Erwinia phage Hena1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Henunavirus; Erwinia virus Hena1

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9J5Z4|A0A6B9J5Z4_9CAUD Uncharacterized protein OS=Erwinia phage Hena1 OX=2678601 GN=Hena1_01850 PE=4 SV=1
MM1 pKa = 7.77AYY3 pKa = 9.38ISYY6 pKa = 8.45TWDD9 pKa = 3.28LDD11 pKa = 3.88GYY13 pKa = 9.11PDD15 pKa = 3.17QTMRR19 pKa = 11.84VVLDD23 pKa = 3.4SQTYY27 pKa = 8.08EE28 pKa = 5.15MRR30 pKa = 11.84FQWNEE35 pKa = 3.5RR36 pKa = 11.84DD37 pKa = 3.99EE38 pKa = 4.31SWLVYY43 pKa = 10.44FGDD46 pKa = 3.81VGSDD50 pKa = 3.22PTISFKK56 pKa = 8.71MTAFVDD62 pKa = 4.65FFQPYY67 pKa = 9.7QYY69 pKa = 10.95LDD71 pKa = 4.22NIPSGKK77 pKa = 10.45LMVVSYY83 pKa = 10.05TEE85 pKa = 3.67YY86 pKa = 9.79KK87 pKa = 10.33KK88 pKa = 10.78RR89 pKa = 11.84VGRR92 pKa = 11.84YY93 pKa = 9.24NIGFLSDD100 pKa = 3.69LQLMYY105 pKa = 10.75VSPEE109 pKa = 3.91DD110 pKa = 3.66EE111 pKa = 5.45DD112 pKa = 4.12IEE114 pKa = 4.46EE115 pKa = 4.2EE116 pKa = 4.16

Molecular weight: 13.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9JC92|A0A6B9JC92_9CAUD Uncharacterized protein OS=Erwinia phage Hena1 OX=2678601 GN=Hena1_00560 PE=4 SV=1
MM1 pKa = 7.78SKK3 pKa = 9.04RR4 pKa = 11.84TLRR7 pKa = 11.84KK8 pKa = 9.58AYY10 pKa = 9.96QKK12 pKa = 10.02EE13 pKa = 3.51LAMALRR19 pKa = 11.84TTKK22 pKa = 10.34RR23 pKa = 11.84GRR25 pKa = 11.84KK26 pKa = 8.7DD27 pKa = 2.96RR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 3.81NDD32 pKa = 3.09NSFVAAGEE40 pKa = 3.94RR41 pKa = 11.84DD42 pKa = 3.13GWEE45 pKa = 4.0PTNGG49 pKa = 3.33

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

240

0

240

43832

30

1297

182.6

20.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.12 ± 0.214

1.129 ± 0.08

6.356 ± 0.129

6.445 ± 0.218

4.18 ± 0.101

7.385 ± 0.26

1.944 ± 0.099

5.658 ± 0.131

6.71 ± 0.198

8.044 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.847 ± 0.092

4.67 ± 0.132

3.714 ± 0.124

3.456 ± 0.127

4.809 ± 0.143

5.856 ± 0.146

6.283 ± 0.274

7.054 ± 0.147

1.606 ± 0.092

3.732 ± 0.12
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski