Bacteroides sp. CAG:545

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2183 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5RVL8|R5RVL8_9BACE Uncharacterized protein OS=Bacteroides sp. CAG:545 OX=1262742 GN=BN702_00012 PE=4 SV=1
MM1 pKa = 7.69NYY3 pKa = 9.79QKK5 pKa = 10.75PQMDD9 pKa = 4.33VLVLGCDD16 pKa = 3.28EE17 pKa = 4.81RR18 pKa = 11.84FAASTISTDD27 pKa = 3.11QIYY30 pKa = 10.21FEE32 pKa = 5.17EE33 pKa = 4.82GTMDD37 pKa = 3.98NDD39 pKa = 4.69FNTEE43 pKa = 3.77NNN45 pKa = 3.47

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5RQ78|R5RQ78_9BACE Dihydrodipicolinate reductase OS=Bacteroides sp. CAG:545 OX=1262742 GN=BN702_01599 PE=3 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.39KK6 pKa = 9.62RR7 pKa = 11.84KK8 pKa = 6.72RR9 pKa = 11.84HH10 pKa = 5.17KK11 pKa = 10.05MATHH15 pKa = 6.02KK16 pKa = 10.34RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.28NRR25 pKa = 11.84HH26 pKa = 4.81KK27 pKa = 10.86NKK29 pKa = 10.25KK30 pKa = 8.92KK31 pKa = 10.55

Molecular weight: 3.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2183

0

2183

809031

30

2497

370.6

41.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.961 ± 0.052

1.343 ± 0.022

6.11 ± 0.038

6.294 ± 0.048

4.422 ± 0.033

7.398 ± 0.05

1.645 ± 0.02

6.255 ± 0.04

6.396 ± 0.043

8.399 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.024

4.626 ± 0.043

3.811 ± 0.029

2.735 ± 0.027

4.626 ± 0.037

7.143 ± 0.047

5.6 ± 0.04

7.007 ± 0.04

1.288 ± 0.02

4.217 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski