Lactobacillus phage P1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Tybeckvirinae; Maenadvirus; Lactobacillus virus P1

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RCT2|A0A1S5RCT2_9CAUD Uncharacterized protein OS=Lactobacillus phage P1 OX=1846168 GN=LVP1_g043 PE=4 SV=1
MM1 pKa = 7.76DD2 pKa = 4.07TLINILTFLLVAALMVVFYY21 pKa = 10.46GIVAAVLGALWGFNVSLALALRR43 pKa = 11.84AGVTGYY49 pKa = 9.97ILSIVAPMIFCSPSSDD65 pKa = 3.36EE66 pKa = 4.09

Molecular weight: 6.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RCS0|A0A1S5RCS0_9CAUD PemK family transcriptional regulator OS=Lactobacillus phage P1 OX=1846168 GN=LVP1_g038 PE=4 SV=1
MM1 pKa = 7.25VCFLHH6 pKa = 6.86RR7 pKa = 11.84KK8 pKa = 8.79KK9 pKa = 10.89KK10 pKa = 10.21EE11 pKa = 3.82GYY13 pKa = 9.49LYY15 pKa = 10.33EE16 pKa = 4.54GNFRR20 pKa = 11.84RR21 pKa = 11.84TTIKK25 pKa = 10.41RR26 pKa = 11.84LKK28 pKa = 10.08DD29 pKa = 3.36LKK31 pKa = 10.91RR32 pKa = 11.84PNTQIKK38 pKa = 10.22KK39 pKa = 9.54MYY41 pKa = 10.24FLAA44 pKa = 5.55

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

20139

38

1887

234.2

26.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.828 ± 0.507

0.69 ± 0.105

6.981 ± 0.457

6.003 ± 0.329

3.883 ± 0.182

6.932 ± 0.454

1.817 ± 0.144

6.296 ± 0.193

8.069 ± 0.357

8.178 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.304 ± 0.097

6.103 ± 0.217

2.741 ± 0.157

3.62 ± 0.199

3.863 ± 0.216

7.051 ± 0.333

6.356 ± 0.315

6.609 ± 0.266

1.226 ± 0.119

4.449 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski