Hubei tombus-like virus 13

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KGP1|A0A1L3KGP1_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 13 OX=1923259 PE=4 SV=1
MM1 pKa = 7.47VIGEE5 pKa = 4.18QRR7 pKa = 11.84CLYY10 pKa = 9.98WGDD13 pKa = 3.64CTRR16 pKa = 11.84DD17 pKa = 2.73SGYY20 pKa = 10.84RR21 pKa = 11.84VTPSVALAKK30 pKa = 10.29FDD32 pKa = 3.89DD33 pKa = 4.06VKK35 pKa = 11.1RR36 pKa = 11.84PGRR39 pKa = 11.84EE40 pKa = 3.89VCVHH44 pKa = 6.21CGGVEE49 pKa = 4.41FVCCKK54 pKa = 10.23NPNIVSDD61 pKa = 3.63QVSRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84TSVACLNCEE76 pKa = 3.94AVHH79 pKa = 6.34QVGPNNFILYY89 pKa = 8.67DD90 pKa = 3.95AKK92 pKa = 10.66WQDD95 pKa = 3.89DD96 pKa = 4.03CRR98 pKa = 11.84CQCDD102 pKa = 3.38RR103 pKa = 11.84FVGRR107 pKa = 11.84SWGTQSTRR115 pKa = 11.84CHH117 pKa = 5.9SARR120 pKa = 11.84HH121 pKa = 4.89QKK123 pKa = 10.66ANPKK127 pKa = 9.9LGSKK131 pKa = 7.34TRR133 pKa = 11.84RR134 pKa = 11.84AGGNSKK140 pKa = 10.55KK141 pKa = 10.22IIRR144 pKa = 11.84EE145 pKa = 3.79SRR147 pKa = 11.84EE148 pKa = 3.57VNKK151 pKa = 10.01PFRR154 pKa = 11.84DD155 pKa = 3.9TPKK158 pKa = 10.04KK159 pKa = 9.19PGMMKK164 pKa = 8.91VTNGNGPALKK174 pKa = 10.52VITGPPAIKK183 pKa = 9.16EE184 pKa = 4.42TIDD187 pKa = 5.28DD188 pKa = 3.72ICKK191 pKa = 9.97LHH193 pKa = 6.48AGQWTTHH200 pKa = 4.71RR201 pKa = 11.84NKK203 pKa = 10.34RR204 pKa = 11.84RR205 pKa = 11.84VVLFGKK211 pKa = 10.62GSYY214 pKa = 9.19TYY216 pKa = 11.03SDD218 pKa = 3.29TVLRR222 pKa = 11.84ALEE225 pKa = 4.15KK226 pKa = 10.63PGWLLALEE234 pKa = 4.44KK235 pKa = 10.56VVKK238 pKa = 10.56ARR240 pKa = 11.84LQDD243 pKa = 3.61PLRR246 pKa = 11.84NLWIEE251 pKa = 4.18GQFDD255 pKa = 3.66TALVNHH261 pKa = 5.87YY262 pKa = 9.19PKK264 pKa = 10.88GVGIPPHH271 pKa = 6.57NDD273 pKa = 2.79AEE275 pKa = 4.27EE276 pKa = 4.84EE277 pKa = 4.14IDD279 pKa = 3.67QSAHH283 pKa = 4.33VVSYY287 pKa = 10.98SIGDD291 pKa = 3.59TVRR294 pKa = 11.84FSVKK298 pKa = 9.8KK299 pKa = 10.62GKK301 pKa = 8.57ATPVSTLLHH310 pKa = 6.6DD311 pKa = 4.07GEE313 pKa = 4.44IAVMEE318 pKa = 4.62SGFQADD324 pKa = 3.65YY325 pKa = 9.13THH327 pKa = 7.05WAEE330 pKa = 4.27GPLNEE335 pKa = 4.45RR336 pKa = 11.84VSVTWRR342 pKa = 11.84KK343 pKa = 9.26LLPSGEE349 pKa = 5.16DD350 pKa = 2.95ILEE353 pKa = 4.4NPSGGVPEE361 pKa = 4.65STPPTPPVGVRR372 pKa = 11.84CDD374 pKa = 3.73EE375 pKa = 4.03EE376 pKa = 5.04VEE378 pKa = 4.08ALFAKK383 pKa = 10.01VDD385 pKa = 3.44KK386 pKa = 9.51EE387 pKa = 4.27TEE389 pKa = 3.74EE390 pKa = 4.36FYY392 pKa = 11.07EE393 pKa = 4.01RR394 pKa = 11.84RR395 pKa = 11.84RR396 pKa = 11.84LSRR399 pKa = 11.84EE400 pKa = 3.53EE401 pKa = 4.69KK402 pKa = 9.58EE403 pKa = 4.23AQEE406 pKa = 4.18SFINAWCHH414 pKa = 4.57EE415 pKa = 4.06AQEE418 pKa = 4.39QEE420 pKa = 4.32EE421 pKa = 4.71FTSKK425 pKa = 11.01PEE427 pKa = 3.99VIQNIMKK434 pKa = 9.65HH435 pKa = 4.63WCLDD439 pKa = 3.61SLCYY443 pKa = 9.5RR444 pKa = 11.84ACEE447 pKa = 4.26KK448 pKa = 10.59RR449 pKa = 11.84HH450 pKa = 5.19SCPTRR455 pKa = 11.84FAKK458 pKa = 10.52YY459 pKa = 10.15AIDD462 pKa = 3.86FGVEE466 pKa = 3.86VALKK470 pKa = 8.95ATKK473 pKa = 10.26DD474 pKa = 3.55PQVSHH479 pKa = 6.61GALEE483 pKa = 4.75GDD485 pKa = 4.27CSQGNPSRR493 pKa = 11.84KK494 pKa = 9.2ADD496 pKa = 3.62GGSVEE501 pKa = 4.03DD502 pKa = 3.82RR503 pKa = 11.84EE504 pKa = 4.18RR505 pKa = 11.84EE506 pKa = 3.73EE507 pKa = 3.97RR508 pKa = 11.84EE509 pKa = 3.51RR510 pKa = 11.84RR511 pKa = 11.84RR512 pKa = 11.84VRR514 pKa = 11.84DD515 pKa = 3.35RR516 pKa = 11.84EE517 pKa = 3.89RR518 pKa = 11.84EE519 pKa = 3.82EE520 pKa = 4.03RR521 pKa = 11.84EE522 pKa = 3.39EE523 pKa = 4.07RR524 pKa = 11.84EE525 pKa = 3.62RR526 pKa = 11.84RR527 pKa = 11.84EE528 pKa = 3.59RR529 pKa = 11.84DD530 pKa = 3.07GVEE533 pKa = 3.92RR534 pKa = 11.84LRR536 pKa = 11.84GCPVRR541 pKa = 11.84VQEE544 pKa = 4.72PVGEE548 pKa = 4.28EE549 pKa = 3.63QTPDD553 pKa = 3.44YY554 pKa = 10.39TSVGVPEE561 pKa = 4.61EE562 pKa = 4.23DD563 pKa = 3.05EE564 pKa = 4.51GMGVAFGGEE573 pKa = 4.0IKK575 pKa = 10.77AGWFGNIGRR584 pKa = 11.84ACVRR588 pKa = 11.84VRR590 pKa = 11.84QSFNNKK596 pKa = 8.78LCNLYY601 pKa = 10.82GLLGAKK607 pKa = 9.91RR608 pKa = 11.84EE609 pKa = 4.14VHH611 pKa = 5.49VLPRR615 pKa = 11.84VAGEE619 pKa = 3.95HH620 pKa = 6.11GGAVQGTEE628 pKa = 3.74ALQLEE633 pKa = 4.78NLRR636 pKa = 11.84PGPVRR641 pKa = 11.84QLQSATDD648 pKa = 3.47VGCVQRR654 pKa = 11.84AVPEE658 pKa = 4.23LQHH661 pKa = 6.49RR662 pKa = 11.84APLGRR667 pKa = 11.84EE668 pKa = 3.6IVSEE672 pKa = 4.05RR673 pKa = 11.84KK674 pKa = 9.52VDD676 pKa = 3.78AEE678 pKa = 4.03GTLRR682 pKa = 11.84NDD684 pKa = 2.76RR685 pKa = 11.84RR686 pKa = 11.84VVSEE690 pKa = 3.96TKK692 pKa = 9.4PAKK695 pKa = 9.28EE696 pKa = 5.0AIRR699 pKa = 11.84QEE701 pKa = 4.22YY702 pKa = 9.21PPLAVNTRR710 pKa = 11.84PTQSRR715 pKa = 11.84QCSTVHH721 pKa = 7.31RR722 pKa = 11.84EE723 pKa = 3.93EE724 pKa = 5.43HH725 pKa = 6.83DD726 pKa = 3.44EE727 pKa = 3.99QAASRR732 pKa = 11.84GVLGSAQVEE741 pKa = 4.55TGKK744 pKa = 10.64GHH746 pKa = 7.13AKK748 pKa = 10.08GAEE751 pKa = 4.15KK752 pKa = 10.23EE753 pKa = 4.26KK754 pKa = 11.06KK755 pKa = 10.01PIAQEE760 pKa = 4.03VMEE763 pKa = 4.7SMVPRR768 pKa = 11.84AGKK771 pKa = 10.27KK772 pKa = 9.33ILPRR776 pKa = 11.84NYY778 pKa = 10.02KK779 pKa = 10.25EE780 pKa = 3.94EE781 pKa = 4.25LNAFLVQQCAFGGRR795 pKa = 11.84TVSDD799 pKa = 3.49TYY801 pKa = 11.7VLNKK805 pKa = 10.05KK806 pKa = 10.42AIDD809 pKa = 3.33WLEE812 pKa = 3.88QFDD815 pKa = 4.93LEE817 pKa = 4.55ASGITSAEE825 pKa = 3.86QFKK828 pKa = 10.89LVTLEE833 pKa = 4.05ASTNAILDD841 pKa = 3.68FEE843 pKa = 4.45QAKK846 pKa = 10.23KK847 pKa = 10.99LNDD850 pKa = 3.35RR851 pKa = 11.84MFEE854 pKa = 4.12VADD857 pKa = 3.68YY858 pKa = 10.53LQLINGFLRR867 pKa = 11.84KK868 pKa = 9.87GNAFGCVVPMWALAIVFCCLWFVSWYY894 pKa = 10.45GVYY897 pKa = 9.22GTIKK901 pKa = 10.51AVWIPLDD908 pKa = 3.45YY909 pKa = 11.05VRR911 pKa = 11.84YY912 pKa = 8.77NNNRR916 pKa = 11.84VLVAYY921 pKa = 7.47VTACACDD928 pKa = 3.62CLLAVIGVWALKK940 pKa = 10.78AFLRR944 pKa = 11.84CIGKK948 pKa = 9.92CGLRR952 pKa = 11.84RR953 pKa = 11.84FRR955 pKa = 11.84CRR957 pKa = 11.84FNNPLLEE964 pKa = 4.48RR965 pKa = 11.84YY966 pKa = 8.84GRR968 pKa = 11.84KK969 pKa = 8.02TYY971 pKa = 8.08TKK973 pKa = 9.07RR974 pKa = 11.84WTVTARR980 pKa = 11.84HH981 pKa = 5.39RR982 pKa = 11.84VVACSCGPLHH992 pKa = 6.01QRR994 pKa = 11.84WMDD997 pKa = 3.74GEE999 pKa = 4.21ASCPRR1004 pKa = 11.84RR1005 pKa = 11.84TMCGGCFACTEE1016 pKa = 4.13APVVGWRR1023 pKa = 11.84NWFKK1027 pKa = 10.79RR1028 pKa = 11.84PEE1030 pKa = 4.12YY1031 pKa = 10.8DD1032 pKa = 4.37DD1033 pKa = 4.69VRR1035 pKa = 11.84QEE1037 pKa = 5.06DD1038 pKa = 4.33EE1039 pKa = 4.38LLYY1042 pKa = 10.67DD1043 pKa = 3.8YY1044 pKa = 10.88GNCPGEE1050 pKa = 3.78GHH1052 pKa = 6.54EE1053 pKa = 5.49FLFEE1057 pKa = 5.42DD1058 pKa = 4.66GEE1060 pKa = 4.51RR1061 pKa = 11.84VAGQDD1066 pKa = 3.41RR1067 pKa = 11.84MEE1069 pKa = 4.1YY1070 pKa = 9.77CINWDD1075 pKa = 3.1EE1076 pKa = 4.24MYY1078 pKa = 11.07EE1079 pKa = 4.26DD1080 pKa = 4.96GEE1082 pKa = 4.27PMDD1085 pKa = 4.7IEE1087 pKa = 4.9CEE1089 pKa = 4.17APCTRR1094 pKa = 11.84KK1095 pKa = 10.1GRR1097 pKa = 11.84ICGEE1101 pKa = 3.61VRR1103 pKa = 11.84ILNWEE1108 pKa = 4.2FPAWCEE1114 pKa = 3.79RR1115 pKa = 11.84DD1116 pKa = 3.32IEE1118 pKa = 4.45EE1119 pKa = 4.11QCKK1122 pKa = 10.16EE1123 pKa = 3.99RR1124 pKa = 11.84VDD1126 pKa = 3.6NARR1129 pKa = 11.84IRR1131 pKa = 11.84VDD1133 pKa = 3.3KK1134 pKa = 10.97VYY1136 pKa = 10.8LGSRR1140 pKa = 11.84DD1141 pKa = 3.42FSKK1144 pKa = 10.52WLVIPTQWCLPNDD1157 pKa = 3.83GLIWLLVALFGVYY1170 pKa = 10.16KK1171 pKa = 10.19ILEE1174 pKa = 3.92YY1175 pKa = 11.03SNYY1178 pKa = 9.46VGFMHH1183 pKa = 6.52YY1184 pKa = 10.52VYY1186 pKa = 10.93ALYY1189 pKa = 8.8STCEE1193 pKa = 3.83YY1194 pKa = 10.69TGVQEE1199 pKa = 4.12WAHH1202 pKa = 5.54GVLGEE1207 pKa = 3.99TSPWEE1212 pKa = 4.14SLRR1215 pKa = 11.84EE1216 pKa = 3.9AGYY1219 pKa = 10.44EE1220 pKa = 3.67DD1221 pKa = 4.95WMKK1224 pKa = 10.96GWLGIEE1230 pKa = 4.19WVHH1233 pKa = 5.78VAPLIDD1239 pKa = 4.86RR1240 pKa = 11.84ISWSSHH1246 pKa = 3.47ACIAIVSGWSAALEE1260 pKa = 3.92IAALRR1265 pKa = 11.84RR1266 pKa = 11.84ALTFTKK1272 pKa = 10.45QIASPNN1278 pKa = 3.56

Molecular weight:
144.87 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KGP1|A0A1L3KGP1_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 13 OX=1923259 PE=4 SV=1
MM1 pKa = 7.63PVLGCAMPNKK11 pKa = 9.8KK12 pKa = 9.82LAEE15 pKa = 4.51GCAVTFVPNNLKK27 pKa = 10.55RR28 pKa = 11.84EE29 pKa = 3.99VTRR32 pKa = 11.84RR33 pKa = 11.84VGTVFARR40 pKa = 11.84PTVCEE45 pKa = 4.26KK46 pKa = 10.86EE47 pKa = 4.5FGLHH51 pKa = 3.89THH53 pKa = 5.57TVKK56 pKa = 11.1DD57 pKa = 3.95EE58 pKa = 4.01LQTICNRR65 pKa = 11.84HH66 pKa = 5.75LMEE69 pKa = 4.59TPQPVEE75 pKa = 5.3DD76 pKa = 3.64SLAWRR81 pKa = 11.84TFKK84 pKa = 10.91KK85 pKa = 10.2IMRR88 pKa = 11.84EE89 pKa = 3.76LSRR92 pKa = 11.84QIGKK96 pKa = 8.9VRR98 pKa = 11.84SAPLSAVLKK107 pKa = 10.01GKK109 pKa = 7.97TGRR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84KK115 pKa = 9.75RR116 pKa = 11.84FHH118 pKa = 6.62NGAAKK123 pKa = 10.25LRR125 pKa = 11.84LEE127 pKa = 4.16GVTRR131 pKa = 11.84KK132 pKa = 9.87DD133 pKa = 3.21ARR135 pKa = 11.84ITEE138 pKa = 4.12MQKK141 pKa = 10.89LEE143 pKa = 4.03MYY145 pKa = 8.84EE146 pKa = 4.05VNKK149 pKa = 10.42IPVKK153 pKa = 9.82EE154 pKa = 4.0DD155 pKa = 2.59RR156 pKa = 11.84GIQYY160 pKa = 10.51RR161 pKa = 11.84SVKK164 pKa = 10.61YY165 pKa = 10.28NVALAKK171 pKa = 9.86EE172 pKa = 3.85LHH174 pKa = 6.4NIEE177 pKa = 4.14KK178 pKa = 10.48RR179 pKa = 11.84LIGLHH184 pKa = 6.41EE185 pKa = 5.47DD186 pKa = 3.61GFHH189 pKa = 6.61PVMKK193 pKa = 10.32GATPQQRR200 pKa = 11.84AEE202 pKa = 4.32RR203 pKa = 11.84IALASLKK210 pKa = 9.81YY211 pKa = 10.22KK212 pKa = 10.73CPLYY216 pKa = 10.96LLLDD220 pKa = 3.74HH221 pKa = 6.93SRR223 pKa = 11.84FDD225 pKa = 3.46AHH227 pKa = 6.88VCYY230 pKa = 10.52LLLKK234 pKa = 10.6EE235 pKa = 5.08EE236 pKa = 3.7IAFYY240 pKa = 10.47KK241 pKa = 10.22RR242 pKa = 11.84CRR244 pKa = 11.84KK245 pKa = 9.88DD246 pKa = 3.16SRR248 pKa = 11.84YY249 pKa = 10.59LEE251 pKa = 4.19FLLNLQLKK259 pKa = 9.86NFGVSKK265 pKa = 10.86GGIRR269 pKa = 11.84YY270 pKa = 6.6KK271 pKa = 10.63TKK273 pKa = 9.83GKK275 pKa = 10.46RR276 pKa = 11.84MSGDD280 pKa = 2.87INTGVGNTVLNLAIIKK296 pKa = 9.94AWLRR300 pKa = 11.84VSGVKK305 pKa = 9.17GTIFLDD311 pKa = 3.98GDD313 pKa = 3.85DD314 pKa = 3.76SVVIIEE320 pKa = 4.55KK321 pKa = 10.63SDD323 pKa = 3.61FEE325 pKa = 4.12KK326 pKa = 10.92LLPVEE331 pKa = 4.64EE332 pKa = 5.12FMLQLGMVTEE342 pKa = 4.6MDD344 pKa = 3.5VTDD347 pKa = 4.83CFWKK351 pKa = 10.97AEE353 pKa = 4.25FCQSRR358 pKa = 11.84PVLVDD363 pKa = 2.77NTVRR367 pKa = 11.84YY368 pKa = 9.23VRR370 pKa = 11.84NPHH373 pKa = 6.33KK374 pKa = 10.9VLATVGQTAEE384 pKa = 4.14NVGKK388 pKa = 10.04EE389 pKa = 4.12VIEE392 pKa = 4.07EE393 pKa = 4.1VVRR396 pKa = 11.84ASCMCEE402 pKa = 3.14LALNGNCPVISPYY415 pKa = 10.05CRR417 pKa = 11.84RR418 pKa = 11.84LMGEE422 pKa = 4.21AKK424 pKa = 9.91TEE426 pKa = 3.84NTRR429 pKa = 11.84FNPSQLWKK437 pKa = 10.51AEE439 pKa = 3.82QYY441 pKa = 7.53GTKK444 pKa = 10.29FEE446 pKa = 4.28VPEE449 pKa = 4.18EE450 pKa = 4.03NEE452 pKa = 3.91PTVEE456 pKa = 4.04SRR458 pKa = 11.84VSFWRR463 pKa = 11.84AWDD466 pKa = 3.59IDD468 pKa = 3.53PSMQHH473 pKa = 6.64AMEE476 pKa = 4.32NQHH479 pKa = 7.06IIVGDD484 pKa = 3.73AQGSAKK490 pKa = 9.66KK491 pKa = 10.2RR492 pKa = 11.84KK493 pKa = 9.28SKK495 pKa = 10.13PIKK498 pKa = 9.68PDD500 pKa = 3.32EE501 pKa = 4.3EE502 pKa = 4.71LLEE505 pKa = 4.22EE506 pKa = 3.86WSGYY510 pKa = 8.86NEE512 pKa = 4.26PEE514 pKa = 4.73CGCGDD519 pKa = 3.89CPTYY523 pKa = 10.75GSEE526 pKa = 4.2EE527 pKa = 4.38AYY529 pKa = 10.52SAARR533 pKa = 11.84LWLKK537 pKa = 10.73

Molecular weight:
61.21 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1815

537

1278

907.5

103.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.107 ± 0.407

3.691 ± 0.374

4.573 ± 0.348

9.091 ± 0.08

3.196 ± 0.082

7.493 ± 0.512

2.204 ± 0.016

4.022 ± 0.137

6.336 ± 1.17

7.603 ± 0.799

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.541

3.802 ± 0.253

4.683 ± 0.112

3.416 ± 0.229

8.154 ± 0.468

4.738 ± 0.043

4.573 ± 0.239

8.32 ± 0.164

2.314 ± 0.433

2.92 ± 0.067

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski