Prevotella sp. CAG:5226

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2309 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5ZZC4|R5ZZC4_9BACT Uncharacterized protein OS=Prevotella sp. CAG:5226 OX=1262930 GN=BN693_01657 PE=4 SV=1
MM1 pKa = 7.41TGIFYY6 pKa = 10.71GSTTGCTEE14 pKa = 3.69NVANAIAQQLGVEE27 pKa = 4.58AKK29 pKa = 10.45DD30 pKa = 3.39IYY32 pKa = 11.59NVGTTDD38 pKa = 3.22ANKK41 pKa = 10.64ALDD44 pKa = 4.14YY45 pKa = 8.68DD46 pKa = 4.15TLLLGSSTWGCGDD59 pKa = 5.17LQDD62 pKa = 4.66DD63 pKa = 4.58WYY65 pKa = 10.84DD66 pKa = 3.81FLDD69 pKa = 4.0TLSSLDD75 pKa = 3.59LTGKK79 pKa = 10.04RR80 pKa = 11.84VGIFGCGDD88 pKa = 3.26SDD90 pKa = 4.11SYY92 pKa = 12.12SDD94 pKa = 3.75TFCGAIAQIYY104 pKa = 10.32DD105 pKa = 3.33GLQNTGCTFIGAFEE119 pKa = 4.07PTDD122 pKa = 3.78YY123 pKa = 11.3NVTDD127 pKa = 3.73SDD129 pKa = 3.86VCRR132 pKa = 11.84DD133 pKa = 3.41GKK135 pKa = 10.85FVGLAIDD142 pKa = 4.06EE143 pKa = 4.83GDD145 pKa = 4.98DD146 pKa = 3.44EE147 pKa = 5.13GKK149 pKa = 9.41NQARR153 pKa = 11.84IEE155 pKa = 4.14AWCSLVKK162 pKa = 10.77

Molecular weight: 17.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6ABB6|R6ABB6_9BACT Uncharacterized protein OS=Prevotella sp. CAG:5226 OX=1262930 GN=BN693_00498 PE=4 SV=1
MM1 pKa = 6.68VHH3 pKa = 6.44HH4 pKa = 6.82RR5 pKa = 11.84QNGLRR10 pKa = 11.84AQPHH14 pKa = 5.74PRR16 pKa = 11.84AVRR19 pKa = 11.84PRR21 pKa = 11.84LPNQPRR27 pKa = 11.84LPHH30 pKa = 6.17EE31 pKa = 4.11PQRR34 pKa = 11.84PHH36 pKa = 5.67RR37 pKa = 11.84HH38 pKa = 5.07HH39 pKa = 7.02RR40 pKa = 11.84RR41 pKa = 11.84LLAIPRR47 pKa = 11.84HH48 pKa = 5.71RR49 pKa = 11.84PPLHH53 pKa = 5.57TLRR56 pKa = 11.84LAVQPTQHH64 pKa = 5.87TRR66 pKa = 11.84LLLPSRR72 pKa = 11.84ALVRR76 pKa = 11.84HH77 pKa = 5.34TLVLPRR83 pKa = 11.84LRR85 pKa = 11.84PLPHH89 pKa = 7.18RR90 pKa = 11.84LLLLHH95 pKa = 6.51TPQHH99 pKa = 5.66LHH101 pKa = 5.96HH102 pKa = 7.08LPRR105 pKa = 11.84LHH107 pKa = 6.36MAPPPSRR114 pKa = 11.84PPKK117 pKa = 9.5PLRR120 pKa = 11.84LMASQTRR127 pKa = 11.84CRR129 pKa = 11.84HH130 pKa = 4.04ARR132 pKa = 11.84IRR134 pKa = 11.84PRR136 pKa = 11.84HH137 pKa = 4.4TTATPTQPPRR147 pKa = 11.84PSQPPRR153 pKa = 11.84QQLQLARR160 pKa = 11.84PPLQQ164 pKa = 3.75

Molecular weight: 19.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2309

0

2309

790966

29

6814

342.6

38.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.524 ± 0.056

1.458 ± 0.025

5.77 ± 0.046

5.709 ± 0.061

4.115 ± 0.036

6.86 ± 0.051

2.294 ± 0.025

5.911 ± 0.051

5.955 ± 0.052

8.942 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.792 ± 0.025

4.931 ± 0.047

3.834 ± 0.026

3.912 ± 0.036

4.942 ± 0.046

5.736 ± 0.044

6.064 ± 0.046

6.821 ± 0.042

1.204 ± 0.019

4.225 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski