Sanxia water strider virus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Lispiviridae; Arlivirus; Gerrid arlivirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KXD7|A0A0B5KXD7_9MONO ORF2 OS=Sanxia water strider virus 4 OX=1608063 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.83ANIDD5 pKa = 4.43DD6 pKa = 4.14NLEE9 pKa = 3.98TTFRR13 pKa = 11.84RR14 pKa = 11.84SIKK17 pKa = 9.75KK18 pKa = 9.53NKK20 pKa = 8.77RR21 pKa = 11.84DD22 pKa = 3.43RR23 pKa = 11.84VLTSDD28 pKa = 3.77CSVLINLIGSLHH40 pKa = 5.34TLIEE44 pKa = 4.22NTGGKK49 pKa = 9.56VNPALKK55 pKa = 10.53KK56 pKa = 7.26MTSNAPHH63 pKa = 6.83DD64 pKa = 4.71NIVEE68 pKa = 4.17EE69 pKa = 4.76EE70 pKa = 4.35GSGMKK75 pKa = 8.69EE76 pKa = 4.0TIEE79 pKa = 4.23GFQSQEE85 pKa = 3.79FEE87 pKa = 4.65LNDD90 pKa = 4.32LLVEE94 pKa = 4.76DD95 pKa = 4.68PSKK98 pKa = 10.66LTVEE102 pKa = 4.62MAQVMRR108 pKa = 11.84EE109 pKa = 4.28SISEE113 pKa = 3.91HH114 pKa = 6.61RR115 pKa = 11.84DD116 pKa = 3.02YY117 pKa = 11.41DD118 pKa = 3.88SEE120 pKa = 4.57VIQGEE125 pKa = 4.2AQDD128 pKa = 4.54LEE130 pKa = 4.84VPAVDD135 pKa = 5.21MRR137 pKa = 11.84DD138 pKa = 3.42EE139 pKa = 4.66DD140 pKa = 5.35LSLLDD145 pKa = 5.74DD146 pKa = 4.3VIEE149 pKa = 4.91PYY151 pKa = 10.74EE152 pKa = 4.73HH153 pKa = 6.25MWQMYY158 pKa = 6.48THH160 pKa = 6.93VLTLIEE166 pKa = 4.21RR167 pKa = 11.84EE168 pKa = 4.13EE169 pKa = 4.45IKK171 pKa = 10.93NAWDD175 pKa = 3.67TIYY178 pKa = 11.11NFYY181 pKa = 10.59PEE183 pKa = 4.79EE184 pKa = 4.5SPLTRR189 pKa = 11.84FNTLMLVKK197 pKa = 10.46LQDD200 pKa = 3.25HH201 pKa = 5.2VHH203 pKa = 5.7RR204 pKa = 11.84EE205 pKa = 3.83KK206 pKa = 11.04RR207 pKa = 11.84LVEE210 pKa = 3.98QVDD213 pKa = 3.71RR214 pKa = 11.84KK215 pKa = 10.52LDD217 pKa = 3.66SLSTKK222 pKa = 9.1VTVDD226 pKa = 2.74TDD228 pKa = 3.98NIIKK232 pKa = 9.68EE233 pKa = 4.03IKK235 pKa = 9.72KK236 pKa = 9.61IQIKK240 pKa = 6.34TTSTPIPAIAVSSPSTSKK258 pKa = 11.07NINLPKK264 pKa = 10.43AGTGTPTISGDD275 pKa = 3.81DD276 pKa = 3.74FLNKK280 pKa = 10.19LKK282 pKa = 10.42EE283 pKa = 3.86LRR285 pKa = 11.84KK286 pKa = 10.48NKK288 pKa = 7.78TAPVKK293 pKa = 10.04RR294 pKa = 11.84DD295 pKa = 3.17VVIIPKK301 pKa = 8.45EE302 pKa = 3.97TLKK305 pKa = 10.88FNPIILGPEE314 pKa = 4.15VQSKK318 pKa = 9.61PVVRR322 pKa = 11.84FTKK325 pKa = 10.42DD326 pKa = 3.1VSRR329 pKa = 11.84VSAAVIMEE337 pKa = 4.47EE338 pKa = 4.45KK339 pKa = 10.79SKK341 pKa = 9.92TDD343 pKa = 3.36KK344 pKa = 11.32YY345 pKa = 9.29WDD347 pKa = 3.61KK348 pKa = 11.08LISSGFLLDD357 pKa = 3.77RR358 pKa = 11.84EE359 pKa = 4.6KK360 pKa = 11.31VDD362 pKa = 4.26ALYY365 pKa = 11.29NSLKK369 pKa = 10.66LNKK372 pKa = 9.72EE373 pKa = 4.18LFNGLLDD380 pKa = 4.28PNNLDD385 pKa = 3.65RR386 pKa = 11.84SHH388 pKa = 6.38HH389 pKa = 5.86QGMIDD394 pKa = 4.01YY395 pKa = 9.4IWKK398 pKa = 9.78EE399 pKa = 3.7LGDD402 pKa = 3.64VGKK405 pKa = 10.63QSDD408 pKa = 4.68FIDD411 pKa = 4.75LMNHH415 pKa = 5.28MKK417 pKa = 8.97KK418 pKa = 6.75TTYY421 pKa = 9.3FVAIIRR427 pKa = 11.84YY428 pKa = 7.44WNLLEE433 pKa = 4.17

Molecular weight: 49.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KK30|A0A0B5KK30_9MONO RNA-directed RNA polymerase L OS=Sanxia water strider virus 4 OX=1608063 GN=L PE=3 SV=1
MM1 pKa = 7.33VSNHH5 pKa = 5.3SWKK8 pKa = 10.41CVIIILVNFTTMHH21 pKa = 6.98GDD23 pKa = 4.68LIFPDD28 pKa = 3.51IGYY31 pKa = 8.28MCEE34 pKa = 3.79PTRR37 pKa = 11.84FMMFSTGSEE46 pKa = 4.12TFHH49 pKa = 7.82IGLKK53 pKa = 10.14LPNIRR58 pKa = 11.84HH59 pKa = 6.26KK60 pKa = 11.1GADD63 pKa = 3.92CIKK66 pKa = 10.12EE67 pKa = 4.13SPEE70 pKa = 3.7VSNLLEE76 pKa = 4.15EE77 pKa = 5.92LYY79 pKa = 10.88DD80 pKa = 4.02LPQKK84 pKa = 10.38VIPNMNFLKK93 pKa = 10.47EE94 pKa = 3.73ISPRR98 pKa = 11.84SNFTRR103 pKa = 11.84DD104 pKa = 3.03KK105 pKa = 11.02RR106 pKa = 11.84FLTAIIGVVSLLSLGTSLYY125 pKa = 11.14GLGKK129 pKa = 10.11QYY131 pKa = 11.14SFEE134 pKa = 4.35SDD136 pKa = 2.63IDD138 pKa = 3.89DD139 pKa = 4.56KK140 pKa = 11.31IRR142 pKa = 11.84RR143 pKa = 11.84QDD145 pKa = 3.47LALHH149 pKa = 6.07EE150 pKa = 5.15LMIKK154 pKa = 10.21HH155 pKa = 5.68NQLLTVLDD163 pKa = 4.06EE164 pKa = 4.64QSNEE168 pKa = 3.81LKK170 pKa = 10.99NLGSSVDD177 pKa = 3.44MLHH180 pKa = 7.47RR181 pKa = 11.84IIEE184 pKa = 4.75NITCIQDD191 pKa = 3.36EE192 pKa = 4.97KK193 pKa = 10.59IQQLEE198 pKa = 4.59TITYY202 pKa = 8.32IQFFILKK209 pKa = 9.82LEE211 pKa = 4.27SFVYY215 pKa = 10.26AALKK219 pKa = 10.63GDD221 pKa = 4.03LHH223 pKa = 7.02PDD225 pKa = 3.45LVTLSMIRR233 pKa = 11.84TLIKK237 pKa = 10.14QRR239 pKa = 11.84SSLRR243 pKa = 11.84DD244 pKa = 3.34TLLDD248 pKa = 3.72KK249 pKa = 11.2DD250 pKa = 3.91PSVLYY255 pKa = 10.58NVGKK259 pKa = 10.36VIPLSYY265 pKa = 10.48NHH267 pKa = 6.34AHH269 pKa = 6.66RR270 pKa = 11.84VITLMIIMPIIRR282 pKa = 11.84LDD284 pKa = 4.04QISHH288 pKa = 7.18IYY290 pKa = 9.96DD291 pKa = 3.55CSNIGFKK298 pKa = 10.76KK299 pKa = 10.59NNTLFKK305 pKa = 9.98YY306 pKa = 7.72TLPEE310 pKa = 4.02NIITVKK316 pKa = 10.48VDD318 pKa = 3.43GKK320 pKa = 10.77QVMTSVNLQYY330 pKa = 10.96CHH332 pKa = 6.44TSGIIVMCNVNAIPTTNYY350 pKa = 10.13QCLDD354 pKa = 3.66DD355 pKa = 5.11LNNNVSSNCSPKK367 pKa = 10.73LLDD370 pKa = 3.75NLLPVAINTQQHH382 pKa = 4.15IMLRR386 pKa = 11.84GFSRR390 pKa = 11.84YY391 pKa = 8.93SVHH394 pKa = 5.62TQSTIPRR401 pKa = 11.84YY402 pKa = 10.01GSYY405 pKa = 10.47KK406 pKa = 10.26INLNPDD412 pKa = 3.16QNTVIAIDD420 pKa = 3.39KK421 pKa = 8.78GKK423 pKa = 10.44KK424 pKa = 8.91YY425 pKa = 10.94VVGGDD430 pKa = 2.7IFTNNVVKK438 pKa = 10.72ANYY441 pKa = 8.75DD442 pKa = 3.13IPTNLSIKK450 pKa = 9.87TMVKK454 pKa = 10.4NISLNNKK461 pKa = 9.08EE462 pKa = 4.59LDD464 pKa = 4.09NISKK468 pKa = 10.06EE469 pKa = 4.04IQKK472 pKa = 10.3LRR474 pKa = 11.84IEE476 pKa = 5.09NISDD480 pKa = 3.3WDD482 pKa = 3.67VSDD485 pKa = 3.88PSTYY489 pKa = 10.73HH490 pKa = 7.0FITIYY495 pKa = 10.35TLLLVSIAALLAISWCYY512 pKa = 10.68LRR514 pKa = 11.84GHH516 pKa = 7.98LIRR519 pKa = 11.84KK520 pKa = 8.01NKK522 pKa = 9.44SKK524 pKa = 10.63KK525 pKa = 8.71FKK527 pKa = 10.31KK528 pKa = 9.99IVMQHH533 pKa = 5.65SNNEE537 pKa = 3.96DD538 pKa = 3.03SDD540 pKa = 3.84

Molecular weight: 61.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3489

408

2108

872.3

100.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.783 ± 0.519

1.748 ± 0.327

5.818 ± 0.696

6.048 ± 0.726

4.385 ± 0.499

4.328 ± 0.199

2.522 ± 0.15

8.885 ± 0.512

7.223 ± 0.511

11.235 ± 0.327

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.752 ± 0.085

5.99 ± 0.464

3.239 ± 0.258

3.124 ± 0.214

4.471 ± 0.253

7.509 ± 0.374

6.22 ± 0.455

5.876 ± 0.34

1.146 ± 0.22

3.697 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski