Agromyces sp. MMS17-SY077

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agromyces; unclassified Agromyces

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3503 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I4NZX8|A0A6I4NZX8_9MICO Uncharacterized protein OS=Agromyces sp. MMS17-SY077 OX=2662446 GN=GB864_09465 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.5SKK4 pKa = 10.76LLATAGVVALSAAALTGCSTASGGDD29 pKa = 3.57SAKK32 pKa = 10.65ADD34 pKa = 3.71CTNEE38 pKa = 3.71IVNPDD43 pKa = 3.3ATQVSVWAWYY53 pKa = 8.14PAFEE57 pKa = 4.24EE58 pKa = 4.65VVDD61 pKa = 5.01LFNEE65 pKa = 4.14SHH67 pKa = 7.85DD68 pKa = 4.13DD69 pKa = 4.6VQICWTNAGQGNDD82 pKa = 3.61EE83 pKa = 4.1YY84 pKa = 10.83TKK86 pKa = 10.76FSTAIEE92 pKa = 4.47AGSGAPDD99 pKa = 3.59VIMLEE104 pKa = 4.27SEE106 pKa = 4.38VLSSFSIRR114 pKa = 11.84KK115 pKa = 9.16ALVDD119 pKa = 3.6LTEE122 pKa = 4.18YY123 pKa = 10.9GAADD127 pKa = 3.58VQDD130 pKa = 5.45DD131 pKa = 4.21YY132 pKa = 12.0TAGAWKK138 pKa = 10.3DD139 pKa = 3.79VSSGDD144 pKa = 3.36AVYY147 pKa = 10.34AIPVDD152 pKa = 4.16GGPMGMLYY160 pKa = 10.4RR161 pKa = 11.84KK162 pKa = 10.15DD163 pKa = 4.04ILDD166 pKa = 3.37QYY168 pKa = 11.01GIAAPTTWDD177 pKa = 3.37EE178 pKa = 4.43FKK180 pKa = 11.05AAAEE184 pKa = 3.95ALKK187 pKa = 10.7AAGAPGVLADD197 pKa = 4.64FPTNGRR203 pKa = 11.84AYY205 pKa = 10.15NQALFAQAGSVPFVYY220 pKa = 10.67DD221 pKa = 3.23NANPTEE227 pKa = 3.96IGIDD231 pKa = 3.73VNDD234 pKa = 4.04EE235 pKa = 3.73GSKK238 pKa = 10.39QVLSYY243 pKa = 10.46WNEE246 pKa = 3.67LVQDD250 pKa = 4.14GLVATDD256 pKa = 4.18DD257 pKa = 4.86AFTADD262 pKa = 4.2YY263 pKa = 7.76NTKK266 pKa = 10.53LVDD269 pKa = 3.71GSYY272 pKa = 10.7AIYY275 pKa = 10.3VAAAWGPGYY284 pKa = 10.88LSGLADD290 pKa = 4.16ADD292 pKa = 4.77SDD294 pKa = 4.57AEE296 pKa = 4.23WVAAPVPQWDD306 pKa = 3.61AANPVQINWGGSTFAVTSQAKK327 pKa = 10.07DD328 pKa = 3.06KK329 pKa = 10.68EE330 pKa = 4.28AAATVAKK337 pKa = 10.04EE338 pKa = 3.98VFGTEE343 pKa = 4.19EE344 pKa = 3.41AWKK347 pKa = 10.41IGIEE351 pKa = 3.97QGALFPLWKK360 pKa = 9.9PILEE364 pKa = 4.04SDD366 pKa = 3.72YY367 pKa = 10.99FRR369 pKa = 11.84DD370 pKa = 3.23LEE372 pKa = 4.32YY373 pKa = 10.45PFFGGQQINKK383 pKa = 9.86DD384 pKa = 3.63VFLAAAAGYY393 pKa = 10.22EE394 pKa = 4.24GFTFSPFQNYY404 pKa = 10.17AYY406 pKa = 10.31DD407 pKa = 3.62QLTEE411 pKa = 3.67QLYY414 pKa = 11.37AMVQGEE420 pKa = 4.35KK421 pKa = 10.66SSDD424 pKa = 3.38QALDD428 pKa = 3.79DD429 pKa = 4.31LQASLEE435 pKa = 4.2QYY437 pKa = 10.69AGEE440 pKa = 4.72QGFTLQQ446 pKa = 4.51

Molecular weight: 47.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I4P455|A0A6I4P455_9MICO Flagellar protein FlgN OS=Agromyces sp. MMS17-SY077 OX=2662446 GN=GB864_06500 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3503

0

3503

1161124

29

3243

331.5

35.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.62 ± 0.066

0.444 ± 0.009

6.31 ± 0.042

5.866 ± 0.044

3.187 ± 0.024

9.449 ± 0.032

1.837 ± 0.022

4.212 ± 0.031

1.699 ± 0.029

10.302 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.512 ± 0.016

1.773 ± 0.024

5.627 ± 0.033

2.43 ± 0.025

7.369 ± 0.053

5.21 ± 0.035

5.625 ± 0.046

9.058 ± 0.039

1.543 ± 0.02

1.924 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski