Ruegeria phage Tedan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9V0J9|A0A7G9V0J9_9CAUD Uncharacterized protein OS=Ruegeria phage Tedan OX=2759200 GN=P67b_00045 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 5.12LADD5 pKa = 3.93YY6 pKa = 10.93GEE8 pKa = 4.39PEE10 pKa = 4.43YY11 pKa = 11.25LDD13 pKa = 4.07GATAQYY19 pKa = 10.37CVRR22 pKa = 11.84ATDD25 pKa = 3.87WSWEE29 pKa = 3.95AYY31 pKa = 8.09FTEE34 pKa = 4.64EE35 pKa = 3.72EE36 pKa = 4.04DD37 pKa = 3.71AKK39 pKa = 11.42AFLALKK45 pKa = 10.56SGTEE49 pKa = 4.03VKK51 pKa = 10.67

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9V0G9|A0A7G9V0G9_9CAUD Uncharacterized protein OS=Ruegeria phage Tedan OX=2759200 GN=P67b_00015 PE=4 SV=1
MM1 pKa = 7.34TNPVRR6 pKa = 11.84IRR8 pKa = 11.84GVDD11 pKa = 3.57YY12 pKa = 10.24PSQKK16 pKa = 10.51AAADD20 pKa = 3.76ALGVSSWTIHH30 pKa = 4.98YY31 pKa = 9.48RR32 pKa = 11.84LNRR35 pKa = 11.84GTMQDD40 pKa = 3.58LGATRR45 pKa = 11.84PKK47 pKa = 9.39TGPVSAIPCIVHH59 pKa = 4.99GVRR62 pKa = 11.84YY63 pKa = 10.31KK64 pKa = 11.44SMTEE68 pKa = 3.49AKK70 pKa = 10.01KK71 pKa = 10.72VLRR74 pKa = 11.84LSNGAFEE81 pKa = 4.12TLIRR85 pKa = 11.84KK86 pKa = 9.41GNVTLLMDD94 pKa = 4.65EE95 pKa = 4.97RR96 pKa = 11.84PPLTPRR102 pKa = 3.59

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

19001

37

1140

200.0

22.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.673 ± 0.339

0.921 ± 0.117

7.052 ± 0.206

6.647 ± 0.361

3.779 ± 0.185

7.768 ± 0.208

1.774 ± 0.136

4.758 ± 0.143

4.216 ± 0.438

8.647 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.188

4.1 ± 0.271

4.626 ± 0.235

3.826 ± 0.145

6.347 ± 0.262

5.473 ± 0.263

6.879 ± 0.411

6.779 ± 0.191

1.779 ± 0.157

2.442 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski