Ectothiorhodospiraceae bacterium 2226

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; unclassified Ectothiorhodospiraceae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2766 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N1DQ46|A0A6N1DQ46_9GAMM Malonyl-[acyl-carrier protein] O-methyltransferase OS=Ectothiorhodospiraceae bacterium 2226 OX=2740164 GN=bioC PE=3 SV=1
MM1 pKa = 7.3NRR3 pKa = 11.84TALFALAFGGLTVGAGGAAFADD25 pKa = 3.82TDD27 pKa = 3.78EE28 pKa = 4.56TATEE32 pKa = 4.41CSVSSLDD39 pKa = 3.34GSYY42 pKa = 10.89VYY44 pKa = 10.94SAMGTLAGEE53 pKa = 4.95PYY55 pKa = 10.55ASAGIMSFDD64 pKa = 3.63GDD66 pKa = 3.83GNLAILASRR75 pKa = 11.84SVEE78 pKa = 4.01RR79 pKa = 11.84EE80 pKa = 3.51QLTQSGTYY88 pKa = 10.0SIEE91 pKa = 4.53SNCSASMEE99 pKa = 4.37VADD102 pKa = 4.44GTINDD107 pKa = 5.13LYY109 pKa = 10.68LGPAGQSFKK118 pKa = 10.61FVRR121 pKa = 11.84VSGEE125 pKa = 3.9DD126 pKa = 3.43VVVGEE131 pKa = 4.7AEE133 pKa = 4.01RR134 pKa = 11.84VTTGFVGEE142 pKa = 4.36PPGPP146 pKa = 3.59

Molecular weight: 14.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N1DRC4|A0A6N1DRC4_9GAMM Probable cytosol aminopeptidase OS=Ectothiorhodospiraceae bacterium 2226 OX=2740164 GN=pepA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.41GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.99TRR25 pKa = 11.84GGRR28 pKa = 11.84LVLKK32 pKa = 10.46ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.31GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2766

0

2766

869014

37

2819

314.2

34.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.848 ± 0.07

0.847 ± 0.015

5.451 ± 0.042

6.499 ± 0.049

3.215 ± 0.031

8.54 ± 0.048

2.533 ± 0.022

3.983 ± 0.032

2.219 ± 0.038

11.23 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.021

2.196 ± 0.026

5.349 ± 0.04

3.548 ± 0.026

8.411 ± 0.05

4.289 ± 0.035

4.633 ± 0.033

8.002 ± 0.04

1.406 ± 0.021

2.602 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski