Gordonia phage Wocket

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V4G4|A0A649V4G4_9CAUD Scaffolding protein OS=Gordonia phage Wocket OX=2656543 GN=5 PE=4 SV=1
MM1 pKa = 8.76IMIDD5 pKa = 3.54PHH7 pKa = 7.9DD8 pKa = 3.82VADD11 pKa = 5.81FYY13 pKa = 11.82AAGEE17 pKa = 4.41GSFWWHH23 pKa = 7.34DD24 pKa = 3.84SATDD28 pKa = 3.5AYY30 pKa = 10.88LITDD34 pKa = 3.82TLEE37 pKa = 4.12PPAADD42 pKa = 3.72AVYY45 pKa = 10.84LMPWDD50 pKa = 4.57DD51 pKa = 3.07AWFVQWEE58 pKa = 4.3EE59 pKa = 4.12DD60 pKa = 2.97WTAAADD66 pKa = 3.69QLNSVIRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84LEE77 pKa = 4.04EE78 pKa = 4.11EE79 pKa = 3.61PHH81 pKa = 5.75

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V5R8|A0A649V5R8_9CAUD DNA methylase OS=Gordonia phage Wocket OX=2656543 GN=66 PE=4 SV=1
MM1 pKa = 7.57ARR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84PNPEE9 pKa = 3.22QRR11 pKa = 11.84GLGHH15 pKa = 5.41THH17 pKa = 6.1KK18 pKa = 10.78QQVKK22 pKa = 8.8HH23 pKa = 5.95LKK25 pKa = 7.95TRR27 pKa = 11.84HH28 pKa = 5.47VDD30 pKa = 3.6GTPCWWCGKK39 pKa = 9.42PMYY42 pKa = 10.3LDD44 pKa = 3.19RR45 pKa = 11.84TRR47 pKa = 11.84NPDD50 pKa = 3.69YY51 pKa = 11.07NPTSPDD57 pKa = 3.55PASGSLAGDD66 pKa = 3.31HH67 pKa = 5.93TLARR71 pKa = 11.84ARR73 pKa = 11.84GGTQADD79 pKa = 3.87RR80 pKa = 11.84LLHH83 pKa = 5.46GRR85 pKa = 11.84CNKK88 pKa = 9.38QRR90 pKa = 11.84GDD92 pKa = 3.25GRR94 pKa = 11.84HH95 pKa = 5.79DD96 pKa = 3.32HH97 pKa = 6.33TRR99 pKa = 11.84PALTGKK105 pKa = 8.98HH106 pKa = 4.95QPTPTTHH113 pKa = 7.2LAINCWPP120 pKa = 3.68

Molecular weight: 13.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

16295

39

1828

206.3

22.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.746 ± 0.485

0.841 ± 0.138

7.021 ± 0.374

5.799 ± 0.274

2.749 ± 0.14

8.23 ± 0.365

2.105 ± 0.16

4.59 ± 0.172

3.191 ± 0.252

7.806 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.179 ± 0.163

2.835 ± 0.144

5.983 ± 0.254

3.32 ± 0.134

7.542 ± 0.406

5.044 ± 0.182

6.965 ± 0.224

7.726 ± 0.286

2.068 ± 0.132

2.258 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski