OS=Brevundimonas sp. Leaf363 OX=1736353 GN=ASG17_04540 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.49CIGLLIGAMALAAPGAVLAQDD23 pKa = 3.48EE24 pKa = 4.92GDD26 pKa = 3.31WSVEE30 pKa = 3.7ATAGAVSDD38 pKa = 3.64YY39 pKa = 10.73RR40 pKa = 11.84FRR42 pKa = 11.84GLSLSGGDD50 pKa = 3.65PALQAGVTVSHH61 pKa = 7.0ASGFYY66 pKa = 10.25ADD68 pKa = 4.75VYY70 pKa = 10.67LSSIEE75 pKa = 4.26TYY77 pKa = 10.85GAGDD81 pKa = 4.38DD82 pKa = 4.17GDD84 pKa = 4.18GSEE87 pKa = 5.83LEE89 pKa = 4.29VTLTGGWAGAWLGLDD104 pKa = 2.8WDD106 pKa = 4.66AAVSAYY112 pKa = 10.07RR113 pKa = 11.84YY114 pKa = 9.7PGGDD118 pKa = 3.11DD119 pKa = 3.04VDD121 pKa = 4.16YY122 pKa = 11.84VEE124 pKa = 5.65VPVQASRR131 pKa = 11.84TFGPATATLGVAYY144 pKa = 10.2APAQRR149 pKa = 11.84ALGEE153 pKa = 3.94EE154 pKa = 4.31DD155 pKa = 5.23NRR157 pKa = 11.84YY158 pKa = 9.31AWAGLDD164 pKa = 3.78YY165 pKa = 10.93APEE168 pKa = 4.15AWPVSLAASIGYY180 pKa = 9.71EE181 pKa = 4.05DD182 pKa = 5.3GAWAPDD188 pKa = 3.28GKK190 pKa = 9.33TDD192 pKa = 3.1WRR194 pKa = 11.84IGAFLPLGRR203 pKa = 11.84LTAGVEE209 pKa = 4.43WIDD212 pKa = 3.71SDD214 pKa = 4.21ADD216 pKa = 3.4TGALVGSVFASFF228 pKa = 3.92

Molecular weight: 23.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5VLS4|A0A0Q5VLS4_9CAUL Uncharacterized protein OS=Brevundimonas sp. Leaf363 OX=1736353 GN=ASG17_02005 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.43NGAKK29 pKa = 9.48IVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2955

0

2955

935029

41

1628

316.4

34.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.024 ± 0.072

0.692 ± 0.011

6.185 ± 0.04

5.468 ± 0.039

3.471 ± 0.028

9.082 ± 0.039

1.808 ± 0.021

4.392 ± 0.029

2.685 ± 0.035

9.745 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.02

2.261 ± 0.027

5.47 ± 0.036

3.214 ± 0.025

7.5 ± 0.04

5.014 ± 0.03

5.353 ± 0.036

7.659 ± 0.034

1.53 ± 0.023

2.125 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski