Gordonia phage Woes

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Woesvirus; Gordonia virus Woes

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160DDP4|A0A160DDP4_9CAUD Uncharacterized protein OS=Gordonia phage Woes OX=1838084 GN=91 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 4.54VGRR5 pKa = 11.84LMQGPPYY12 pKa = 9.94YY13 pKa = 10.58AGPEE17 pKa = 3.77EE18 pKa = 4.42LGYY21 pKa = 8.86YY22 pKa = 8.06TYY24 pKa = 10.35PDD26 pKa = 3.34SDD28 pKa = 3.95MDD30 pKa = 3.71EE31 pKa = 3.78LAYY34 pKa = 10.42RR35 pKa = 11.84IRR37 pKa = 11.84SRR39 pKa = 11.84LIPIWDD45 pKa = 3.8EE46 pKa = 4.39LEE48 pKa = 4.32VPLLPEE54 pKa = 3.8EE55 pKa = 4.3EE56 pKa = 4.73EE57 pKa = 3.94IVEE60 pKa = 4.09ATEE63 pKa = 4.01FF64 pKa = 3.55

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166Y5R5|A0A166Y5R5_9CAUD 4Fe-4S Wbl-type domain-containing protein OS=Gordonia phage Woes OX=1838084 GN=42 PE=4 SV=1
MM1 pKa = 7.91KK2 pKa = 10.28INSWKK7 pKa = 9.8NKK9 pKa = 8.78KK10 pKa = 10.12VNLTKK15 pKa = 10.37RR16 pKa = 11.84RR17 pKa = 11.84PRR19 pKa = 11.84HH20 pKa = 4.37AAEE23 pKa = 4.46YY24 pKa = 8.59GTEE27 pKa = 3.94AVPTFAYY34 pKa = 9.5PHH36 pKa = 6.15AQFIIAQLQQHH47 pKa = 5.52QPIQQHH53 pKa = 3.76QPIRR57 pKa = 11.84RR58 pKa = 11.84TAA60 pKa = 2.97

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

22784

33

3322

250.4

27.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.915 ± 1.06

0.948 ± 0.157

6.031 ± 0.348

7.022 ± 0.416

3.524 ± 0.117

7.834 ± 0.307

1.659 ± 0.171

5.346 ± 0.206

5.1 ± 0.235

7.273 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.143

4.082 ± 0.156

5.201 ± 0.381

3.691 ± 0.25

5.728 ± 0.407

6.197 ± 0.19

6.092 ± 0.263

7.15 ± 0.196

1.729 ± 0.129

3.037 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski