Shewanella oneidensis (strain MR-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella; Shewanella oneidensis

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4068 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8EFB5|HIS4_SHEON 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase OS=Shewanella oneidensis (strain MR-1) OX=211586 GN=hisA PE=3 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84LTLTLEE8 pKa = 4.22TLLSQLGSFSWHH20 pKa = 6.1LDD22 pKa = 3.07VYY24 pKa = 10.78KK25 pKa = 10.87ALEE28 pKa = 4.1QPLNPTMAVLIIDD41 pKa = 4.64DD42 pKa = 4.06EE43 pKa = 4.78LEE45 pKa = 4.1EE46 pKa = 5.28EE47 pKa = 5.3RR48 pKa = 11.84DD49 pKa = 3.82QQDD52 pKa = 4.64DD53 pKa = 3.77PLDD56 pKa = 4.24PQTQGYY62 pKa = 9.64QYY64 pKa = 10.27FLSIADD70 pKa = 3.89LQSIKK75 pKa = 11.13ANLEE79 pKa = 3.76QQMPNATLHH88 pKa = 6.43DD89 pKa = 5.56LIHH92 pKa = 6.28AVSYY96 pKa = 11.1YY97 pKa = 10.61DD98 pKa = 3.68QTDD101 pKa = 2.95AYY103 pKa = 10.0MNLEE107 pKa = 4.11SS108 pKa = 4.47

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4PSK8|K4PSK8_SHEON Uncharacterized protein OS=Shewanella oneidensis (strain MR-1) OX=211586 GN=SO_4814 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATAGGRR29 pKa = 11.84KK30 pKa = 8.78VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4068

0

4068

1342432

16

5020

330.0

36.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.506 ± 0.038

1.075 ± 0.017

5.351 ± 0.03

5.769 ± 0.033

3.959 ± 0.025

6.863 ± 0.036

2.289 ± 0.022

6.068 ± 0.03

5.056 ± 0.036

10.911 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.58 ± 0.022

4.088 ± 0.032

4.081 ± 0.023

4.931 ± 0.036

4.599 ± 0.036

6.435 ± 0.037

5.408 ± 0.048

6.764 ± 0.038

1.253 ± 0.013

3.013 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski