Klebsiella phage vB_KpnP_NahiliMali

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Ningirsuvirus; unclassified Ningirsuvirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NI73|A0A5B9NI73_9CAUD Putative tRNA nucleotidyltransferase OS=Klebsiella phage vB_KpnP_NahiliMali OX=2591373 GN=NAHI_24 PE=4 SV=1
MM1 pKa = 7.32SALHH5 pKa = 5.62VAYY8 pKa = 9.51IIAIILTIVLAWALNDD24 pKa = 5.58DD25 pKa = 5.13DD26 pKa = 6.8DD27 pKa = 6.41DD28 pKa = 6.23DD29 pKa = 6.24SGGPPTGHH37 pKa = 6.32ATHH40 pKa = 6.51YY41 pKa = 10.85

Molecular weight: 4.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9NMP0|A0A5B9NMP0_9CAUD Putative DNA primase/helicase OS=Klebsiella phage vB_KpnP_NahiliMali OX=2591373 GN=NAHI_25 PE=4 SV=1
MM1 pKa = 7.74AKK3 pKa = 9.32PHH5 pKa = 5.99NFRR8 pKa = 11.84VVHH11 pKa = 6.53RR12 pKa = 11.84YY13 pKa = 9.18GVTTTPRR20 pKa = 11.84VIQDD24 pKa = 3.67LNTGRR29 pKa = 11.84LHH31 pKa = 6.95PLKK34 pKa = 10.48QQTPHH39 pKa = 6.31IAKK42 pKa = 10.39AGGLTFHH49 pKa = 7.06IMKK52 pKa = 10.3AHH54 pKa = 6.72TGTSNHH60 pKa = 5.3VVVKK64 pKa = 9.51PYY66 pKa = 10.34EE67 pKa = 4.55GNWPRR72 pKa = 11.84LMFYY76 pKa = 10.2VKK78 pKa = 10.42RR79 pKa = 11.84GLEE82 pKa = 3.95LFAA85 pKa = 6.87

Molecular weight: 9.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12353

40

1324

242.2

26.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.212 ± 0.463

0.81 ± 0.126

6.428 ± 0.269

6.565 ± 0.289

3.716 ± 0.206

7.456 ± 0.27

1.935 ± 0.178

5.14 ± 0.216

6.517 ± 0.336

8.03 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.93 ± 0.184

4.736 ± 0.227

3.497 ± 0.202

4.42 ± 0.4

5.205 ± 0.151

6.031 ± 0.331

5.829 ± 0.28

6.768 ± 0.316

1.409 ± 0.168

3.368 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski