Methylobacterium sp. (strain 4-46)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; unclassified Methylobacterium

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6647 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0UR86|B0UR86_METS4 Uncharacterized protein OS=Methylobacterium sp. (strain 4-46) OX=426117 GN=M446_6966 PE=4 SV=1
MM1 pKa = 7.46SGSGNDD7 pKa = 3.8QIAGGYY13 pKa = 10.29GNDD16 pKa = 4.77LIDD19 pKa = 4.54GGGGDD24 pKa = 5.11DD25 pKa = 4.67VIASGGNADD34 pKa = 4.44LATGRR39 pKa = 11.84DD40 pKa = 3.47TVYY43 pKa = 11.01GGGGSDD49 pKa = 3.76VIYY52 pKa = 9.74WAYY55 pKa = 9.67DD56 pKa = 3.34TTGSLLYY63 pKa = 10.89GDD65 pKa = 5.2AGDD68 pKa = 4.24GAADD72 pKa = 4.19GGNDD76 pKa = 4.04TITGGSGNDD85 pKa = 3.6TIFGEE90 pKa = 4.9GGDD93 pKa = 3.53NYY95 pKa = 10.38IRR97 pKa = 11.84GGKK100 pKa = 10.07GNDD103 pKa = 3.93AIDD106 pKa = 4.05GGSGNDD112 pKa = 3.75TIRR115 pKa = 11.84GSGGEE120 pKa = 4.18GMDD123 pKa = 3.75TVHH126 pKa = 6.74GGSGNDD132 pKa = 3.91LITYY136 pKa = 6.81TTDD139 pKa = 2.86ASSVRR144 pKa = 11.84LFGDD148 pKa = 3.59AGDD151 pKa = 4.3DD152 pKa = 4.18SIAGGYY158 pKa = 9.1GADD161 pKa = 3.57TLIGGVGNDD170 pKa = 3.8TLSGGLGADD179 pKa = 3.19SFVFGANDD187 pKa = 3.57GTDD190 pKa = 3.26VVRR193 pKa = 11.84DD194 pKa = 4.15FSVGQGDD201 pKa = 4.82KK202 pKa = 10.43IALIAGTTAEE212 pKa = 4.69KK213 pKa = 10.76SRR215 pKa = 11.84ASDD218 pKa = 4.04GFLQLNFYY226 pKa = 11.09DD227 pKa = 3.92SGHH230 pKa = 5.59VLGGSVEE237 pKa = 4.39LYY239 pKa = 9.83GVSYY243 pKa = 8.81NTNLNNVLVQAPSSMTAA260 pKa = 2.94

Molecular weight: 25.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0UFL7|B0UFL7_METS4 ATPase associated with various cellular activities AAA_3 OS=Methylobacterium sp. (strain 4-46) OX=426117 GN=M446_1201 PE=4 SV=1
MM1 pKa = 8.05RR2 pKa = 11.84ILSIAATILTVLTVLILSAAFRR24 pKa = 11.84LTLAPLRR31 pKa = 11.84AVRR34 pKa = 11.84GLKK37 pKa = 9.95LRR39 pKa = 11.84RR40 pKa = 11.84QVTGPAAPAAVRR52 pKa = 3.66

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6647

0

6647

2076946

29

4855

312.5

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.045 ± 0.057

0.833 ± 0.01

5.244 ± 0.023

5.508 ± 0.031

3.28 ± 0.018

9.232 ± 0.035

1.89 ± 0.014

4.079 ± 0.02

2.095 ± 0.023

10.821 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.927 ± 0.014

1.917 ± 0.017

6.14 ± 0.034

2.705 ± 0.019

8.779 ± 0.038

4.737 ± 0.028

4.975 ± 0.032

7.569 ± 0.022

1.259 ± 0.012

1.965 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski