Gemycircularvirus HV-GcV2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus humas5; Human associated gemykibivirus 5

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A193CE78|A0A193CE78_9VIRU Capsid protein OS=Gemycircularvirus HV-GcV2 OX=1862825 GN=cap PE=4 SV=1
MM1 pKa = 7.89LGEE4 pKa = 5.09LGAEE8 pKa = 4.07CIVGRR13 pKa = 11.84EE14 pKa = 3.97SHH16 pKa = 6.96ADD18 pKa = 3.35GGLHH22 pKa = 5.98LHH24 pKa = 6.48AFFMFEE30 pKa = 4.19RR31 pKa = 11.84KK32 pKa = 9.23FEE34 pKa = 4.26SRR36 pKa = 11.84DD37 pKa = 3.27VRR39 pKa = 11.84VFDD42 pKa = 4.91VDD44 pKa = 3.72GLHH47 pKa = 6.49PNIVRR52 pKa = 11.84GYY54 pKa = 4.45TTPRR58 pKa = 11.84KK59 pKa = 9.59GADD62 pKa = 3.33YY63 pKa = 10.49AIKK66 pKa = 10.86DD67 pKa = 3.61GDD69 pKa = 4.09VVAGGLDD76 pKa = 3.47ISQLGAPVSHH86 pKa = 6.97TSAVWATIIMASTRR100 pKa = 11.84EE101 pKa = 4.06EE102 pKa = 4.09FFEE105 pKa = 4.68ACATLAPRR113 pKa = 11.84SLVCSFTSLKK123 pKa = 10.62CYY125 pKa = 10.61ADD127 pKa = 2.9WKK129 pKa = 10.1YY130 pKa = 10.83RR131 pKa = 11.84PEE133 pKa = 4.19PVPYY137 pKa = 9.09EE138 pKa = 4.27HH139 pKa = 7.71PSGLSFDD146 pKa = 3.63TTAYY150 pKa = 9.77PEE152 pKa = 3.99LDD154 pKa = 2.93AWVSHH159 pKa = 5.92ALEE162 pKa = 4.66GPEE165 pKa = 5.0CGGEE169 pKa = 4.1CPPTAEE175 pKa = 4.47DD176 pKa = 3.91TFPSLRR182 pKa = 11.84PPGALRR188 pKa = 11.84VYY190 pKa = 11.12AMSHH194 pKa = 6.03CWAAWRR200 pKa = 11.84LPAKK204 pKa = 10.6LMGVMLTYY212 pKa = 10.36SGRR215 pKa = 11.84RR216 pKa = 11.84RR217 pKa = 11.84SLVLYY222 pKa = 10.58GPTRR226 pKa = 11.84LGKK229 pKa = 7.65TLWARR234 pKa = 11.84ALGSHH239 pKa = 7.14AYY241 pKa = 10.15FGGLFSLDD249 pKa = 3.47EE250 pKa = 4.27SLEE253 pKa = 4.28DD254 pKa = 2.83VDD256 pKa = 5.06YY257 pKa = 11.57AVFDD261 pKa = 4.3DD262 pKa = 4.09MQGGLKK268 pKa = 10.02FFHH271 pKa = 7.28AYY273 pKa = 9.59KK274 pKa = 10.26FWLGAQSQFWATDD287 pKa = 3.02KK288 pKa = 11.5YY289 pKa = 10.22KK290 pKa = 10.83GKK292 pKa = 10.54RR293 pKa = 11.84LIHH296 pKa = 6.0WGKK299 pKa = 9.27PSIYY303 pKa = 9.39IANGNPLTDD312 pKa = 3.55EE313 pKa = 4.88GVDD316 pKa = 3.87HH317 pKa = 7.71DD318 pKa = 4.77WLLGNCDD325 pKa = 3.46FVEE328 pKa = 4.31ITTSLLVPEE337 pKa = 4.86VGPVTLEE344 pKa = 3.85VV345 pKa = 3.38

Molecular weight: 38.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A193CE78|A0A193CE78_9VIRU Capsid protein OS=Gemycircularvirus HV-GcV2 OX=1862825 GN=cap PE=4 SV=1
MM1 pKa = 7.15SVRR4 pKa = 11.84RR5 pKa = 11.84ILNKK9 pKa = 9.59TSQKK13 pKa = 10.43KK14 pKa = 9.89RR15 pKa = 11.84DD16 pKa = 3.83TLLTWSNVDD25 pKa = 3.86PSNPDD30 pKa = 3.55PDD32 pKa = 4.01NDD34 pKa = 4.01TYY36 pKa = 10.9TPSGATIPGGTGAFQYY52 pKa = 11.0MMFCPTARR60 pKa = 11.84GLEE63 pKa = 4.29SFTGSLGSKK72 pKa = 10.37ANTTIRR78 pKa = 11.84TSTTVFARR86 pKa = 11.84GYY88 pKa = 10.93KK89 pKa = 10.16EE90 pKa = 3.47ITQLRR95 pKa = 11.84TRR97 pKa = 11.84SGKK100 pKa = 7.9GWQWRR105 pKa = 11.84RR106 pKa = 11.84ICFTLKK112 pKa = 10.52GDD114 pKa = 4.25SLNEE118 pKa = 4.12GNSDD122 pKa = 3.78PASSPVFRR130 pKa = 11.84EE131 pKa = 3.74TSNGYY136 pKa = 9.02QRR138 pKa = 11.84LIKK141 pKa = 10.27IADD144 pKa = 3.96PDD146 pKa = 3.77RR147 pKa = 11.84VSTLLFRR154 pKa = 11.84GNNGQDD160 pKa = 3.06WNDD163 pKa = 3.28PTMASLDD170 pKa = 3.65TRR172 pKa = 11.84RR173 pKa = 11.84VSVKK177 pKa = 9.86YY178 pKa = 10.39DD179 pKa = 3.5RR180 pKa = 11.84LRR182 pKa = 11.84MIRR185 pKa = 11.84SGNDD189 pKa = 3.02DD190 pKa = 3.55GVVRR194 pKa = 11.84TYY196 pKa = 10.81KK197 pKa = 10.06QWFPMNKK204 pKa = 9.43NIYY207 pKa = 10.01YY208 pKa = 10.17EE209 pKa = 4.46DD210 pKa = 4.9DD211 pKa = 3.73EE212 pKa = 5.52SGSTMNTNPFSVEE225 pKa = 4.18SNQGMGDD232 pKa = 3.75YY233 pKa = 10.68YY234 pKa = 10.44IVDD237 pKa = 3.95IFRR240 pKa = 11.84ANPDD244 pKa = 3.13TTAEE248 pKa = 4.12DD249 pKa = 3.77SLDD252 pKa = 3.8FEE254 pKa = 5.95CNGTYY259 pKa = 9.97FWHH262 pKa = 6.74EE263 pKa = 4.0KK264 pKa = 10.03

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

663

54

345

221.0

24.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.486 ± 1.808

1.659 ± 0.533

7.391 ± 0.766

4.827 ± 1.001

4.676 ± 0.625

9.653 ± 1.742

2.262 ± 0.976

3.469 ± 0.41

4.072 ± 0.245

7.994 ± 1.55

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.715 ± 0.684

3.469 ± 1.922

5.279 ± 0.817

1.961 ± 0.588

6.335 ± 0.854

7.089 ± 1.235

8.446 ± 2.66

6.033 ± 1.174

2.413 ± 0.542

3.771 ± 0.738
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski